• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Aug 1996; 143(4): 1615–1627.
PMCID: PMC1207425

Response of Two Heat Shock Genes to Selection for Knockdown Heat Resistance in Drosophila Melanogaster

Abstract

To identify genes involved in stress resistance and heat hardening, replicate lines of Drosophila melanogaster were selected for increased resistance to knockdown by a 39° heat stress. Two selective regimes were used, one with and one without prior hardening. Mean knockdown times were increased from ~5 min to >20 min after 18 generations. Initial realized heritabilities were as high as 10% for lines selected without hardening, and crosses between lines indicated simple additive gene effects for the selected phenotypes. To survey allelic variation and correlated selection responses in two candidate stress genes, hsr-omega and hsp68, we applied denaturing gradient gel electrophoresis to amplified DNA sequences from small regions of these genes. After eight generations of selection, allele frequencies at both loci showed correlated responses for selection following hardening, but not without hardening. The hardening process itself was associated with a hsp68 frequency change in the opposite direction to that associated with selection that followed hardening. These stress loci are closely linked on chromosome III, and the hardening selection established a disequilibrium, suggesting an epistatic effect on resistance. The data indicate that molecular variation in both hsr-omega and hsp68 contribute to natural heritable variation for hardened heat resistance.

Full Text

The Full Text of this article is available as a PDF (2.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bendena WG, Ayme-Southgate A, Garbe JC, Pardue ML. Expression of heat-shock locus hsr-omega in nonstressed cells during development in Drosophila melanogaster. Dev Biol. 1991 Mar;144(1):65–77. [PubMed]
  • Bendena WG, Garbe JC, Traverse KL, Lakhotia SC, Pardue ML. Multiple inducers of the Drosophila heat shock locus 93D (hsr omega): inducer-specific patterns of the three transcripts. J Cell Biol. 1989 Jun;108(6):2017–2028. [PMC free article] [PubMed]
  • Bosch TC, Krylow SM, Bode HR, Steele RE. Thermotolerance and synthesis of heat shock proteins: these responses are present in Hydra attenuata but absent in Hydra oligactis. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7927–7931. [PMC free article] [PubMed]
  • Clos J, Westwood JT, Becker PB, Wilson S, Lambert K, Wu C. Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation. Cell. 1990 Nov 30;63(5):1085–1097. [PubMed]
  • Cohan FM, Hoffmann AA. Genetic divergence under uniform selection. II. Different responses to selection for knockdown resistance to ethanol among Drosophila melanogaster populations and their replicate lines. Genetics. 1986 Sep;114(1):145–164. [PMC free article] [PubMed]
  • Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 1991 Jul 25;19(14):4008–4008. [PMC free article] [PubMed]
  • Feder JH, Rossi JM, Solomon J, Solomon N, Lindquist S. The consequences of expressing hsp70 in Drosophila cells at normal temperatures. Genes Dev. 1992 Aug;6(8):1402–1413. [PubMed]
  • Fini ME, Bendena WG, Pardue ML. Unusual behavior of the cytoplasmic transcript of hsr omega: an abundant, stress-inducible RNA that is translated but yields no detectable protein product. J Cell Biol. 1989 Jun;108(6):2045–2057. [PMC free article] [PubMed]
  • Garbe JC, Bendena WG, Alfano M, Pardue ML. A Drosophila heat shock locus with a rapidly diverging sequence but a conserved structure. J Biol Chem. 1986 Dec 25;261(36):16889–16894. [PubMed]
  • Hoffmann AA, Parsons PA. Selection for increased desiccation resistance in Drosophila melanogaster: additive genetic control and correlated responses for other stresses. Genetics. 1989 Aug;122(4):837–845. [PMC free article] [PubMed]
  • Hogan NC, Slot F, Traverse KL, Garbe JC, Bendena WG, Pardue ML. Stability of tandem repeats in the Drosophila melanogaster Hsr-omega nuclear RNA. Genetics. 1995 Apr;139(4):1611–1621. [PMC free article] [PubMed]
  • Holmgren R, Livak K, Morimoto R, Freund R, Meselson M. Studies of cloned sequences from four Drosophila heat shock loci. Cell. 1979 Dec;18(4):1359–1370. [PubMed]
  • Holmgren R, Corces V, Morimoto R, Blackman R, Meselson M. Sequence homologies in the 5' regions of four Drosophila heat-shock genes. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3775–3778. [PMC free article] [PubMed]
  • Jenkins NL, Hoffmann AA. Genetic and maternal variation for heat resistance in Drosophila from the field. Genetics. 1994 Jul;137(3):783–789. [PMC free article] [PubMed]
  • Knibb WR, Oakeshott JG, Gibson JB. Chromosome Inversion Polymorphisms in DROSOPHILA MELANOGASTER. I. Latitudinal Clines and Associations between Inversions in Australasian Populations. Genetics. 1981 Aug;98(4):833–847. [PMC free article] [PubMed]
  • Lakhotia SC. The 93D heat shock locus of Drosophila melanogaster: modulation by genetic and developmental factors. Genome. 1989;31(2):677–683. [PubMed]
  • Myers RM, Maniatis T, Lerman LS. Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol. 1987;155:501–527. [PubMed]
  • Nagao RT, Kimpel JA, Key JL. Molecular and cellular biology of the heat-shock response. Adv Genet. 1990;28:235–274. [PubMed]
  • Palter KB, Watanabe M, Stinson L, Mahowald AP, Craig EA. Expression and localization of Drosophila melanogaster hsp70 cognate proteins. Mol Cell Biol. 1986 Apr;6(4):1187–1203. [PMC free article] [PubMed]
  • Parsons PA. Genetics of resistance to environmental stresses in Drosophila populations. Annu Rev Genet. 1973;7:239–265. [PubMed]
  • Ulmasov KA, Shammakov S, Karaev K, Evgen'ev MB. Heat shock proteins and thermoresistance in lizards. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1666–1670. [PMC free article] [PubMed]
  • Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 1991 Apr;10(4):506–513. [PubMed]
  • White CN, Hightower LE, Schultz RJ. Variation in heat-shock proteins among species of desert fishes (Poeciliidae, Poeciliopsis). Mol Biol Evol. 1994 Jan;11(1):106–119. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...