• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Sep 1991; 129(1): 169–175.
PMCID: PMC1204563

The Ecdysone-Induced Puffing Cascade in Drosophila Salivary Glands: A Broad-Complex Early Gene Regulates Intermolt and Late Gene Transcription


The steroid hormone 20-OH ecdysone triggers a classic and well-defined program of chromosome puffing that is assumed to reflect changes in transcriptional activity in Drosophila salivary glands. Mutations in each of four Broad-Complex locus (BR-C) complementation groups were analyzed for their effects on the expression of other genes that reside in several major salivary gland puffs. RNA blot analysis showed that the rbp function of the BR-C is required for the transcription of six genes in the 71E late puff and is the first demonstration that an ecdysone-induced early gene controls the transcription of late genes within the puffing cascade. In addition, the rbp function is required for the transcription of four intermolt genes (Sgs-3, Sgs-4, Sgs-5 and 71E gene VII). Mutations in the broad, l(1)2Bc and l(1)2Bd functions of the BR-C had no effect on the expression of the genes examined. We propose that the BR-C functions to control transcription at many other salivary gland loci at the beginning of metamorphosis.

Full Text

The Full Text of this article is available as a PDF (3.5M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ashburner M. Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. II. The effects of inhibitors of protein synthesis. Dev Biol. 1974 Jul;39(1):141–157. [PubMed]
  • Ashburner M, Chihara C, Meltzer P, Richards G. Temporal control of puffing activity in polytene chromosomes. Cold Spring Harb Symp Quant Biol. 1974;38:655–662. [PubMed]
  • Bainbridge SP, Bownes M. Staging the metamorphosis of Drosophila melanogaster. J Embryol Exp Morphol. 1981 Dec;66:57–80. [PubMed]
  • Beaton AH, Kiss I, Fristrom D, Fristrom JW. Interaction of the Stubble-stubbloid locus and the Broad-complex of Drosophila melanogaster. Genetics. 1988 Oct;120(2):453–464. [PMC free article] [PubMed]
  • Belyaeva ES, Aizenzon MG, Semeshin VF, Kiss II, Koczka K, Baritcheva EM, Gorelova TD, Zhimulev IF. Cytogenetic analysis of the 2B3-4--2B11 region of the X-chromosome of Drosophila melanogaster. I. Cytology of the region and mutant complementation groups. Chromosoma. 1980;81(2):281–306. [PubMed]
  • Belyaeva ES, Vlassova IE, Biyasheva ZM, Kakpakov VT, Richards G, Zhimulev IF. Cytogenetic analysis of the 2B3-4-2B11 region of the X chromosome of Drosophila melanogaster. II. Changes in 20-OH ecdysone puffing caused by genetic defects of puff 2B5. Chromosoma. 1981;84(2):207–219. [PubMed]
  • Burtis KC, Thummel CS, Jones CW, Karim FD, Hogness DS. The Drosophila 74EF early puff contains E74, a complex ecdysone-inducible gene that encodes two ets-related proteins. Cell. 1990 Apr 6;61(1):85–99. [PubMed]
  • Chao AT, Guild GM. Molecular analysis of the ecdysterone-inducible 2B5 "early' puff in Drosophila melanogaster. EMBO J. 1986 Jan;5(1):143–150. [PMC free article] [PubMed]
  • Georgel P, Ramain P, Giangrande A, Dretzen G, Richards G, Bellard M. Sgs-3 chromatin structure and trans-activators: developmental and ecdysone induction of a glue enhancer-binding factor, GEBF-I, in Drosophila larvae. Mol Cell Biol. 1991 Jan;11(1):523–532. [PMC free article] [PubMed]
  • Guild GM. Molecular analysis of a developmentally regulated gene which is expressed in the larval salivary gland of Drosophila. Dev Biol. 1984 Apr;102(2):462–470. [PubMed]
  • Harrison SD, Travers AA. The tramtrack gene encodes a Drosophila finger protein that interacts with the ftz transcriptional regulatory region and shows a novel embryonic expression pattern. EMBO J. 1990 Jan;9(1):207–216. [PMC free article] [PubMed]
  • McNabb SL, Beckendorf SK. Cis-acting sequences which regulate expression of the Sgs-4 glue protein gene of Drosophila. EMBO J. 1986 Sep;5(9):2331–2340. [PMC free article] [PubMed]
  • Meyerowitz EM, Hogness DS. Molecular organization of a Drosophila puff site that responds to ecdysone. Cell. 1982 Jan;28(1):165–176. [PubMed]
  • Muskavitch MA, Hogness DS. Molecular analysis of a gene in a developmentally regulated puff of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7362–7366. [PMC free article] [PubMed]
  • Restifo LL, Guild GM. An ecdysterone-responsive puff site in Drosophila contains a cluster of seven differentially regulated genes. J Mol Biol. 1986 Apr 20;188(4):517–528. [PubMed]
  • Restifo LL, Guild GM. Poly(A) shortening of coregulated transcripts in Drosophila. Dev Biol. 1986 Jun;115(2):507–510. [PubMed]
  • Segraves WA, Hogness DS. The E75 ecdysone-inducible gene responsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily. Genes Dev. 1990 Feb;4(2):204–219. [PubMed]
  • Semeshin VF, Baricheva EM, Belyaeva ES, Zhimulev IF. Electron microscopical analysis of Drosophila polytene chromosomes. II. Development of complex puffs. Chromosoma. 1985;91(3-4):210–233. [PubMed]
  • Shore EM, Guild GM. Larval salivary gland secretion proteins in Drosophila structural analysis of the Sgs-5 gene. J Mol Biol. 1986 Jul 20;190(2):149–158. [PubMed]
  • Shore EM, Guild GM. Closely linked DNA elements control the expression of the Sgs-5 glue protein gene in Drosophila. Genes Dev. 1987 Oct;1(8):829–839. [PubMed]
  • Thummel CS, Burtis KC, Hogness DS. Spatial and temporal patterns of E74 transcription during Drosophila development. Cell. 1990 Apr 6;61(1):101–111. [PubMed]
  • Urness LD, Thummel CS. Molecular interactions within the ecdysone regulatory hierarchy: DNA binding properties of the Drosophila ecdysone-inducible E74A protein. Cell. 1990 Oct 5;63(1):47–61. [PubMed]
  • Vigoreaux JO, Tobin SL. Stage-specific selection of alternative transcriptional initiation sites from the 5C actin gene of Drosophila melanogaster. Genes Dev. 1987 Dec;1(10):1161–1171. [PubMed]
  • Walker VK, Ashburner M. The control of ecdysterone-regulated puffs in Drosophila salivary glands. Cell. 1981 Oct;26(2 Pt 2):269–277. [PubMed]
  • Zhimulev IF, Vlassova IE, Belyaeva ES. Cytogenetic analysis of the 2B3-4--2B11 region of the X chromosome of Drosophila melanogaster. III. Puffing disturbance in salivary gland chromosomes of homozygotes for mutation l(1)pp1t10. Chromosoma. 1982;85(5):659–672. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...