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Genetics. Oct 1991; 129(2): 489–499.
PMCID: PMC1204638

Associations between DNA Sequence Variation and Variation in Expression of the Adh Gene in Natural Populations of Drosophila Melanogaster


A large part of the genetic variation in alcohol dehydrogenase (ADH) activity level in natural populations of Drosophila melanogaster is associated with segregation of an amino acid replacement polymorphism at nucleotide 1490, which generates a difference in electrophoretic mobility. Part of the allozymic difference in activity level is due to a catalytic efficiency difference, which is also caused by the amino acid replacement, and part is due to a difference in the concentration of ADH protein. A previous site-directed in vitro mutagenesis experiment clearly demonstrated that the amino acid replacement has no effect on the concentration of ADH protein, nor does a strongly associated silent polymorphism at nucleotide 1443. Here we analyze associations between polymorphisms within the Adh gene and variation in ADH protein level for a number of chromosomes derived from natural populations. A sequence length polymorphism within the first intron of the distal (adult) transcript, &1, is in strong linkage disequilibrium with the amino acid replacement. Among a sample of 46 isochromosomal lines analyzed, all but one of the 14 Fast lines have &1 and all but one of the 32 Slow lines lack &1. The exceptional Fast line has an unusually low level of ADH protein (typical of Slow lines) and the exceptional Slow line has an unusually high level (typical of Fast lines). These results suggest that the &1 polymorphism may be responsible for the average difference in ADH protein between the allozymic classes. A previous experiment localized the effect on ADH protein to a 2.3-kb restriction fragment. DNA sequences of this fragment from several alleles of each allozymic type indicate that no other polymorphisms within this region are as closely associated with the ADH protein level difference as the &1 polymorphism.

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Selected References

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  • Anderson SM, McDonald JF. Biochemical and molecular analysis of naturally occurring Adh variants in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4798–4802. [PMC free article] [PubMed]
  • Buchman AR, Berg P. Comparison of intron-dependent and intron-independent gene expression. Mol Cell Biol. 1988 Oct;8(10):4395–4405. [PMC free article] [PubMed]
  • Choudhary M, Laurie CC. Use of in vitro mutagenesis to analyze the molecular basis of the difference in Adh expression associated with the allozyme polymorphism in Drosophila melanogaster. Genetics. 1991 Oct;129(2):481–488. [PMC free article] [PubMed]
  • Chovnick A, McCarron M, Clark SH, Hilliker AJ, Rushlow CA. Structural and functional organization of a gene in Drosophila melanogaster. Basic Life Sci. 1980;16:3–23. [PubMed]
  • Corbin V, Maniatis T. The role of specific enhancer-promoter interactions in the Drosophila Adh promoter switch. Genes Dev. 1989 Dec;3(12B):2191–2120. [PubMed]
  • Corbin V, Maniatis T. Role of transcriptional interference in the Drosophila melanogaster Adh promoter switch. Nature. 1989 Jan 19;337(6204):279–282. [PubMed]
  • Corbin V, Maniatis T. Identification of cis-regulatory elements required for larval expression of the Drosophila melanogaster alcohol dehydrogenase gene. Genetics. 1990 Mar;124(3):637–646. [PMC free article] [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Dickinson WJ. Genetic control of enzyme expression in Drosophila: a locus influencing tissue specificity of aldehyde oxidase. J Exp Zool. 1978 Dec;206(3):333–341. [PubMed]
  • Fischer JA, Maniatis T. Regulatory elements involved in Drosophila Adh gene expression are conserved in divergent species and separate elements mediate expression in different tissues. EMBO J. 1986 Jun;5(6):1275–1289. [PMC free article] [PubMed]
  • Frischauf AM, Murray N, Lehrach H. Lambda phage vectors--EMBL series. Methods Enzymol. 1987;153:103–115. [PubMed]
  • Game AY, Oakeshott JG. Associations between restriction site polymorphism and enzyme activity variation for esterase 6 in Drosophila melanogaster. Genetics. 1990 Dec;126(4):1021–1031. [PMC free article] [PubMed]
  • Higuchi RG, Ochman H. Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction. Nucleic Acids Res. 1989 Jul 25;17(14):5865–5865. [PMC free article] [PubMed]
  • Kreitman M. Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster. Nature. 1983 Aug 4;304(5925):412–417. [PubMed]
  • Langley CH, Shrimpton AE, Yamazaki T, Miyashita N, Matsuo Y, Aquadro CF. Naturally occurring variation in the restriction map of the amy region of Drosophila melanogaster. Genetics. 1988 Jul;119(3):619–629. [PMC free article] [PubMed]
  • Laurie CC, Heath EM, Jacobson JW, Thomson MS. Genetic basis of the difference in alcohol dehydrogenase expression between Drosophila melanogaster and Drosophila simulans. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9674–9678. [PMC free article] [PubMed]
  • Laurie-Ahlberg CC. Genetic variation affecting the expression of enzyme-coding genes in Drosophila: an evolutionary perspective. Isozymes Curr Top Biol Med Res. 1985;12:33–88. [PubMed]
  • Laurie-Ahlberg CC, Stam LF. Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. Genetics. 1987 Jan;115(1):129–140. [PMC free article] [PubMed]
  • Laurie-Ahlberg CC, Maroni G, Bewley GC, Lucchesi JC, Weir BS. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. [PMC free article] [PubMed]
  • Maroni G, Laurie-Ahlberg CC, Adams DA, Wilton AN. Genetic variation in the expression of ADH in Drosophila melanogaster. Genetics. 1982 Jul-Aug;101(3-4):431–446. [PMC free article] [PubMed]
  • Miyashita NT. Molecular and phenotypic variation of the Zw locus region in Drosophila melanogaster. Genetics. 1990 Jun;125(2):407–419. [PMC free article] [PubMed]
  • Munroe D, Jacobson A. Tales of poly(A): a review. Gene. 1990 Jul 16;91(2):151–158. [PubMed]
  • Posakony JW, Fischer JA, Maniatis T. Identification of DNA sequences required for the regulation of Drosophila alcohol dehydrogenase gene expression. Cold Spring Harb Symp Quant Biol. 1985;50:515–520. [PubMed]
  • Thomson MS, Jacobson JW, Laurie CC. Comparison of alcohol dehydrogenase expression in Drosophila melanogaster and D. simulans. Mol Biol Evol. 1991 Jan;8(1):31–48. [PubMed]

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