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Genetics. Jun 1992; 131(2): 423–447.
PMCID: PMC1205016

A Genetic Map of the Mouse Suitable for Typing Intraspecific Crosses


We report the construction of a genetic linkage map of the mouse, consisting entirely of genetic markers that can be rapidly typed by polymerase chain reaction and that show a high degree of polymorphism among inbred laboratory strains. Specifically, the map contains 317 simple sequence length polymorphisms at an average spacing of 4.3 cM and is detectably linked to approximately 99% of the mouse genome. In typical crosses between inbred laboratory strains, about 50% of the markers are polymorphic, making it straightforward to follow inheritance in almost any cross.

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Selected References

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  • Atchley WR, Fitch WM. Gene trees and the origins of inbred strains of mice. Science. 1991 Oct 25;254(5031):554–558. [PubMed]
  • Avner P, Amar L, Dandolo L, Guénet JL. Genetic analysis of the mouse using interspecific crosses. Trends Genet. 1988 Jan;4(1):18–23. [PubMed]
  • Bahary N, Zorich G, Pachter JE, Leibel RL, Friedman JM. Molecular genetic linkage maps of mouse chromosomes 4 and 6. Genomics. 1991 Sep;11(1):33–47. [PubMed]
  • Bailey DW. Recombinant-inbred strains. An aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation. 1971 Mar;11(3):325–327. [PubMed]
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980 May;32(3):314–331. [PMC free article] [PubMed]
  • Breslauer KJ, Frank R, Blöcker H, Marky LA. Predicting DNA duplex stability from the base sequence. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3746–3750. [PMC free article] [PubMed]
  • Buchberg AM, Brownell E, Nagata S, Jenkins NA, Copeland NG. A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human. Genetics. 1989 May;122(1):153–161. [PMC free article] [PubMed]
  • Buetow KH. Influence of aberrant observations on high-resolution linkage analysis outcomes. Am J Hum Genet. 1991 Nov;49(5):985–994. [PMC free article] [PubMed]
  • Ceci JD, Siracusa LD, Jenkins NA, Copeland NG. A molecular genetic linkage map of mouse chromosome 4 including the localization of several proto-oncogenes. Genomics. 1989 Nov;5(4):699–709. [PubMed]
  • Ceci JD, Justice MJ, Lock LF, Jenkins NA, Copeland NG. An interspecific backcross linkage map of mouse chromosome 8. Genomics. 1990 Jan;6(1):72–79. [PubMed]
  • Chen YH, Pentecost BT, McLachlan JA, Teng CT. The androgen-dependent mouse seminal vesicle secretory protein IV: characterization and complementary deoxyribonucleic acid cloning. Mol Endocrinol. 1987 Oct;1(10):707–716. [PubMed]
  • Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. [PMC free article] [PubMed]
  • Copeland NG, Jenkins NA. Development and applications of a molecular genetic linkage map of the mouse genome. Trends Genet. 1991 Apr;7(4):113–118. [PubMed]
  • Cornall RJ, Aitman TJ, Hearne CM, Todd JA. The generation of a library of PCR-analyzed microsatellite variants for genetic mapping of the mouse genome. Genomics. 1991 Aug;10(4):874–881. [PubMed]
  • Frankel WN, Stoye JP, Taylor BA, Coffin JM. A linkage map of endogenous murine leukemia proviruses. Genetics. 1990 Feb;124(2):221–236. [PMC free article] [PubMed]
  • Hamada H, Petrino MG, Kakunaga T. A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6465–6469. [PMC free article] [PubMed]
  • Hearne CM, McAleer MA, Love JM, Aitman TJ, Cornall RJ, Ghosh S, Knight AM, Prins JB, Todd JA. Additional microsatellite markers for mouse genome mapping. Mamm Genome. 1991;1(4):273–282. [PubMed]
  • Hilbert P, Lindpaintner K, Beckmann JS, Serikawa T, Soubrier F, Dubay C, Cartwright P, De Gouyon B, Julier C, Takahasi S, et al. Chromosomal mapping of two genetic loci associated with blood-pressure regulation in hereditary hypertensive rats. Nature. 1991 Oct 10;353(6344):521–529. [PubMed]
