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Genetics. Jun 1975; 80(2): 251–262.
PMCID: PMC1213325

Macronuclear Genetics of Tetrahymena. II. Macronuclear Location of Somatic Mutations to Cycloheximide Resistance

Abstract

Somatic cycloheximide-resistant mutants of syngen 1 of Tetrahymena pyriformis were isolated and genetically characterized. Two properties of the mutants were independently examined: (a) The transmission of the mutant phenotype during conjugation and (b) the kinetics of phenotypic assortment during vegetative propagation. The results of both studies strongly support the idea that these somatic mutations have a macronuclear location. The kinetics of assortment are consistent with the idea that the syngen 1 macronucleus contains about 45 assorting genetic units. The sib-selection method employed here, used in conjunction with the analysis of assortment kinetics and a previously described test for randomness of distribution, provides a probe of macronuclear genetics applicable to many ciliates, including those in which conjugation is not known to occur or is not under experimental control.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bruns PJ, Brussard TB. Positive selection for mating with functional heterokaryons in Tetrahymena pyriformis. Genetics. 1974 Nov;78(3):831–841. [PMC free article] [PubMed]
  • Cavalli-Sforza LL, Lederberg J. Isolation of Pre-Adaptive Mutants in Bacteria by Sib Selection. Genetics. 1956 May;41(3):367–381. [PMC free article] [PubMed]
  • Doerder FP. Regulatory Serotype Mutations in TETRAHYMENA PYRIFORMIS, Syngen 1. Genetics. 1973 May;74(1):81–106. [PMC free article] [PubMed]
  • McCoy JW. A Temperature-Sensitive Mutant in TETRAHYMENA PYRIFORMIS, Syngen 1. Genetics. 1973 May;74(1):107–114. [PMC free article] [PubMed]
  • Orias E, Flacks M. Use of genomic exclusion to isolate heat-sensitive mutants in Tetrahymena. Genetics. 1973 Apr;73(4):543–549. [PMC free article] [PubMed]

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