Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Mar 1996; 142(3): 987–1000.
PMCID: PMC1207035

RFLP Mapping in Cultivated Sugarcane (Saccharum Spp.): Genome Organization in a Highly Polyploid and Aneuploid Interspecific Hybrid


Sugarcane cultivars are polyploid, aneuploid, interspecific hybrids between the domesticated species Saccharum officinarum and the wild relative S. spontaneum. Cultivar chromosome numbers range from 100 to 130 with ~10% contributed by S. spontaneum. We have undertaken a mapping study on the progeny of a selfed cultivar, R570, to analyze this complex genome structure. A set of 128 restriction fragment length polymorphism probes and one isozyme was used. Four hundred and eight markers were placed onto 96 cosegregation groups, based on linkages in coupling only. These groups could tentatively be assembled into 10 basic linkage groups on the basis of common probes. Origin of markers was investigated for 61 probes and the isozyme, leading to the identification of 80 S. officinarum and 66 S. spontaneum derived markers, respectively. Their distribution in cosegregation groups showed better map coverage for the S. spontaneum than for the S. officinarum genome fraction and occasional recombination between the two genomes. The study of repulsions between markers suggested the prevalence of random pairing between chromosomes, typical of autopolyploids. However, cases of preferential pairing between S. spontaneum chromosomes were also detected. A tentative Saccharum map was constructed by pooling linkage information for each linkage group.

Full Text

The Full Text of this article is available as a PDF (1.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • al-Janabi SM, Honeycutt RJ, McClelland M, Sobral BW. A genetic linkage map of Saccharum spontaneum L. 'SES 208'. Genetics. 1993 Aug;134(4):1249–1260. [PMC free article] [PubMed]
  • Burr B, Burr FA. Recombinant inbreds for molecular mapping in maize: theoretical and practical considerations. Trends Genet. 1991 Feb;7(2):55–60. [PubMed]
  • Dvorák J, Chen KC. Distribution of Nonstructural Variation between Wheat Cultivars along Chromosome Arm 6Bp: Evidence from the Linkage Map and Physical Map of the Arm. Genetics. 1984 Feb;106(2):325–333. [PMC free article] [PubMed]
  • Gardiner JM, Coe EH, Melia-Hancock S, Hoisington DA, Chao S. Development of a core RFLP map in maize using an immortalized F2 population. Genetics. 1993 Jul;134(3):917–930. [PMC free article] [PubMed]
  • Gerlach WL, Bedbrook JR. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 1979 Dec 11;7(7):1869–1885. [PMC free article] [PubMed]
  • Helentjaris T, Weber D, Wright S. Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics. 1988 Feb;118(2):353–363. [PMC free article] [PubMed]
  • Kesseli RV, Paran I, Michelmore RW. Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. Genetics. 1994 Apr;136(4):1435–1446. [PMC free article] [PubMed]
  • Moore G, Foote T, Helentjaris T, Devos K, Kurata N, Gale M. Was there a single ancestral cereal chromosome? Trends Genet. 1995 Mar;11(3):81–82. [PubMed]
  • Vallejos CE, Sakiyama NS, Chase CD. A molecular marker-based linkage map of Phaseolus vulgaris L. Genetics. 1992 Jul;131(3):733–740. [PMC free article] [PubMed]
  • Whitkus R, Doebley J, Lee M. Comparative genome mapping of Sorghum and maize. Genetics. 1992 Dec;132(4):1119–1130. [PMC free article] [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...