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Genetics. May 1975; 80(1): 41–59.
PMCID: PMC1213319

Mating Type and Sporulation in Yeast I. Mutations Which Alter Mating-Type Control over Sporulation

Abstract

In Saccharomyces cerevisiae, meiosis and spore formation as well as mating are controlled by mating-type genes. Diploids heterozygous for mating type (aα) can sporulate but cannot mate; homozygous aa and αα diploids can mate, but cannot sporulate. From an αα diploid parental strain, we have isolated mutants which have gained the ability to sporulate. Those mutants which continue to mate as αα cells have been designated CSP (control of sporulation). Upon sporulation, CSP mutants yield asci containing 4α spores. The mutant gene which allows αα cells to sporulate is unlinked to the mating-type locus and also acts to permit sporulation in aa diploid cells. Segregation data from crosses between mutant αα and wild-type aa diploids and vice versa indicate (for all but one mutant) that the mutation which allows constitutive sporulation (CSP) is dominant over the wild-type allele. Some of the CSP mutants are temperature-sensitive, sporulating at 32°, but not at 23°. In addition to CSP mutants, our mutagenesis and screening procedure led to the isolation of mutants which sporulate by virtue of a change in the mating-type locus itself, resulting in loss of ability to mate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • KISSANE JM, ROBINS E. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958 Jul;233(1):184–188. [PubMed]
  • Lindegren CC, Lindegren G. A New Method for Hybridizing Yeast. Proc Natl Acad Sci U S A. 1943 Oct 15;29(10):306–308. [PMC free article] [PubMed]
  • MILLER JJ, HOFFMANN-OSTENHOF C. SPORE FORMATION AND GERMINATION IN SACCHAROMYCES. Z Allg Mikrobiol. 1964;64:273–294. [PubMed]
  • Mortimer RK, Hawthorne DC. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. [PMC free article] [PubMed]
  • Roman H, Sands SM. Heterogeneity of Clones of Saccharomyces Derived from Haploid Ascospores. Proc Natl Acad Sci U S A. 1953 Mar;39(3):171–179. [PMC free article] [PubMed]
  • Roman H, Phillips MM, Sands SM. Studies of Polyploid Saccharomyces. I. Tetraploid Segregation. Genetics. 1955 Jul;40(4):546–561. [PMC free article] [PubMed]
  • Roth R. Chromosome replication during meiosis: identification of gene functions required for premeiotic DNA synthesis. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3087–3091. [PMC free article] [PubMed]
  • Roth R, Halvorson HO. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. [PMC free article] [PubMed]
  • Roth R, Lusnak K. DNA synthesis during yeast sporulation: genetic control of an early developmental event. Science. 1970 Apr 24;168(3930):493–494. [PubMed]
  • Roth R, Fogel S. A system selective for yeast mutants deficient in meiotic recombination. Mol Gen Genet. 1971;112(4):295–305. [PubMed]
  • SHERMAN F, ROMAN H. Evidence for two types of allelic recombination in yeast. Genetics. 1963 Feb;48:255–261. [PMC free article] [PubMed]
  • Simchen G, Salts Y, Piñon R. Sensitivity of meiotic yeast cells to ultraviolet light. Genetics. 1973 Apr;73(4):531–541. [PMC free article] [PubMed]

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