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Genetics. Nov 1993; 135(3): 869–879.
PMCID: PMC1205726

Unusual Mitochondrial Genome Organization in Cytoplasmic Male Sterile Common Bean and the Nature of Cytoplasmic Reversion to Fertility

Abstract

Spontaneous reversion to pollen fertility and fertility restoration by the nuclear gene Fr in cytoplasmic male sterile common bean (Phaseolus vulgaris L.) are associated with the loss of a large portion of the mitochondrial genome. To understand better the molecular events responsible for this DNA loss, we have constructed a physical map of the mitochondrial genome of a stable fertile revertant line, WPR-3, and the cytoplasmic male sterile line (CMS-Sprite) from which it was derived. This involved a cosmid clone walking strategy with comparative DNA gel blot hybridizations. Mapping data suggested that the simplest model for the structure of the CMS-Sprite genome consists of three autonomous chromosomes differing only in short, unique regions. The unique region contained on one of these chromosomes is the male sterility-associated 3-kb sequence designated pvs. Based on genomic environments surrounding repeated sequences, we predict that chromosomes can undergo intra- and intermolecular recombination. The mitochondrial genome of the revertant line appeared to contain only two of the three chromosomes; the region containing the pvs sequence was absent. Therefore, the process of spontaneous cytoplasmic reversion to fertility likely involves the disappearance of an entire mitochondrial chromosome. This model is supported by the fact that we detected no evidence of recombination, excision or deletion events within the revertant genome that could account for the loss of a large segment of mitochondrial DNA.

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Selected References

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  • André C, Levy A, Walbot V. Small repeated sequences and the structure of plant mitochondrial genomes. Trends Genet. 1992 Apr;8(4):128–132. [PubMed]
  • Azpiroz R, Butow RA. Patterns of mitochondrial sorting in yeast zygotes. Mol Biol Cell. 1993 Jan;4(1):21–36. [PMC free article] [PubMed]
  • Bland MM, Levings CS, 3rd, Matzinger DF. The tobacco mitochondrial ATPase subunit 9 gene is closely linked to an open reading frame for a ribosomal protein. Mol Gen Genet. 1986 Jul;204(1):8–16. [PubMed]
  • Chase CD, Ortega VM. Organization of ATPA coding and 3' flanking sequences associated with cytoplasmic male sterility in Phaseolus vulgaris L. Curr Genet. 1992 Aug;22(2):147–153. [PubMed]
  • Chen XJ, Clark-Walker GD. Mutations in MGI genes convert Kluyveromyces lactis into a petite-positive yeast. Genetics. 1993 Mar;133(3):517–525. [PMC free article] [PubMed]
  • Dale RM, Duesing JH, Keene D. Supercoiled mitochondrial DNAs from plant tissue culture cells. Nucleic Acids Res. 1981 Sep 25;9(18):4583–4593. [PMC free article] [PubMed]
  • Diffley JF, Stillman B. A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7864–7868. [PMC free article] [PubMed]
  • Fauron CM, Havlik M, Brettell RI. The mitochondrial genome organization of a maize fertile cmsT revertant line is generated through recombination between two sets of repeats. Genetics. 1990 Feb;124(2):423–428. [PMC free article] [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Hanson MR. Plant mitochondrial mutations and male sterility. Annu Rev Genet. 1991;25:461–486. [PubMed]
  • Johns C, Lu M, Lyznik A, Mackenzie S. A mitochondrial DNA sequence is associated with abnormal pollen development in cytoplasmic male sterile bean plants. Plant Cell. 1992 Apr;4(4):435–449. [PMC free article] [PubMed]
  • Kawano S, Takano H, Imai J, Mori K, Kurioiwa T. A genetic system controlling mitochondrial fusion in the slime mould, Physarum polycephalum. Genetics. 1993 Feb;133(2):213–224. [PMC free article] [PubMed]
  • Kolodner R, Tewari KK. Physicochemical characterization of mitochondrial DNA from pea leaves. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1830–1834. [PMC free article] [PubMed]
  • Lonsdale DM, Hodge TP, Fauron CM. The physical map and organisation of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res. 1984 Dec 21;12(24):9249–9261. [PMC free article] [PubMed]
  • Mackenzie SA. Identification of a sterility-inducing cytoplasm in a fertile accession line of Phaseolus vulgaris L. Genetics. 1991 Feb;127(2):411–416. [PMC free article] [PubMed]
  • Mackenzie SA, Chase CD. Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean. Plant Cell. 1990 Sep;2(9):905–912. [PMC free article] [PubMed]
  • McConnell SJ, Stewart LC, Talin A, Yaffe MP. Temperature-sensitive yeast mutants defective in mitochondrial inheritance. J Cell Biol. 1990 Sep;111(3):967–976. [PMC free article] [PubMed]
  • Narayanan KK, André CP, Yang J, Walbot V. Organization of a 117-kb circular mitochondrial chromosome in IR36 rice. Curr Genet. 1993 Mar;23(3):248–254. [PubMed]
  • Synenki RM, Levings CS, Shah DM. Physicochemical characterization of mitochondrial DNA from soybean. Plant Physiol. 1978 Mar;61(3):460–464. [PMC free article] [PubMed]
  • Zweifel SG, Fangman WL. A nuclear mutation reversing a biased transmission of yeast mitochondrial DNA. Genetics. 1991 Jun;128(2):241–249. [PMC free article] [PubMed]

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