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Genetics. 1972 Jun; 71(2): 207–216.
PMCID: PMC1212778

On Some Genetic Aspects of Phage λ Resistance in E. COLI K12


Most mutations rendering E. coli K12 resistant to phage λ, map in two genetic regions malA and malB.—The malB region contains a gene lamB specifically involved in the λ receptor synthesis. Twenty-one independent lamB mutations studied by complementation belonged to a single cistron. This makes it very likely that lamB is monocistronic. Among the lamB mutants some are still sensitive to a host range mutant of phage λ. Mutations mapping in a proximal gene essential for maltose metabolism inactivate gene lamB by polarity confirming that both genes are part of the same operon. Because cases of intracistronic complementation have been found, the active lamB product may be an oligomeric protein.—Previously all λ resistant mutations in the malA region have been shown to map in the malT cistron. malT is believed to be a positive regulatory gene necessary for the induction of the "maltose operons" in the malA region and in the malB region of the E. coli K12 genetic map. No trans dominant malT mutation have been found. Therefore if they exist, they occur at a frequency of less than 10-8, or strongly reduce the growth rate of the mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • APPLEYARD RK, MCGREGOR JF, BAIRD KM. Mutation to extended host range and the occurrence of phenotypic mixing in the temperate coliphage lambda. Virology. 1956 Aug;2(4):565–574. [PubMed]
  • De Crombrugghe B, Chen B, Gottesman M, Pastan I, Varmus HE, Emmer M, Perlman RL. Regulation of lac mRNA synthesis in a soluble cell-free system. Nat New Biol. 1971 Mar 10;230(10):37–40. [PubMed]
  • Hatfield D, Hofnung M, Schwartz M. Genetic analysis of the maltose A region in Escherichia coli. J Bacteriol. 1969 May;98(2):559–567. [PMC free article] [PubMed]
  • Hofnung M, Schwartz M, Hatfield D. Complementation studies in the maltose-A region of the Escherichia coli K12 genetic map. J Mol Biol. 1971 Nov 14;61(3):681–694. [PubMed]
  • Hofnung M, Schwartz M. Mutations allowing growth on maltose of Escherichia coli K 12 strains with a deleted malT gene. Mol Gen Genet. 1971;112(2):117–132. [PubMed]
  • Howes WV. Effect of glucose on the capacity of Escherichia coli to be infected by a virulent lamba bacteriophage. J Bacteriol. 1965 Nov;90(5):1188–1193. [PMC free article] [PubMed]
  • JACOB F, WOLLMAN EL. Etude génétique d'un bactériophage tempéré d'Escherichia coli. l. Le système genétique du bactériophage. Ann Inst Pasteur (Paris) 1954 Dec;87(6):653–673. [PubMed]
  • Jordan E, Saedler H, Starlinger P. O0 and strong-polar mutations in the gal operon are insertions. Mol Gen Genet. 1968;102(4):353–363. [PubMed]
  • Malamy MH. Frameshift mutations in the lactose operon of E. coli. Cold Spring Harb Symp Quant Biol. 1966;31:189–201. [PubMed]
  • WEISSBACH A, JACOB F. Effect of glucose on the formation of bacteriophage lambda. Nature. 1962 Jan 13;193:197–198. [PubMed]

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