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Genetics. May 1997; 146(1): 149–164.
PMCID: PMC1207933

Natural Variation and Copulatory Plug Formation in Caenorhabditis Elegans

Abstract

Most of the available natural isolates of the nematode Caenorhabditis elegans have been examined and compared with the standard laboratory wild type (Bristol N2). Molecular markers, in particular transposon restriction fragment length polymorphisms, were used to assign these isolates to 22 different races, for which brood size and spontaneous male frequency were determined. Several distinctive traits were observed in some of these races. One example is mab-23, in a race from Vancouver, which leads to severe distortion of male genitalia and prevents male mating. Another is gro-1, segregating in a Californian race, which is associated with slow growth, heat resistance and longevity. Many races differ from N2 in carrying a dominant allele at the plg-1 locus, causing copulatory plug formation by males. Properties and possible advantages of the plugging trait have been investigated. The dominant plg-1 allele does not lead to increased male mating efficiency, but males from a Stanford race (CB4855), in which the plugging trait was first observed, are much more virile than N2 males. Crosses between N2 and CB4855 indicate that the higher virility is due to multiple factors. Size differences between N2 and CB4855 are associated with factors mapping to LGV and LGX.

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Selected References

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  • Babity JM, Starr TV, Rose AM. Tc1 transposition and mutator activity in a Bristol strain of Caenorhabditis elegans. Mol Gen Genet. 1990 Jun;222(1):65–70. [PubMed]
  • Barnes TM, Hodgkin J. The tra-3 sex determination gene of Caenorhabditis elegans encodes a member of the calpain regulatory protease family. EMBO J. 1996 Sep 2;15(17):4477–4484. [PMC free article] [PubMed]
  • Barton MK, Kimble J. fog-1, a regulatory gene required for specification of spermatogenesis in the germ line of Caenorhabditis elegans. Genetics. 1990 May;125(1):29–39. [PMC free article] [PubMed]
  • Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May;77(1):71–94. [PMC free article] [PubMed]
  • Church DL, Guan KL, Lambie EJ. Three genes of the MAP kinase cascade, mek-2, mpk-1/sur-1 and let-60 ras, are required for meiotic cell cycle progression in Caenorhabditis elegans. Development. 1995 Aug;121(8):2525–2535. [PubMed]
  • Dion M, Brun JL. Cartographie génique du nématode libre Caenorhabditis elegans, Maupas 1900, variété Bergerac. I. Etude de deux mutants nains. Mol Gen Genet. 1971;112(2):133–151. [PubMed]
  • Eide D, Anderson P. Transposition of Tc1 in the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1985 Mar;82(6):1756–1760. [PMC free article] [PubMed]
  • Emmons SW, Yesner L, Ruan KS, Katzenberg D. Evidence for a transposon in Caenorhabditis elegans. Cell. 1983 Jan;32(1):55–65. [PubMed]
  • Fatt HV, Dougherty EC. Genetic Control of Differential Heat Tolerance in Two Strains of the Nematode Caenorhabditis elegans. Science. 1963 Jul 19;141(3577):266–267. [PubMed]
  • Fitch DH, Bugaj-Gaweda B, Emmons SW. 18S ribosomal RNA gene phylogeny for some Rhabditidae related to Caenorhabditis. Mol Biol Evol. 1995 Mar;12(2):346–358. [PubMed]
  • Hartman PS, Herman RK. Radiation-sensitive mutants of Caenorhabditis elegans. Genetics. 1982 Oct;102(2):159–178. [PMC free article] [PubMed]
  • Hodgkin J. Novel nematode amber suppressors. Genetics. 1985 Oct;111(2):287–310. [PMC free article] [PubMed]
  • Hodgkin J. Molecular cloning and duplication of the nematode sex-determining gene tra-1. Genetics. 1993 Mar;133(3):543–560. [PMC free article] [PubMed]
  • Hodgkin J, Barnes TM. More is not better: brood size and population growth in a self-fertilizing nematode. Proc Biol Sci. 1991 Oct 22;246(1315):19–24. [PubMed]
  • Hodgkin J, Horvitz HR, Brenner S. Nondisjunction Mutants of the Nematode CAENORHABDITIS ELEGANS. Genetics. 1979 Jan;91(1):67–94. [PMC free article] [PubMed]
  • Horvitz HR, Brenner S, Hodgkin J, Herman RK. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet. 1979 Sep;175(2):129–133. [PubMed]
  • Kessin RH, Gundersen GG, Zaydfudim V, Grimson M. How cellular slime molds evade nematodes. Proc Natl Acad Sci U S A. 1996 May 14;93(10):4857–4861. [PMC free article] [PubMed]
  • Kondo K, Makovec B, Waterston RH, Hodgkin J. Genetic and molecular analysis of eight tRNA(Trp) amber suppressors in Caenorhabditis elegans. J Mol Biol. 1990 Sep 5;215(1):7–19. [PubMed]
  • Lakowski B, Hekimi S. Determination of life-span in Caenorhabditis elegans by four clock genes. Science. 1996 May 17;272(5264):1010–1013. [PubMed]
  • NIGON V, DOUGHERTY EC. Reproductive patterns and attempts at reciprocal crossing of Rhabditis elegans Maupas, 1900, and Rhabditis briggsae Dougherty and Nigon, 1949 (Nematoda: Rhabditidae). J Exp Zool. 1949 Dec;112(3):485–503. [PubMed]
  • Thomas JH, Birnby DA, Vowels JJ. Evidence for parallel processing of sensory information controlling dauer formation in Caenorhabditis elegans. Genetics. 1993 Aug;134(4):1105–1117. [PMC free article] [PubMed]
  • Thomas WK, Wilson AC. Mode and tempo of molecular evolution in the nematode caenorhabditis: cytochrome oxidase II and calmodulin sequences. Genetics. 1991 Jun;128(2):269–279. [PMC free article] [PubMed]
  • Ward S, Miwa J. Characterization of temperature-sensitive, fertilization-defective mutants of the nematode caenorhabditis elegans. Genetics. 1978 Feb;88(2):285–303. [PMC free article] [PubMed]
  • Waterston R, Sulston J. The genome of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10836–10840. [PMC free article] [PubMed]
  • Wong A, Boutis P, Hekimi S. Mutations in the clk-1 gene of Caenorhabditis elegans affect developmental and behavioral timing. Genetics. 1995 Mar;139(3):1247–1259. [PMC free article] [PubMed]
  • Wood WB, Hecht R, Carr S, Vanderslice R, Wolf N, Hirsh D. Parental effects and phenotypic characterization of mutations that affect early development in Caenorhabditis elegans. Dev Biol. 1980 Feb;74(2):446–469. [PubMed]

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