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Genetics. Dec 1995; 141(4): 1245–1252.
PMCID: PMC1206863

Recombination between Chromosomal Is200 Elements Supports Frequent Duplication Formation in Salmonella Typhimurium


Spontaneous tandem chromosomal duplications are common in populations of Escherichia coli and Salmonella typhimurium. They range in frequency for a given locus from 10(-2) to 10(-4) and probably form by RecA-dependent unequal sister strand exchanges between repetitive sequences in direct order. Certain duplications have been observed previously to confer a growth advantage under specific selective conditions. Tandem chromosomal duplications are unstable and are lost at high frequencies, representing a readily reversible source of genomic variation. Six copies of a small mobile genetic element IS200 are evenly distributed around the chromosome of S. typhimurium strain LT2. A survey of 120 independent chromosomal duplications (20 for each of six loci) revealed that recombination between IS200 elements accounted for the majority of the duplications isolated for three of the loci tested. Duplications of the his operon were almost exclusively due to recombination between repeated IS200 elements. These data add further support to the idea that mobile genetic elements provide sequence repeats that play an important role in recombinational chromosome rearrangements, which may contribute to adaptation of bacteria to stressful conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson RP, Roth JR. Tandem genetic duplications in Salmonella typhimurium: amplification of the histidine operon. J Mol Biol. 1978 Nov 25;126(1):53–71. [PubMed]
  • Bisercić M, Ochman H. The ancestry of insertion sequences common to Escherichia coli and Salmonella typhimurium. J Bacteriol. 1993 Dec;175(24):7863–7868. [PMC free article] [PubMed]
  • Bisercić M, Ochman H. Natural populations of Escherichia coli and Salmonella typhimurium harbor the same classes of insertion sequences. Genetics. 1993 Mar;133(3):449–454. [PMC free article] [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PMC free article] [PubMed]
  • Gibert I, Barbé J, Casadesús J. Distribution of insertion sequence IS200 in Salmonella and Shigella. J Gen Microbiol. 1990 Dec;136(12):2555–2560. [PubMed]
  • Higgins CF, Ames GF, Barnes WM, Clement JM, Hofnung M. A novel intercistronic regulatory element of prokaryotic operons. Nature. 1982 Aug 19;298(5876):760–762. [PubMed]
  • Hill CW, Harnish BW. Inversions between ribosomal RNA genes of Escherichia coli. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7069–7072. [PMC free article] [PubMed]
  • Hill CW, Harnish BW. Transposition of a chromosomal segment bounded by redundant rRNA genes into other rRNA genes in Escherichia coli. J Bacteriol. 1982 Feb;149(2):449–457. [PMC free article] [PubMed]
  • Hughes KT, Roth JR. Transitory cis complementation: a method for providing transposition functions to defective transposons. Genetics. 1988 May;119(1):9–12. [PMC free article] [PubMed]
  • Lam S, Roth JR. Structural and functional studies of insertion element IS200. J Mol Biol. 1986 Jan 20;187(2):157–167. [PubMed]
  • Lin RJ, Capage M, Hill CW. A repetitive DNA sequence, rhs, responsible for duplications within the Escherichia coli K-12 chromosome. J Mol Biol. 1984 Jul 25;177(1):1–18. [PubMed]
  • Mekalanos JJ. Duplication and amplification of toxin genes in Vibrio cholerae. Cell. 1983 Nov;35(1):253–263. [PubMed]
  • Sanderson KE, Sciore P, Liu SL, Hessel A. Location of IS200 on the genomic cleavage map of Salmonella typhimurium LT2. J Bacteriol. 1993 Dec;175(23):7624–7628. [PMC free article] [PubMed]
  • Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972;119(1):75–88. [PubMed]
  • Shyamala V, Schneider E, Ames GF. Tandem chromosomal duplications: role of REP sequences in the recombination event at the join-point. EMBO J. 1990 Mar;9(3):939–946. [PMC free article] [PubMed]
  • Sonti RV, Roth JR. Role of gene duplications in the adaptation of Salmonella typhimurium to growth on limiting carbon sources. Genetics. 1989 Sep;123(1):19–28. [PMC free article] [PubMed]
  • Tsao SG, Brunk CF, Pearlman RE. Hybridization of nucleic acids directly in agarose gels. Anal Biochem. 1983 Jun;131(2):365–372. [PubMed]
  • VOGEL HJ, BONNER DM. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed]

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