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Genetics. Jul 1993; 134(3): 917–930.
PMCID: PMC1205526

Development of a Core RFLP Map in Maize Using an Immortalized F(2) Population

Abstract

A map derived from restriction fragment length polymorphisms (RFLPs) in maize (Zea mays L.) is presented. The map was constructed in an immortalized Tx303 X CO159 F(2) mapping population that allowed for an unlimited number of markers to be mapped and pooled F(3) seed to be distributed to other laboratories. A total of 215 markers consisting of 159 genomic clones, 16 isozymes and 35 cloned genes of defined function have been placed on 10 chromosomes. An examination of segregation data has revealed several genomic regions with aberrant segregation ratios favoring either parent or the heterozygote. Mapping of cloned genes and isozymes that have been previously mapped by functional criteria has provided 29 points of alignment with the classical maize genetic map. Screening of all mapped RFLP probes against a collection of U.S. Corn Belt germplasm using EcoRI, HindIII and EcoRV has resulted in a set of 97 core markers being defined. The designation of a set of core markers allows the maize genome to be subdivided into a series of bins which serve as the backbone for maize genetic information and database boundaries. The merits and applications of core markers and bins are discussed.

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Selected References

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  • Belanger FC, Kriz AL. Molecular characterization of the major maize embryo globulin encoded by the glb1 gene. Plant Physiol. 1989 Oct;91(2):636–643. [PMC free article] [PubMed]
  • Bernatzky R, Tanksley SD. Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics. 1986 Apr;112(4):887–898. [PMC free article] [PubMed]
  • Birnboim HC. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 1983;100:243–255. [PubMed]
  • Bonierbale MW, Plaisted RL, Tanksley SD. RFLP Maps Based on a Common Set of Clones Reveal Modes of Chromosomal Evolution in Potato and Tomato. Genetics. 1988 Dec;120(4):1095–1103. [PMC free article] [PubMed]
  • Brinkmann H, Martinez P, Quigley F, Martin W, Cerff R. Endosymbiotic origin and codon bias of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize. J Mol Evol. 1987;26(4):320–328. [PubMed]
  • Burr B, Burr FA. Ds controlling elements of maize at the shrunken locus are large and dissimilar insertions. Cell. 1982 Jul;29(3):977–986. [PubMed]
  • Burr B, Burr FA. Recombinant inbreds for molecular mapping in maize: theoretical and practical considerations. Trends Genet. 1991 Feb;7(2):55–60. [PubMed]
  • Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW. Gene mapping with recombinant inbreds in maize. Genetics. 1988 Mar;118(3):519–526. [PMC free article] [PubMed]
  • Burr B, Burr FA, Matz EC, Romero-Severson J. Pinning down loose ends: mapping telomeres and factors affecting their length. Plant Cell. 1992 Aug;4(8):953–960. [PMC free article] [PubMed]
  • Chang C, Bowman JL, DeJohn AW, Lander ES, Meyerowitz EM. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856–6860. [PMC free article] [PubMed]
  • Cone KC, Burr FA, Burr B. Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9631–9635. [PMC free article] [PubMed]
  • Dennis ES, Sachs MM, Gerlach WL, Finnegan EJ, Peacock WJ. Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize. Nucleic Acids Res. 1985 Feb 11;13(3):727–743. [PMC free article] [PubMed]
  • Di Fonzo N, Hartings H, Brembilla M, Motto M, Soave C, Navarro E, Palau J, Rhode W, Salamini F. The b-32 protein from maize endosperm, an albumin regulated by the O2 locus: nucleic acid (cDNA) and amino acid sequences. Mol Gen Genet. 1988 Jun;212(3):481–487. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD. Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res. 1991 Dec 11;19(23):6553–6558. [PMC free article] [PubMed]
