• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Dec 1996; 144(4): 1601–1610.
PMCID: PMC1207712

P-Element-Induced Recombination in Drosophila Melanogaster: Hybrid Element Insertion


It has previously been shown that the combination of two deleted P elements in trans, one containing the left functional end and the second element the right functional end, can lead to high levels of male recombination. This finding strongly suggests that P-element ends from different chromosomes can become associated, followed by ``pseudo-excision.'' We show that two different processes are involved in resolving the pseudo-excision event: (1) the excised P-element ends continue to function as a single unit (Hybrid Element) and insert at a nearby site in the chromosome or into the element itself [Hybrid Element Insertion (HEI)] and (2) free ends that do not contain P elements repair and rejoin [(Hybrid Excision and Repair (HER)]. Both types of resolution can lead to recombination, and this paper concentrates on the HEI class. One type of HEI event predicts the exact reverse complementary duplication of an 8-bp target site, and we have confirmed the existence of such a structure in six independently derived recombinant chromosomes. There is also a high tendency for insertion events to occur within a few bases of the original 8-bp target site, including six apparent cases of insertion into the exact site.

Full Text

The Full Text of this article is available as a PDF (3.3M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bingham PM, Kidwell MG, Rubin GM. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. [PubMed]
  • Engels WR, Johnson-Schlitz DM, Eggleston WB, Sved J. High-frequency P element loss in Drosophila is homolog dependent. Cell. 1990 Aug 10;62(3):515–525. [PubMed]
  • English J, Harrison K, Jones JD. A genetic analysis of DNA sequence requirements for Dissociation state I activity in tobacco. Plant Cell. 1993 May;5(5):501–514. [PMC free article] [PubMed]
  • Golic KG. Local transposition of P elements in Drosophila melanogaster and recombination between duplicated elements using a site-specific recombinase. Genetics. 1994 Jun;137(2):551–563. [PMC free article] [PubMed]
  • Hiraizumi Y. Spontaneous recombination in Drosophila melanogaster males. Proc Natl Acad Sci U S A. 1971 Feb;68(2):268–270. [PMC free article] [PubMed]
  • Johnson-Schlitz DM, Engels WR. P-element-induced interallelic gene conversion of insertions and deletions in Drosophila melanogaster. Mol Cell Biol. 1993 Nov;13(11):7006–7018. [PMC free article] [PubMed]
  • McCarron MY, Duttaroy A, Doughty GA, Chovnick A. P-element transposase induces male recombination in Drosophila melanogaster. Genet Res. 1989 Oct;54(2):137–141. [PubMed]
  • Nassif N, Penney J, Pal S, Engels WR, Gloor GB. Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair. Mol Cell Biol. 1994 Mar;14(3):1613–1625. [PMC free article] [PubMed]
  • O'Hare K, Rubin GM. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. [PubMed]
  • Pirrotta V. Vectors for P-mediated transformation in Drosophila. Biotechnology. 1988;10:437–456. [PubMed]
  • Preston CR, Sved JA, Engels WR. Flanking duplications and deletions associated with P-induced male recombination in Drosophila. Genetics. 1996 Dec;144(4):1623–1638. [PMC free article] [PubMed]
  • Roberts DE, Ascherman D, Kleckner N. IS10 promotes adjacent deletions at low frequency. Genetics. 1991 May;128(1):37–43. [PMC free article] [PubMed]
  • Sved JA, Eggleston WB, Engels WR. Germ-line and somatic recombination induced by in vitro modified P elements in Drosophila melanogaster. Genetics. 1990 Feb;124(2):331–337. [PMC free article] [PubMed]
  • Sved JA, Blackman LM, Gilchrist AS, Engels WR. High levels of recombination induced by homologous P elements in Drosophila melanogaster. Mol Gen Genet. 1991 Mar;225(3):443–447. [PubMed]
  • Tower J, Karpen GH, Craig N, Spradling AC. Preferential transposition of Drosophila P elements to nearby chromosomal sites. Genetics. 1993 Feb;133(2):347–359. [PMC free article] [PubMed]
  • Weil CF, Wessler SR. Molecular evidence that chromosome breakage by Ds elements is caused by aberrant transposition. Plant Cell. 1993 May;5(5):515–522. [PMC free article] [PubMed]
  • Zhang P, Spradling AC. Efficient and dispersed local P element transposition from Drosophila females. Genetics. 1993 Feb;133(2):361–373. [PMC free article] [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...