• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Dec 1991; 129(4): 1085–1098.
PMCID: PMC1204773

Chromosome Rearrangement by Ectopic Recombination in Drosophila Melanogaster: Genome Structure and Evolution

Abstract

Ectopic recombination between interspersed repeat sequences generates chromosomal rearrangements that have a major impact on genome structure. A survey of ectopic recombination in the region flanking the white locus of Drosophila melanogaster identified 25 transposon-mediated rearrangements from four parallel experiments. Eighteen of the 25 were generated from females carrying X chromosomes heterozygous for interspersed repeat sequences. The cytogenetic and molecular analyses of the rearrangements and the parental chromosomes show: (1) interchromosomal and intrachromosomal recombinants are generated in about equal numbers; (2) ectopic recombination appears to be a meiotic process that is stimulated by the interchromosomal effect to about the same degree as regular crossing over; (3) copies of the retrotransposon roo were involved in all of the interchromosomal exchanges; some copies were involved much more frequently than others in the target region; (4) homozygosis for interspersed repeat sequences and other sequence variations significantly reduced ectopic recombination.

Full Text

The Full Text of this article is available as a PDF (6.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bingham PM, Levis R, Rubin GM. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. [PubMed]
  • Charlesworth B, Langley CH. The evolution of self-regulated transposition of transposable elements. Genetics. 1986 Feb;112(2):359–383. [PMC free article] [PubMed]
  • Charlesworth B, Langley CH. The population genetics of Drosophila transposable elements. Annu Rev Genet. 1989;23:251–287. [PubMed]
  • Charlesworth B, Lapid A. A study of ten families of transposable elements on X chromosomes from a population of Drosophila melanogaster. Genet Res. 1989 Oct;54(2):113–125. [PubMed]
  • Davis PS, Shen MW, Judd BH. Asymmetrical pairings of transposons in and proximal to the white locus of Drosophila account for four classes of regularly occurring exchange products. Proc Natl Acad Sci U S A. 1987 Jan;84(1):174–178. [PMC free article] [PubMed]
  • Engels WR, Preston CR. Formation of chromosome rearrangements by P factors in Drosophila. Genetics. 1984 Aug;107(4):657–678. [PMC free article] [PubMed]
  • Finnegan DJ, Rubin GM, Young MW, Hogness DS. Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1053–1063. [PubMed]
  • Goldberg ML, Sheen JY, Gehring WJ, Green MM. Unequal crossing-over associated with asymmetrical synapsis between nomadic elements in the Drosophila melanogaster genome. Proc Natl Acad Sci U S A. 1983 Aug;80(16):5017–5021. [PMC free article] [PubMed]
  • Haber JE, Leung WY, Borts RH, Lichten M. The frequency of meiotic recombination in yeast is independent of the number and position of homologous donor sequences: implications for chromosome pairing. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1120–1124. [PMC free article] [PubMed]
  • Hawley RS. Chromosomal sites necessary for normal levels of meiotic recombination in Drosophila melanogaster. I. Evidence for and mapping of the sites. Genetics. 1980 Mar;94(3):625–646. [PMC free article] [PubMed]
  • Johnson-Schlitz D, Lim JK. Cytogenetics of Notch mutations arising in the unstable X chromosome Uc of Drosophila melanogaster. Genetics. 1987 Apr;115(4):701–709. [PMC free article] [PubMed]
  • Judd BH. Studies on Some Position Pseudoalleles at the White Region in Drosophila Melanogaster. Genetics. 1959 Jan;44(1):34–42. [PMC free article] [PubMed]
  • Judd BH. The white locus of Drosophila melanogaster. Results Probl Cell Differ. 1987;14:81–94. [PubMed]
  • Judd BH, Shen MW, Kaufman TC. The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics. 1972 May;71(1):139–156. [PMC free article] [PubMed]
  • Langer PR, Waldrop AA, Ward DC. Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633–6637. [PMC free article] [PubMed]
  • Langley CH, Montgomery E, Hudson R, Kaplan N, Charlesworth B. On the role of unequal exchange in the containment of transposable element copy number. Genet Res. 1988 Dec;52(3):223–235. [PubMed]
  • Lehrman MA, Goldstein JL, Russell DW, Brown MS. Duplication of seven exons in LDL receptor gene caused by Alu-Alu recombination in a subject with familial hypercholesterolemia. Cell. 1987 Mar 13;48(5):827–835. [PubMed]
  • Levis R, Bingham PM, Rubin GM. Physical map of the white locus of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1982 Jan;79(2):564–568. [PMC free article] [PubMed]
  • Lim JK. Intrachromosomal rearrangements mediated by hobo transposons in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9153–9157. [PMC free article] [PubMed]
  • Meyerowitz EM, Hogness DS. Molecular organization of a Drosophila puff site that responds to ecdysone. Cell. 1982 Jan;28(1):165–176. [PubMed]
  • Montgomery E, Charlesworth B, Langley CH. A test for the role of natural selection in the stabilization of transposable element copy number in a population of Drosophila melanogaster. Genet Res. 1987 Feb;49(1):31–41. [PubMed]
  • Montgomery EA, Langley CH. Transposable Elements in Mendelian Populations. II. Distribution of Three COPIA-like Elements in a Natural Population of DROSOPHILA MELANOGASTER. Genetics. 1983 Jul;104(3):473–483. [PMC free article] [PubMed]
  • Pardue ML, Gall JG. Nucleic acid hybridization to the DNA of cytological preparations. Methods Cell Biol. 1975;10:1–16. [PubMed]
  • Petes TD, Hill CW. Recombination between repeated genes in microorganisms. Annu Rev Genet. 1988;22:147–168. [PubMed]
  • Shrimpton AE, Montgomery EA, Langley CH. OM Mutations in DROSOPHILA ANANASSAE Are Linked to Insertions of a Transposable Element. Genetics. 1986 Sep;114(1):125–135. [PMC free article] [PubMed]
  • Stoppa-Lyonnet D, Carter PE, Meo T, Tosi M. Clusters of intragenic Alu repeats predispose the human C1 inhibitor locus to deleterious rearrangements. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1551–1555. [PMC free article] [PubMed]
  • Yen PH, Li XM, Tsai SP, Johnson C, Mohandas T, Shapiro LJ. Frequent deletions of the human X chromosome distal short arm result from recombination between low copy repetitive elements. Cell. 1990 May 18;61(4):603–610. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...