• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Apr 1996; 142(4): 1249–1263.
PMCID: PMC1207122

The Complete Nucleotide Sequence of the Mitochondrial Genome of the Lungfish (Protopterus Dolloi) Supports Its Phylogenetic Position as a Close Relative of Land Vertebrates

Abstract

The complete DNA sequence (16,646 bp) of the mitochondrial genome of the African lungfish, Protopterus dolloi, was determined. The evolutionary position of lungfish as possibly the closest living relative among fish of land vertebrates made its mitochondrial DNA sequence particularly interesting. Its mitochondrial gene order conforms to the consensus vertebrate gene order. Several sequence motifs and secondary structures likely involved in the regulation of the initiation of replication and transcription of the mitochondrial genome are conserved in the lungfish and are more similar to those of land vertebrates than those of ray-finned fish. A novel feature discovered is that the putative origin of L-strand replication partially overlaps the adjacent tRNA(Cys). The phylogenetic analyses of genes coding for tRNAs and proteins confirm the intermediate phylogenetic position of lungfish between ray-finned fishes and tetrapods. The complete nucleotide sequence of the African lungfish mitochondrial genome was used to estimate which mitochondrial genes are most appropriate to elucidate deep branch phylogenies. Only a combined set of either protein or tRNA mitochondrial genes (but not each gene alone) is able to confidently recover the expected phylogeny among vertebrates that have diverged up to but not over ~400 mya.

Full Text

The Full Text of this article is available as a PDF (2.5M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, et al. Sequence and organization of the human mitochondrial genome. Nature. 1981 Apr 9;290(5806):457–465. [PubMed]
  • Anderson S, de Bruijn MH, Coulson AR, Eperon IC, Sanger F, Young IG. Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. J Mol Biol. 1982 Apr 25;156(4):683–717. [PubMed]
  • Arnason U, Gullberg A. Comparison between the complete mtDNA sequences of the blue and the fin whale, two species that can hybridize in nature. J Mol Evol. 1993 Oct;37(4):312–322. [PubMed]
  • Arnason U, Gullberg A, Johnsson E, Ledje C. The nucleotide sequence of the mitochondrial DNA molecule of the grey seal, Halichoerus grypus, and a comparison with mitochondrial sequences of other true seals. J Mol Evol. 1993 Oct;37(4):323–330. [PubMed]
  • Benton MJ. Phylogeny of the major tetrapod groups: morphological data and divergence dates. J Mol Evol. 1990 May;30(5):409–424. [PubMed]
  • Boore JL, Collins TM, Stanton D, Daehler LL, Brown WM. Deducing the pattern of arthropod phylogeny from mitochondrial DNA rearrangements. Nature. 1995 Jul 13;376(6536):163–165. [PubMed]
  • Brown WM, George M, Jr, Wilson AC. Rapid evolution of animal mitochondrial DNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1967–1971. [PMC free article] [PubMed]
  • Desjardins P, Morais R. Sequence and gene organization of the chicken mitochondrial genome. A novel gene order in higher vertebrates. J Mol Biol. 1990 Apr 20;212(4):599–634. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Dillon MC, Wright JM. Nucleotide sequence of the D-loop region of the sperm whale (Physeter macrocephalus) mitochondrial genome. Mol Biol Evol. 1993 Mar;10(2):296–305. [PubMed]
  • Doda JN, Wright CT, Clayton DA. Elongation of displacement-loop strands in human and mouse mitochondrial DNA is arrested near specific template sequences. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6116–6120. [PMC free article] [PubMed]
  • Hedges SB, Hass CA, Maxson LR. Relations of fish and tetrapods. Nature. 1993 Jun 10;363(6429):501–502. [PubMed]
  • Jama M, Zhang Y, Aman RA, Ryder OA. Sequence of the mitochondrial control region, tRNA(Thr), tRNA(Pro) and tRNA(Phe) genes from the black rhinoceros, Diceros bicornis. Nucleic Acids Res. 1993 Sep 11;21(18):4392–4392. [PMC free article] [PubMed]
  • Janke A, Feldmaier-Fuchs G, Thomas WK, von Haeseler A, Päbo S. The marsupial mitochondrial genome and the evolution of placental mammals. Genetics. 1994 May;137(1):243–256. [PMC free article] [PubMed]
  • Johansen S, Guddal PH, Johansen T. Organization of the mitochondrial genome of Atlantic cod, Gadus morhua. Nucleic Acids Res. 1990 Feb 11;18(3):411–419. [PMC free article] [PubMed]
  • Kishino H, Hasegawa M. Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. J Mol Evol. 1989 Aug;29(2):170–179. [PubMed]
  • Meyer A, Dolven SI. Molecules, fossils, and the origin of tetrapods. J Mol Evol. 1992 Aug;35(2):102–113. [PubMed]
  • Meyer A, Wilson AC. Origin of tetrapods inferred from their mitochondrial DNA affiliation to lungfish. J Mol Evol. 1990 Nov;31(5):359–364. [PubMed]
  • Saccone C, Pesole G, Sbisá E. The main regulatory region of mammalian mitochondrial DNA: structure-function model and evolutionary pattern. J Mol Evol. 1991 Jul;33(1):83–91. [PubMed]
  • Seutin G, Lang BF, Mindell DP, Morais R. Evolution of the WANCY region in amniote mitochondrial DNA. Mol Biol Evol. 1994 May;11(3):329–340. [PubMed]
  • Southern SO, Southern PJ, Dizon AE. Molecular characterization of a cloned dolphin mitochondrial genome. J Mol Evol. 1988 Dec;28(1-2):32–42. [PubMed]
  • Stewart DT, Baker AJ. Patterns of sequence variation in the mitochondrial D-loop region of shrews. Mol Biol Evol. 1994 Jan;11(1):9–21. [PubMed]
  • Tzeng CS, Hui CF, Shen SC, Huang PC. The complete nucleotide sequence of the Crossostoma lacustre mitochondrial genome: conservation and variations among vertebrates. Nucleic Acids Res. 1992 Sep 25;20(18):4853–4858. [PMC free article] [PubMed]
  • Walberg MW, Clayton DA. Sequence and properties of the human KB cell and mouse L cell D-loop regions of mitochondrial DNA. Nucleic Acids Res. 1981 Oct 24;9(20):5411–5421. [PMC free article] [PubMed]
  • Wilkinson GS, Chapman AM. Length and sequence variation in evening bat D-loop mtDNA. Genetics. 1991 Jul;128(3):607–617. [PMC free article] [PubMed]
  • Zardoya R, Villalta M, López-Pérez MJ, Garrido-Pertierra A, Montoya J, Bautista JM. Nucleotide sequence of the sheep mitochondrial DNA D-loop and its flanking tRNA genes. Curr Genet. 1995 Jun;28(1):94–96. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...