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Genetics. Aug 1994; 137(4): 1121–1137.
PMCID: PMC1206059

Genetic Linkage Maps of Eucalyptus Grandis and Eucalyptus Urophylla Using a Pseudo-Testcross: Mapping Strategy and Rapd Markers

Abstract

We have used a ``two-way pseudo-testcross'' mapping strategy in combination with the random amplified polymorhic DNA (RAPD) assay to construct two moderate density genetic linkage maps for species of Eucalyptus. In the cross between two heterozygous individuals many single-dose RAPD markers will be heterozygous in one parent, null in the other and therefore segregate 1:1 in their F(1) progeny following a testcross configuration. Meiosis and gametic segregation in each individual can be directly and efficiently analyzed using RAPD markers. We screened 305 primers of arbitrary sequence, and selected 151 to amplify a total of 558 markers. These markers were grouped at LOD 5.0, θ = 0.25, resulting in the maternal Eucalyptus grandis map having a total of 240 markers into 14 linkage groups (1552 cM) and the paternal Eucalyptus urophylla map with 251 markers in 11 linkage groups (1101 cM) (n = 11 in Eucalyptus). Framework maps ordered with a likelihood support >/=1000:1 were assembled covering 95% of the estimated genome size in both individuals. Characterization of genome complexity of a sample of 48 mapped random amplified polymorphic DNA (RAPD) markers indicate that 53% amplify from low copy regions. These are the first reported high coverage linkage maps for any species of Eucalyptus and among the first for any hardwood tree species. We propose the combined use of RAPD markers and the pseudo-testcross configuration as a general strategy for the construction of single individual genetic linkage maps in outbred forest trees as well as in any highly heterozygous sexually reproducing living organism. A survey of the occurrence of RAPD markers in different individuals suggests that the pseudo-testcross/RAPD mapping strategy should also be efficient at the intraspecific level and increasingly so with crosses of genetically divergent individuals. The ability to quickly construct single-tree genetic linkage maps in any forest species opens the way for a shift from the paradigm of a species index map to the heterodox proposal of constructing several maps for individual trees of a population, therefore mitigating the problem of linkage equilibrium between marker and trait loci for the application of marker assisted strategies in tree breeding.

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Selected References

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  • al-Janabi SM, Honeycutt RJ, McClelland M, Sobral BW. A genetic linkage map of Saccharum spontaneum L. 'SES 208'. Genetics. 1993 Aug;134(4):1249–1260. [PMC free article] [PubMed]
  • Bonierbale MW, Plaisted RL, Tanksley SD. RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato. Genetics. 1988 Dec;120(4):1095–1103. [PMC free article] [PubMed]
  • Caetano-Anollés G, Bassam BJ, Gresshoff PM. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology (N Y) 1991 Jun;9(6):553–557. [PubMed]
  • Darvasi A, Weinreb A, Minke V, Weller JI, Soller M. Detecting marker-QTL linkage and estimating QTL gene effect and map location using a saturated genetic map. Genetics. 1993 Jul;134(3):943–951. [PMC free article] [PubMed]
  • Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD. Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res. 1991 Dec 11;19(23):6553–6558. [PMC free article] [PubMed]
  • Hulbert SH, Ilott TW, Legg EJ, Lincoln SE, Lander ES, Michelmore RW. Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics. 1988 Dec;120(4):947–958. [PMC free article] [PubMed]
  • Kazan K, Manners JM, Cameron DF. Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes. Genome. 1993 Feb;36(1):50–56. [PubMed]
  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. [PubMed]
  • Morgante M, Olivieri AM. PCR-amplified microsatellites as markers in plant genetics. Plant J. 1993 Jan;3(1):175–182. [PubMed]
  • Wilkinson SP, Williams R. Renin-angiotensin-aldosterone system in cirrhosis. Gut. 1980 Jun;21(6):545–554. [PMC free article] [PubMed]
  • Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R. Correlation of genetic and physical structure in the region surrounding the I2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet. 1992 Jan;231(2):179–185. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, et al. High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992 Dec;132(4):1141–1160. [PMC free article] [PubMed]
  • Tulsieram LK, Glaubitz JC, Kiss G, Carlson JE. Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes. Biotechnology (N Y) 1992 Jun;10(6):686–690. [PubMed]
  • Vallejos CE, Sakiyama NS, Chase CD. A molecular marker-based linkage map of Phaseolus vulgaris L. Genetics. 1992 Jul;131(3):733–740. [PMC free article] [PubMed]
  • Weissenbach J, Gyapay G, Dib C, Vignal A, Morissette J, Millasseau P, Vaysseix G, Lathrop M. A second-generation linkage map of the human genome. Nature. 1992 Oct 29;359(6398):794–801. [PubMed]
  • Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. [PMC free article] [PubMed]
  • Williams JG, Hanafey MK, Rafalski JA, Tingey SV. Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol. 1993;218:704–740. [PubMed]
  • Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. [PMC free article] [PubMed]

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