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Genetics. Apr 1996; 142(4): 1349–1355.
PMCID: PMC1207130

Dosage Effects on Gene Expression in a Maize Ploidy Series


Previous studies on gene expression in aneuploids revealed numerous trans-acting dosage effects. Segmental aneuploidy of each varied chromosomal region exhibited predominantly inverse effects on several target genes. Here, dosage regulation was examined in a maize (Zea mays L.) ploidy series where the complete genomic complement is varied. Total RNA from leaf tissue of monoploid, diploid, triploid, and tetraploid plants (1X, 2X, 3X, and 4X, respectively) was analyzed for the expression of 18 genes. For most tested genes, the transcript level per cell is directly proportional to structural gene dosage; that is, on a per genome basis, there is approximately equal expression among the four ploidies. Exceptional cases show a negative correlation of expression with ploidy or a positive correlation greater than expected from the structural gene dosage. These studies suggest that, in general, as structural gene dosage increases in multiples of the monoploid complement, the absolute level of gene expression per cell increases. In contrast, addition or subtraction of only a single chromosome arm tends to alter gene expression patterns extensively. The combined results of the euploid and aneuploid studies suggest that aneuploid effects result from an altered stoichiometry of the factors contributing to the mechanisms of gene expression.

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Selected References

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  • Birchler JA. A study of enzyme activities in a dosage series of the long arm of chromosome one in maize. Genetics. 1979 Aug;92(4):1211–1229. [PMC free article] [PubMed]
  • Birchler JA. The genetic basis of dosage compensation of alcohol dehydrogenase-1 in maize. Genetics. 1981 Mar;97(3-4):625–637. [PMC free article] [PubMed]
  • Birchler JA, Hiebert JC. Interaction of the Enhancer of white-apricot with transposable element alleles at the white locus in Drosophila melanogaster. Genetics. 1989 May;122(1):129–138. [PMC free article] [PubMed]
  • Birchler JA, Newton KJ. Modulation of protein levels in chromosomal dosage series of maize: the biochemical basis of aneuploid syndromes. Genetics. 1981 Oct;99(2):247–266. [PMC free article] [PubMed]
  • Birchler JA, Hiebert JC, Paigen K. Analysis of autosomal dosage compensation involving the alcohol dehydrogenase locus in Drosophila melanogaster. Genetics. 1990 Mar;124(3):679–686. [PMC free article] [PubMed]
  • Ciferri O, Sora S, Tiboni O. Effect of gene dosage on tryptophan synthetase activity in Saccharomyces cerevisiae. Genetics. 1969 Mar;61(3):567–576. [PMC free article] [PubMed]
  • Devlin RH, Holm DG, Grigliatti TA. The influence of whole-arm trisomy on gene expression in Drosophila. Genetics. 1988 Jan;118(1):87–101. [PMC free article] [PubMed]
  • Guo M, Birchler JA. Trans-acting dosage effects on the expression of model gene systems in maize aneuploids. Science. 1994 Dec 23;266(5193):1999–2002. [PubMed]
  • Kermicle JL. Androgenesis conditioned by a mutation in maize. Science. 1969 Dec 12;166(3911):1422–1424. [PubMed]
  • Lucchesi JC, Rawls JM., Jr Regulation of gene function: a comparison of enzyme activity levels in relation to gene dosage in diploids and triploids of Drosophila melanogaster. Biochem Genet. 1973 May;9(1):41–51. [PubMed]
  • Priest RE, Priest JH. Diploid and tetraploid clonal cells in culture: gene ploidy and synthesis of collagen. Biochem Genet. 1969 Aug;3(4):371–382. [PubMed]
  • Reichert GH. Two-dimensional gel analysis of proteins from mouse fetuses with trisomy 19 after DEAE-Sepharose chromatography. Genet Res. 1986 Jun;47(3):193–197. [PubMed]
  • Timko MP, Vasconcelos AC, Fairbrothers DE. Euploidy in Ricinus. I. Euploidy and gene dosage effects on cellular proteins. Biochem Genet. 1980 Feb;18(1-2):171–183. [PubMed]
  • Whatley SA, Hall C, Davison AN, Lim L. Alterations in the relative amounts of specific mRNA species in the developing human brain in Down's syndrome. Biochem J. 1984 May 15;220(1):179–187. [PMC free article] [PubMed]

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