• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Mar 1995; 139(3): 1371–1382.
PMCID: PMC1206463

Linkage Map of the Honey Bee, Apis Mellifera, Based on Rapd Markers

Abstract

A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be ~3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.

Full Text

The Full Text of this article is available as a PDF (2.1M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Carlier M, Roubertoux PL, Kottler ML, Degrelle H. Y chromosome and aggression in strains of laboratory mice. Behav Genet. 1990 Jan;20(1):137–156. [PubMed]
  • Contel EP, Mestriner MA, Martins E. Genetic control and development expression of malate dehydrogenase in Apis mellifera. Biochem Genet. 1977 Oct;15(9-10):859–876. [PubMed]
  • Crain WR, Davidson EH, Britten RJ. Contrasting patterns of DNA sequence arrangement in Apis mellifera (honeybee) and Musca domestica (housefly). Chromosoma. 1976 Dec 6;59(1):1–12. [PubMed]
  • de Belle JS, Hilliker AJ, Sokolowski MB. Genetic localization of foraging (for): a major gene for larval behavior in Drosophila melanogaster. Genetics. 1989 Sep;123(1):157–163. [PMC free article] [PubMed]
  • Dietrich W, Katz H, Lincoln SE, Shin HS, Friedman J, Dracopoli NC, Lander ES. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics. 1992 Jun;131(2):423–447. [PMC free article] [PubMed]
  • Gage LP. The Bombyx mori genome: analysis by DNA reassociation kinetics. Chromosoma. 1974 Mar 1;45(1):27–42. [PubMed]
  • Jordan RA, Brosemer RW. Characterization of DNA from three bee species. J Insect Physiol. 1974 Dec;20(12):2513–2520. [PubMed]
  • Jost E, Mameli M. DNA content in nine species of Nematocera with special reference to the sibling species of the Anopheles maculipennis group and the Culex pipiens group. Chromosoma. 1972;37(2):201–208. [PubMed]
  • Kafatos FC, Louis C, Savakis C, Glover DM, Ashburner M, Link AJ, Sidén-Kiamos I, Saunders RD. Integrated maps of the Drosophila genome: progress and prospects. Trends Genet. 1991 May;7(5):155–161. [PubMed]
  • King JS, Mortimer RK. A polymerization model of chiasma interference and corresponding computer simulation. Genetics. 1990 Dec;126(4):1127–1138. [PMC free article] [PubMed]
  • Laird CD. DNA of Drosophila chromosomes. Annu Rev Genet. 1973;7:177–204. [PubMed]
  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. [PubMed]
  • Lincoln SE, Lander ES. Systematic detection of errors in genetic linkage data. Genomics. 1992 Nov;14(3):604–610. [PubMed]
  • Metcalf RA, Marlin JC, Whitt GS. Low levels of genetic heterozygosity in hymenoptera. Nature. 1975 Oct 30;257(5529):792–794. [PubMed]
  • Mortimer RK, Contopoulou CR, King JS. Genetic and physical maps of Saccharomyces cerevisiae, Edition 11. Yeast. 1992 Oct;8(10):817–902. [PubMed]
  • Morton NE. Parameters of the human genome. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7474–7476. [PMC free article] [PubMed]
  • Olson M, Hood L, Cantor C, Botstein D. A common language for physical mapping of the human genome. Science. 1989 Sep 29;245(4925):1434–1435. [PubMed]
  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD. Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature. 1988 Oct 20;335(6192):721–726. [PubMed]
  • Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD. Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics. 1991 Jan;127(1):181–197. [PMC free article] [PubMed]
  • Rao PN, Rai K. Inter and intraspecific variation in nuclear DNA content in Aedes mosquitoes. Heredity (Edinb) 1987 Oct;59(Pt 2):253–258. [PubMed]
  • Rasch EM, Cassidy JD, King RC. Evidence for dosage compensation in parthenogenetic Hymenoptera. Chromosoma. 1977 Feb 23;59(4):323–340. [PubMed]
  • Severson DW, Mori A, Zhang Y, Christensen BM. Linkage map for Aedes aegypti using restriction fragment length polymorphisms. J Hered. 1993 Jul-Aug;84(4):241–247. [PubMed]
  • Sparrow AH, Price HJ, Underbrink AG. A survey of DNA content per cell and per chromosome of prokaryotic and eukaryotic organisms: some evolutionary considerations. Brookhaven Symp Biol. 1972;23:451–494. [PubMed]
  • Stuber CW, Lincoln SE, Wolff DW, Helentjaris T, Lander ES. Identification of genetic factors contributing to heterosis in a hybrid from two elite maize inbred lines using molecular markers. Genetics. 1992 Nov;132(3):823–839. [PMC free article] [PubMed]
  • Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, et al. High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992 Dec;132(4):1141–1160. [PMC free article] [PubMed]
  • Tzeng TH, Lyngholm LK, Ford CF, Bronson CR. A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus. Genetics. 1992 Jan;130(1):81–96. [PMC free article] [PubMed]
  • Wells R, Royer HD, Hollenberg CP. Non Xenopus-like DNA sequence organization in the Chironomus tentans genome. Mol Gen Genet. 1976 Aug 10;147(1):45–51. [PubMed]
  • WHITING AR. Genetics of Habrobracon. Adv Genet. 1961;10:295–348. [PubMed]
  • Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. [PMC free article] [PubMed]
  • Zheng L, Saunders RD, Fortini D, della Torre A, Coluzzi M, Glover DM, Kafatos FC. Low-resolution genome map of the malaria mosquito Anopheles gambiae. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11187–11191. [PMC free article] [PubMed]
  • Zheng L, Collins FH, Kumar V, Kafatos FC. A detailed genetic map for the X chromosome of the malaria vector, Anopheles gambiae. Science. 1993 Jul 30;261(5121):605–608. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

    Your browsing activity is empty.

    Activity recording is turned off.

    Turn recording back on

    See more...