  • Gowri G, Campbell WH. cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD(P):Glyceraldehyde-3-Phosphate Dehydrogenase : Characterization of the Clones and Analysis of the Expression of the Genes in Leaves as Influenced by Nitrate in the Light and Dark. Plant Physiol. 1989 Jul;90(3):792–798. [PMC free article] [PubMed]
  • Haldane JB, Waddington CH. Inbreeding and Linkage. Genetics. 1931 Jul;16(4):357–374. [PMC free article] [PubMed]
  • Helentjaris T, Weber DF, Wright S. Use of monosomics to map cloned DNA fragments in maize. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6035–6039. [PMC free article] [PubMed]
  • Helentjaris T, Weber D, Wright S. Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics. 1988 Feb;118(2):353–363. [PMC free article] [PubMed]
  • Hudspeth RL, Glackin CA, Bonner J, Grula JW. Genomic and cDNA clones for maize phosphoenolpyruvate carboxylase and pyruvate,orthophosphate dikinase: Expression of different gene-family members in leaves and roots. Proc Natl Acad Sci U S A. 1986 May;83(9):2884–2888. [PMC free article] [PubMed]
  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. [PubMed]
  • Lechelt C, Peterson T, Laird A, Chen J, Dellaporta SL, Dennis E, Peacock WJ, Starlinger P. Isolation and molecular analysis of the maize P locus. Mol Gen Genet. 1989 Oct;219(1-2):225–234. [PubMed]
  • Mayfield SP, Taylor WC. Carotenoid-deficient maize seedlings fail to accumulate light-harvesting chlorophyll a/b binding protein (LHCP) mRNA. Eur J Biochem. 1984 Oct 1;144(1):79–84. [PubMed]
  • Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828–9832. [PMC free article] [PubMed]
  • Nam HG, Giraudat J, Den Boer B, Moonan F, Loos WDB, Hauge BM, Goodman HM. Restriction Fragment Length Polymorphism Linkage Map of Arabidopsis thaliana. Plant Cell. 1989 Jul;1(7):699–705. [PMC free article] [PubMed]
  • Nieto-Sotelo J, Vierling E, Ho TH. Cloning, sequence analysis, and expression of a cDNA encoding a plastid-localized heat shock protein in maize. Plant Physiol. 1990 Aug;93(4):1321–1328. [PMC free article] [PubMed]
  • O'Reilly C, Shepherd NS, Pereira A, Schwarz-Sommer Z, Bertram I, Robertson DS, Peterson PA, Saedler H. Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1. EMBO J. 1985 Apr;4(4):877–882. [PMC free article] [PubMed]
  • Rochester DE, Winer JA, Shah DM. The structure and expression of maize genes encoding the major heat shock protein, hsp70. EMBO J. 1986 Mar;5(3):451–458. [PMC free article] [PubMed]
  • Russell DA, Sachs MM. Differential expression and sequence analysis of the maize glyceraldehyde-3-phosphate dehydrogenase gene family. Plant Cell. 1989 Aug;1(8):793–803. [PMC free article] [PubMed]
  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW. Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci U S A. 1984 Dec;81(24):8014–8018. [PMC free article] [PubMed]
  • Shin JS, Chao S, Corpuz L, Blake T. A partial map of the barley genome incorporating restriction fragment length polymorphism, polymerase chain reaction, isozyme, and morphological marker loci. Genome. 1990 Dec;33(6):803–810. [PubMed]
  • Shure M, Wessler S, Fedoroff N. Molecular identification and isolation of the Waxy locus in maize. Cell. 1983 Nov;35(1):225–233. [PubMed]
  • Smith RD, Walker JC. Isolation and expression of a maize type 1 protein phosphatase. Plant Physiol. 1991 Oct;97(2):677–683. [PMC free article] [PubMed]
  • Weber D, Helentjaris T. Mapping RFLP loci in maize using B-A translocations. Genetics. 1989 Mar;121(3):583–590. [PMC free article] [PubMed]
  • Wendel JF, Edwards MD, Stuber CW. Evidence for multilocus genetic control of preferential fertilisation in maize. Heredity (Edinb) 1987 Apr;58(Pt 2):297–301. [PubMed]
  • Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. [PMC free article] [PubMed]

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