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Genetics. Aug 1994; 137(4): 1019–1026.
PMCID: PMC1206049

Meiotic Gene Conversion Tract Length Distribution within the Rosy Locus of Drosophila Melanogaster

Abstract

Employing extensive co-conversion data for selected and unselected sites of known molecular location in the rosy locus of Drosophila melanogaster, we determine the parameters of meiotic gene conversion tract length distribution. The tract length distribution for gene conversion events can be approximated by the equation P(L >/= n) = [var phi](n) where P is the probability that tract length (L) is greater than or equal to a specified number of nucleotides (n). From the co-conversion data, a maximum likelihood estimate with standard error for [var phi] is 0.99717 +/- 0.00026, corresponding to a mean conversion tract length of 352 base pairs. (Thus, gene conversion tract lengths are sufficiently small to allow for extensive shuffling of DNA sequence polymorphisms within a gene.) For selected site conversions there is a bias towards recovery of longer tracts. The distribution of conversion tract lengths associated with selected sites can be approximated by the equation P(L >/= n| selected = [var phi](n)(1 - n + n/[var phi]), where P is now the probability that a selected site tract length (L) is greater than or equal to a specified number of nucleotides (n). For the optimal value of [var phi] determined from the co-conversion analysis, the mean conversion tract length for selected sites is 706 base pairs. We discuss, in the light of this and other studies, the relationship between meiotic gene conversion and P element excision induced gap repair and determine that they are distinct processes defined by different parameters and, possibly, mechanisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Banga SS, Velazquez A, Boyd JB. P transposition in Drosophila provides a new tool for analyzing postreplication repair and double-strand break repair. Mutat Res. 1991 Jul;255(1):79–88. [PubMed]
  • Carpenter AT. Mismatch repair, gene conversion, and crossing-over in two recombination-defective mutants of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5961–5965. [PMC free article] [PubMed]
  • Carpenter AT. Meiotic roles of crossing-over and of gene conversion. Cold Spring Harb Symp Quant Biol. 1984;49:23–29. [PubMed]
  • Chovnick A, Ballantyne GH, Baillie DL, Holm DG. Gene conversion in higher organisms: half-tetrad analysis of recombination within the rosy cistron of Drosophila melanogaster. Genetics. 1970 Oct;66(2):315–329. [PMC free article] [PubMed]
  • Chovnick A, Ballantyne GH, Holm DG. Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics. 1971 Oct;69(2):179–209. [PMC free article] [PubMed]
  • Clark SH, Daniels S, Rushlow CA, Hilliker AJ, Chovnick A. Tissue-specific and pretranslational character of variants of the rosy locus control element in Drosophila melanogaster. Genetics. 1984 Dec;108(4):953–968. [PMC free article] [PubMed]
  • Clark SH, Hilliker AJ, Chovnick A. Recombination can initiate and terminate at a large number of sites within the rosy locus of Drosophila melanogaster. Genetics. 1988 Feb;118(2):261–266. [PMC free article] [PubMed]
  • Curtis D, Clark SH, Chovnick A, Bender W. Molecular analysis of recombination events in Drosophila. Genetics. 1989 Jul;122(3):653–661. [PMC free article] [PubMed]
  • Dutton FL, Jr, Chovnick A. Developmental regulation of the rosy locus in Drosophila melanogaster. Dev Biol (N Y 1985) 1988;5:267–316. [PubMed]
  • Engels WR, Johnson-Schlitz DM, Eggleston WB, Sved J. High-frequency P element loss in Drosophila is homolog dependent. Cell. 1990 Aug 10;62(3):515–525. [PubMed]
  • Gloor GB, Nassif NA, Johnson-Schlitz DM, Preston CR, Engels WR. Targeted gene replacement in Drosophila via P element-induced gap repair. Science. 1991 Sep 6;253(5024):1110–1117. [PubMed]
  • Gray M, Charpentier A, Walsh K, Wu P, Bender W. Mapping point mutations in the Drosophila rosy locus using denaturing gradient gel blots. Genetics. 1991 Jan;127(1):139–149. [PMC free article] [PubMed]
  • Hilliker AJ, Clark SH, Chovnick A. The effect of DNA sequence polymorphisms on intragenic recombination in the rosy locus of Drosophila melanogaster. Genetics. 1991 Nov;129(3):779–781. [PMC free article] [PubMed]
  • Johnson-Schlitz DM, Engels WR. P-element-induced interallelic gene conversion of insertions and deletions in Drosophila melanogaster. Mol Cell Biol. 1993 Nov;13(11):7006–7018. [PMC free article] [PubMed]
  • Lee CS, Curtis D, McCarron M, Love C, Gray M, Bender W, Chovnick A. Mutations affecting expression of the rosy locus in Drosophila melanogaster. Genetics. 1987 May;116(1):55–66. [PMC free article] [PubMed]
  • McCarron M, O'Donnell J, Chovnick A, Bhullar BS, Hewitt J, Candido EP. Organization of the rosy locus in Drosophila melanogaster: further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element. Genetics. 1979 Feb;91(2):275–293. [PMC free article] [PubMed]
  • Nassif N, Engels W. DNA homology requirements for mitotic gap repair in Drosophila. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1262–1266. [PMC free article] [PubMed]
  • Smith PD, Finnerty VG, Chovnick A. Gene conversion in Drosophila: non-reciprocal events at the maroon-like cistron. Nature. 1970 Oct 31;228(5270):442–444. [PubMed]

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