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Genetics. May 1989; 122(1): 139–151.
PMCID: PMC1203678

Co-Localization to Chromosome Bands 99e1-3 of the Drosophila Melanogaster Myosin Light Chain-2 Gene and a Haplo-Insufficient Locus That Affects Flight Behavior


Using overlapping synthetic deficiencies, we find that a haplo-insufficient locus affecting flight behavior and the myosin light chain-2 gene co-map to the Drosophila melanogaster polytene chromosome interval 99D9-E1 to 99E2-3. From screening over 9000 EMS-treated chromosomes, we obtained alleles of two complementation groups that map to this same interval. One of these complementation groups, Ifm(3)99Eb, exhibits dominant flightless behavior; thus, flightless behavior of the deficiency is in all likelihood due to hemizygosity of this single locus. Rescue of flightless behavior by a duplication indicates that the single allele, E38, of the Ifm(3)99Eb complementation group is a hypomorph. Based upon its map position and a reduction in concentration of myosin light chain-2 mRNA in heterozygotes, we propose that Ifm(3)99Eb(E38) is a mutant allele of the myosin light chain-2 gene. Our genetic analysis also resulted in the identification of four dominant flightless alleles of an unlinked locus, l(3)nc99Eb, that exhibits dominant lethal synergism with Ifm(3)99Eb.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Benzer S. Genetic dissection of behavior. Sci Am. 1973 Dec;229(6):24–37. [PubMed]
  • Bonner JJ, Pardue ML. Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Assay by in situ hybridization. Chromosoma. 1976 Oct 12;58(1):87–99. [PubMed]
  • Endow SA, Gall JG. Differential replication of satellite DNA in polyploid tissues of Drosophila virilis. Chromosoma. 1975;50(2):175–179. [PubMed]
  • Falkenthal S, Parker VP, Mattox WW, Davidson N. Drosophila melanogaster has only one myosin alkali light-chain gene which encodes a protein with considerable amino acid sequence homology to chicken myosin alkali light chains. Mol Cell Biol. 1984 May;4(5):956–965. [PMC free article] [PubMed]
  • Frisardi MC, MacIntyre RJ. Position effect variegation of an acid phosphatase gene in Drosophila melanogaster. Mol Gen Genet. 1984;197(3):403–413. [PubMed]
  • Hiromi Y, Hotta Y. Actin gene mutations in Drosophila; heat shock activation in the indirect flight muscles. EMBO J. 1985 Jul;4(7):1681–1687. [PMC free article] [PubMed]
  • Homyk T, Jr, Emerson CP., Jr Functional interactions between unlinked muscle genes within haploinsufficient regions of the Drosophila genome. Genetics. 1988 May;119(1):105–121. [PMC free article] [PubMed]
  • Karlik CC, Coutu MD, Fyrberg EA. A nonsense mutation within the act88F actin gene disrupts myofibril formation in Drosophila indirect flight muscles. Cell. 1984 Oct;38(3):711–719. [PubMed]
  • Karlik CC, Mahaffey JW, Coutu MD, Fyrberg EA. Organization of contractile protein genes within the 88F subdivision of the D. melanogaster third chromosome. Cell. 1984 Jun;37(2):469–481. [PubMed]
  • Karlik CC, Fyrberg EA. An insertion within a variably spliced Drosophila tropomyosin gene blocks accumulation of only one encoded isoform. Cell. 1985 May;41(1):57–66. [PubMed]
  • Karlik CC, Saville DL, Fyrberg EA. Two missense alleles of the Drosophila melanogaster act88F actin gene are strongly antimorphic but only weakly induce synthesis of heat shock proteins. Mol Cell Biol. 1987 Sep;7(9):3084–3091. [PMC free article] [PubMed]
  • Kidd S, Lockett TJ, Young MW. The Notch locus of Drosophila melanogaster. Cell. 1983 Sep;34(2):421–433. [PubMed]
  • Kongsuwan K, Yu Q, Vincent A, Frisardi MC, Rosbash M, Lengyel JA, Merriam J. A Drosophila Minute gene encodes a ribosomal protein. Nature. 1985 Oct 10;317(6037):555–558. [PubMed]
  • Kongsuwan K, Dellavalle RP, Merriam JR. Deficiency Analysis of the Tip of Chromosome 3R in DROSOPHILA MELANOGASTER. Genetics. 1986 Mar;112(3):539–550. [PMC free article] [PubMed]
  • Korn ED. Biochemistry of actomyosin-dependent cell motility (a review). Proc Natl Acad Sci U S A. 1978 Feb;75(2):588–599. [PMC free article] [PubMed]
  • Lindsley DL, Sandler L, Baker BS, Carpenter AT, Denell RE, Hall JC, Jacobs PA, Miklos GL, Davis BK, Gethmann RC, et al. Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics. 1972 May;71(1):157–184. [PMC free article] [PubMed]
  • Mahaffey JW, Coutu MD, Fyrberg EA, Inwood W. The flightless Drosophila mutant raised has two distinct genetic lesions affecting accumulation of myofibrillar proteins in flight muscles. Cell. 1985 Jan;40(1):101–110. [PubMed]
  • Mogami K, Hotta Y. Isolation of Drosophila flightless mutants which affect myofibrillar proteins of indirect flight muscle. Mol Gen Genet. 1981;183(3):409–417. [PubMed]
  • Mogami K, O'Donnell PT, Bernstein SI, Wright TR, Emerson CP., Jr Mutations of the Drosophila myosin heavy-chain gene: effects on transcription, myosin accumulation, and muscle function. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1393–1397. [PMC free article] [PubMed]
  • Mullins JI, Casey JW, Nicolson MO, Burck KB, Davidson N. Sequence arrangement and biological activity of cloned feline leukemia virus proviruses from a virus-productive human cell line. J Virol. 1981 May;38(2):688–703. [PMC free article] [PubMed]
  • O'Donnell PT, Bernstein SI. Molecular and ultrastructural defects in a Drosophila myosin heavy chain mutant: differential effects on muscle function produced by similar thick filament abnormalities. J Cell Biol. 1988 Dec;107(6 Pt 2):2601–2612. [PMC free article] [PubMed]
  • Parker VP, Falkenthal S, Davidson N. Characterization of the myosin light-chain-2 gene of Drosophila melanogaster. Mol Cell Biol. 1985 Nov;5(11):3058–3068. [PMC free article] [PubMed]
  • Regan CL, Fuller MT. Interacting genes that affect microtubule function: the nc2 allele of the haywire locus fails to complement mutations in the testis-specific beta-tubulin gene of Drosophila. Genes Dev. 1988 Jan;2(1):82–92. [PubMed]
  • Taylor KA, Reedy MC, Córdova L, Reedy MK. Three-dimensional reconstruction of rigor insect flight muscle from tilted thin sections. Nature. 310(5975):285–291. [PubMed]
  • Toffenetti J, Mischke D, Pardue ML. Isolation and characterization of the gene for myosin light chain two of Drosophila melanogaster. J Cell Biol. 1987 Jan;104(1):19–28. [PMC free article] [PubMed]
  • Vibert P, Craig R. Structural changes that occur in scallop myosin filaments upon activation. J Cell Biol. 1985 Sep;101(3):830–837. [PMC free article] [PubMed]

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