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Genetics. Jun 1995; 140(2): 679–695.
PMCID: PMC1206644

Microsatellite Variation in Honey Bee (Apis Mellifera L.) Populations: Hierarchical Genetic Structure and Test of the Infinite Allele and Stepwise Mutation Models

Abstract

Samples from nine populations belonging to three African (intermissa, scutellata and capensis) and four European (mellifera, ligustica, carnica and cecropia) Apis mellifera subspecies were scored for seven microsatellite loci. A large amount of genetic variation (between seven and 30 alleles per locus) was detected. Average heterozygosity and average number of alleles were significantly higher in African than in European subspecies, in agreement with larger effective population sizes in Africa. Microsatellite analyses confirmed that A. mellifera evolved in three distinct and deeply differentiated lineages previously detected by morphological and mitochondrial DNA studies. Dendrogram analysis of workers from a given population indicated that super-sisters cluster together when using a sufficient number of microsatellite data whereas half-sisters do not. An index of classification was derived to summarize the clustering of different taxonomic levels in large phylogenetic trees based on individual genotypes. Finally, individual population X loci data were used to test the adequacy of the two alternative mutation models, the infinite allele model (IAM) and the stepwise mutation models. The better fit overall of the IAM probably results from the majority of the microsatellites used including repeats of two or three different length motifs (compound microsatellites).

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Selected References

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  • Bowcock AM, Ruiz-Linares A, Tomfohrde J, Minch E, Kidd JR, Cavalli-Sforza LL. High resolution of human evolutionary trees with polymorphic microsatellites. Nature. 1994 Mar 31;368(6470):455–457. [PubMed]
  • Callen DF, Thompson AD, Shen Y, Phillips HA, Richards RI, Mulley JC, Sutherland GR. Incidence and origin of "null" alleles in the (AC)n microsatellite markers. Am J Hum Genet. 1993 May;52(5):922–927. [PMC free article] [PubMed]
  • Chakraborty R, Jin L. A unified approach to study hypervariable polymorphisms: statistical considerations of determining relatedness and population distances. EXS. 1993;67:153–175. [PubMed]
  • Chakraborty R, Neel JV. Description and validation of a method for simultaneous estimation of effective population size and mutation rate from human population data. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9407–9411. [PMC free article] [PubMed]
  • Chakraborty R, Weiss KM. Genetic variation of the mitochondrial DNA genome in American Indians is at mutation-drift equilibrium. Am J Phys Anthropol. 1991 Dec;86(4):497–506. [PubMed]
  • Deka R, Chakroborty R, Ferrell RE. A population genetic study of six VNTR loci in three ethnically defined populations. Genomics. 1991 Sep;11(1):83–92. [PubMed]
  • Di Rienzo A, Peterson AC, Garza JC, Valdes AM, Slatkin M, Freimer NB. Mutational processes of simple-sequence repeat loci in human populations. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3166–3170. [PMC free article] [PubMed]
  • Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R. Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics. 1992 Feb;12(2):241–253. [PubMed]
  • Estoup A, Solignac M, Harry M, Cornuet JM. Characterization of (GT)n and (CT)n microsatellites in two insect species: Apis mellifera and Bombus terrestris. Nucleic Acids Res. 1993 Mar 25;21(6):1427–1431. [PMC free article] [PubMed]
  • Ewens WJ. The sampling theory of selectively neutral alleles. Theor Popul Biol. 1972 Mar;3(1):87–112. [PubMed]
  • Hunt J. Application of a pressure area risk calculator in an intensive care unit. Intensive Crit Care Nurs. 1993 Dec;9(4):226–231. [PubMed]
  • Garnery L, Cornuet JM, Solignac M. Evolutionary history of the honey bee Apis mellifera inferred from mitochondrial DNA analysis. Mol Ecol. 1992 Oct;1(3):145–154. [PubMed]
  • Gottelli D, Sillero-Zubiri C, Applebaum GD, Roy MS, Girman DJ, Garcia-Moreno J, Ostrander EA, Wayne RK. Molecular genetics of the most endangered canid: the Ethiopian wolf Canis simensis. Mol Ecol. 1994 Aug;3(4):301–312. [PubMed]
  • Hedges SB. The number of replications needed for accurate estimation of the bootstrap P value in phylogenetic studies. Mol Biol Evol. 1992 Mar;9(2):366–369. [PubMed]
  • Hunt GJ, Page RE., Jr Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics. 1995 Mar;139(3):1371–1382. [PMC free article] [PubMed]
  • Kimura M, Ohta T. Stepwise mutation model and distribution of allelic frequencies in a finite population. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2868–2872. [PMC free article] [PubMed]
  • Moran PA. Wandering distributions and the electrophoretic profile. II. Theor Popul Biol. 1976 Oct;10(2):145–149. [PubMed]
  • Ohta T, Kimura M. A model of mutation appropriate to estimate the number of electrophoretically detectable alleles in a finite population. Genet Res. 1973 Oct;22(2):201–204. [PubMed]
  • Pamilo P, Crozier RH. Genic Variation in Male Haploids under Deterministic Selection. Genetics. 1981 May;98(1):199–214. [PMC free article] [PubMed]
  • Shriver MD, Jin L, Chakraborty R, Boerwinkle E. VNTR allele frequency distributions under the stepwise mutation model: a computer simulation approach. Genetics. 1993 Jul;134(3):983–993. [PMC free article] [PubMed]
  • Tarès S, Cornuet JM, Abad P. Characterization of an unusually conserved AluI highly reiterated DNA sequence family from the honeybee, Apis mellifera. Genetics. 1993 Aug;134(4):1195–1204. [PMC free article] [PubMed]
  • Taylor AC, Sherwin WB, Wayne RK. Genetic variation of microsatellite loci in a bottlenecked species: the northern hairy-nosed wombat Lasiorhinus krefftii. Mol Ecol. 1994 Aug;3(4):277–290. [PubMed]
  • Valdes AM, Slatkin M, Freimer NB. Allele frequencies at microsatellite loci: the stepwise mutation model revisited. Genetics. 1993 Mar;133(3):737–749. [PMC free article] [PubMed]

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