  • Howard TE, Shai SY, Langford KG, Martin BM, Bernstein KE. Transcription of testicular angiotensin-converting enzyme (ACE) is initiated within the 12th intron of the somatic ACE gene. Mol Cell Biol. 1990 Aug;10(8):4294–4302. [PMC free article] [PubMed]
  • Jacks CM, Powaser CB, Hackett PB. Sequence analysis of a processed gene coding for mouse ribosomal protein L32. Gene. 1988 Dec 30;74(2):565–570. [PubMed]
  • Jacob HJ, Lindpaintner K, Lincoln SE, Kusumi K, Bunker RK, Mao YP, Ganten D, Dzau VJ, Lander ES. Genetic mapping of a gene causing hypertension in the stroke-prone spontaneously hypertensive rat. Cell. 1991 Oct 4;67(1):213–224. [PubMed]
  • Justice MJ, Siracusa LD, Gilbert DJ, Heisterkamp N, Groffen J, Chada K, Silan CM, Copeland NG, Jenkins NA. A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross. Genetics. 1990 Aug;125(4):855–866. [PMC free article] [PubMed]
  • Kingsley DM, Jenkins NA, Copeland NG. A molecular genetic linkage map of mouse chromosome 9 with regional localizations for the Gsta, T3g, Ets-1 and Ldlr loci. Genetics. 1989 Sep;123(1):165–172. [PMC free article] [PubMed]
  • Krayev AS, Kramerov DA, Skryabin KG, Ryskov AP, Bayev AA, Georgiev GP. The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA. Nucleic Acids Res. 1980 Mar 25;8(6):1201–1215. [PMC free article] [PubMed]
  • Krayev AS, Markusheva TV, Kramerov DA, Ryskov AP, Skryabin KG, Bayev AA, Georgiev GP. Ubiquitous transposon-like repeats B1 and B2 of the mouse genome: B2 sequencing. Nucleic Acids Res. 1982 Dec 11;10(23):7461–7475. [PMC free article] [PubMed]
  • Loeb DD, Padgett RW, Hardies SC, Shehee WR, Comer MB, Edgell MH, Hutchison CA., 3rd The sequence of a large L1Md element reveals a tandemly repeated 5' end and several features found in retrotransposons. Mol Cell Biol. 1986 Jan;6(1):168–182. [PMC free article] [PubMed]
  • Love JM, Knight AM, McAleer MA, Todd JA. Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites. Nucleic Acids Res. 1990 Jul 25;18(14):4123–4130. [PMC free article] [PubMed]
  • Morry MJ, Harding JD. Modulation of transcriptional activity and stable complex formation by 5'-flanking regions of mouse tRNAHis genes. Mol Cell Biol. 1986 Jan;6(1):105–115. [PMC free article] [PubMed]
  • Oyler GA, Polli JW, Wilson MC, Billingsley ML. Developmental expression of the 25-kDa synaptosomal-associated protein (SNAP-25) in rat brain. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5247–5251. [PMC free article] [PubMed]
  • Robert B, Barton P, Minty A, Daubas P, Weydert A, Bonhomme F, Catalan J, Chazottes D, Guénet JL, Buckingham M. Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature. 1985 Mar 14;314(6007):181–183. [PubMed]
  • Rychlik W, Rhoads RE. A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA. Nucleic Acids Res. 1989 Nov 11;17(21):8543–8551. [PMC free article] [PubMed]
  • Schäfer R, Böltz E, Becker A, Bartels F, Epplen JT. The expression of the evolutionarily conserved GATA/GACA repeats in mouse tissues. Chromosoma. 1986;93(6):496–501. [PubMed]
  • Stahl J, Gearing DP, Willson TA, Brown MA, King JA, Gough NM. Structural organization of the genes for murine and human leukemia inhibitory factor. Evolutionary conservation of coding and non-coding regions. J Biol Chem. 1990 May 25;265(15):8833–8841. [PubMed]
  • Stallings RL, Ford AF, Nelson D, Torney DC, Hildebrand CE, Moyzis RK. Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics. 1991 Jul;10(3):807–815. [PubMed]
  • Taylor BA, Heiniger HJ, Meier H. Genetic analysis of resistance to cadmium-induced testicular damage in mice. Proc Soc Exp Biol Med. 1973 Jul;143(3):629–633. [PubMed]
  • Weber JL. Informativeness of human (dC-dA)n.(dG-dT)n polymorphisms. Genomics. 1990 Aug;7(4):524–530. [PubMed]

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