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Genetics. Nov 1990; 126(3): 695–711.
PMCID: PMC1204224

Phylogenetically Informative Length Polymorphism and Sequence Variability in Mitochondrial DNA of Australian Songbirds (Pomatostomus)


A combination of restriction analysis and direct sequencing via the polymerase chain reaction (PCR) was used to build trees relating mitochondrial DNAs (mtDNAs) from 50 individuals belonging to five species of Australian babblers (Pomatostomus). The trees served as a quantitative framework for analyzing the direction and tempo of evolution of an intraspecific length polymorphism from a third mitochondrial ancestor. The length polymorphism lies between the cytochrome b and 12S rRNA (srRNA) genes. Screening of mtDNAs within and between the five species with restriction enzymes showed that Pomatosomus temporalis was polymorphic for two smaller size classes (M and S) that are completely segregated geographically, whereas mtDNAs from the other four species were exclusively of a third, larger size (L). Inter- and intraspecific phylogenetic trees relating mtDNAs based on restriction maps, cytochrome b sequences obtained via PCR, and the two data sets combined were compared to one another statistically and were broadly similar except for the phylogenetic position of Pomatosomus halli. Both sets of phylogenies imply that only two deletion events can account for the observed intraspecific distribution of the three length types. High levels of base-substitutional divergence were detected within and between northern and southern lineages of P. temporalis, which implies a low level of gene flow between northern and southern regions as well as a low rate of length mutation. These conclusions were confirmed by applying coalescent theory to the statistical framework provided by the phylogenetic analyses.

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Selected References

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  • Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, et al. Sequence and organization of the human mitochondrial genome. Nature. 1981 Apr 9;290(5806):457–465. [PubMed]
  • Ball RM, Freeman S, James FC, Bermingham E, Avise JC. Phylogeographic population structure of Red-winged Blackbirds assessed by mitochondrial DNA. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1558–1562. [PMC free article] [PubMed]
  • Bentzen P, Leggett WC, Brown GG. Length and restriction site heteroplasmy in the mitochondrial DNA of american shad (alosa sapidissima). Genetics. 1988 Mar;118(3):509–518. [PMC free article] [PubMed]
  • Bermingham E, Lamb T, Avise JC. Size polymorphism and heteroplasmy in the mitochondrial DNA of lower vertebrates. J Hered. 1986 Jul-Aug;77(4):249–252. [PubMed]
  • Brown WM. Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3605–3609. [PMC free article] [PubMed]
  • Brown WM, Prager EM, Wang A, Wilson AC. Mitochondrial DNA sequences of primates: tempo and mode of evolution. J Mol Evol. 1982;18(4):225–239. [PubMed]
  • Buroker NE, Brown JR, Gilbert TA, O'Hara PJ, Beckenbach AT, Thomas WK, Smith MJ. Length heteroplasmy of sturgeon mitochondrial DNA: an illegitimate elongation model. Genetics. 1990 Jan;124(1):157–163. [PMC free article] [PubMed]
  • Cabot EL, Beckenbach AT. Simultaneous editing of multiple nucleic acid and protein sequences with ESEE. Comput Appl Biosci. 1989 Jul;5(3):233–234. [PubMed]
  • Cann RL, Wilson AC. Length mutations in human mitochondrial DNA. Genetics. 1983 Aug;104(4):699–711. [PMC free article] [PubMed]
  • Densmore LD, Wright JW, Brown WM. Length variation and heteroplasmy are frequent in mitochondrial DNA from parthenogenetic and bisexual lizards (genus Cnemidophorus). Genetics. 1985 Aug;110(4):689–707. [PMC free article] [PubMed]
  • Desjardins P, Morais R. Sequence and gene organization of the chicken mitochondrial genome. A novel gene order in higher vertebrates. J Mol Biol. 1990 Apr 20;212(4):599–634. [PubMed]
  • Gyllensten UB, Erlich HA. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652–7656. [PMC free article] [PubMed]
  • Harrison RG, Rand DM, Wheeler WC. Mitochondrial DNA size variation within individual crickets. Science. 1985 Jun 21;228(4706):1446–1448. [PubMed]
  • Hixson JE, Brown WM. A comparison of the small ribosomal RNA genes from the mitochondrial DNA of the great apes and humans: sequence, structure, evolution, and phylogenetic implications. Mol Biol Evol. 1986 Jan;3(1):1–18. [PubMed]
  • Kan YW, Dozy AM. Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5631–5635. [PMC free article] [PubMed]
  • Kocher TD, Thomas WK, Meyer A, Edwards SV, Päbo S, Villablanca FX, Wilson AC. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6196–6200. [PMC free article] [PubMed]
  • Moritz C, Brown WM. Tandem duplication of D-loop and ribosomal RNA sequences in lizard mitochondrial DNA. Science. 1986 Sep 26;233(4771):1425–1427. [PubMed]
  • Moritz C, Brown WM. Tandem duplications in animal mitochondrial DNAs: variation in incidence and gene content among lizards. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7183–7187. [PMC free article] [PubMed]
  • Prager EM, Wilson AC. Ancient origin of lactalbumin from lysozyme: analysis of DNA and amino acid sequences. J Mol Evol. 1988;27(4):326–335. [PubMed]
  • Rand DM, Harrison RG. Molecular population genetics of mtDNA size variation in crickets. Genetics. 1989 Mar;121(3):551–569. [PMC free article] [PubMed]
  • Shields GF, Wilson AC. Calibration of mitochondrial DNA evolution in geese. J Mol Evol. 1987;24(3):212–217. [PubMed]
  • Slatkin M. Detecting small amounts of gene flow from phylogenies of alleles. Genetics. 1989 Mar;121(3):609–612. [PMC free article] [PubMed]
  • Solignac M, Monnerot M, Mounolou JC. Mitochondrial DNA heteroplasmy in Drosophila mauritiana. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6942–6946. [PMC free article] [PubMed]
  • Takahata N. Gene genealogy in three related populations: consistency probability between gene and population trees. Genetics. 1989 Aug;122(4):957–966. [PMC free article] [PubMed]
  • Thomas RH, Schaffner W, Wilson AC, Päbo S. DNA phylogeny of the extinct marsupial wolf. Nature. 1989 Aug 10;340(6233):465–467. [PubMed]
  • Thomas WK, Maa J, Wilson AC. Shifting constraints on tRNA genes during mitochondrial DNA evolution in animals. New Biol. 1989 Oct;1(1):93–100. [PubMed]
  • Thomas WK, Päbo S, Villablanca FX, Wilson AC. Spatial and temporal continuity of kangaroo rat populations shown by sequencing mitochondrial DNA from museum specimens. J Mol Evol. 1990 Aug;31(2):101–112. [PubMed]
  • Vigilant L, Stoneking M, Wilson AC. Conformational mutation in human mtDNA detected by direct sequencing of enzymatically amplified DNA. Nucleic Acids Res. 1988 Jul 11;16(13):5945–5955. [PMC free article] [PubMed]
  • Vigilant L, Pennington R, Harpending H, Kocher TD, Wilson AC. Mitochondrial DNA sequences in single hairs from a southern African population. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9350–9354. [PMC free article] [PubMed]

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