• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Jul 1988; 119(3): 595–607.
PMCID: PMC1203445

Variation among Extracted Lines of Drosophila Melanogaster in Triacylglycerol and Carbohydrate Storage


Whole larvae and whole adult extracts from 26 second chromosome replacement lines of Drosophila melanogaster were analyzed to determine the amounts of stored triacylglycerols and carbohydrates as well as the activities of 13 enzymes in relevant biochemical pathways. Analysis of covariance revealed significant differences among lines in stored lipids and carbohydrates, as well as in activities of most of the enzymes. Significant broad-sense genetic correlations (among adjusted line means) were detected for a number of enzyme pairs. Multiple regression techniques were applied to assess the extent to which the amounts of stored triacylglycerols and carbohydrates could be predicted from the enzyme activities. Significant regressions were found in both adults and larvae, suggesting that modulation of enzyme activities is reflected in different sizes of storage pools. The population genetic consequences of natural selection acting on a phenotype such as energy storage is considered in light of models of metabolic flux in biochemical pathways.

Full Text

The Full Text of this article is available as a PDF (1.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barnes PT, Laurie-Ahlberg CC. Genetic variability of flight metabolism in Drosophila melanogaster. III. Effects of Gpdh allozymes and environmental temperature on power output. Genetics. 1986 Feb;112(2):267–294. [PMC free article] [PubMed]
  • Beenakkers AM. Carbohydrate and fat as a fuel for insect flight. A comparative study. J Insect Physiol. 1969 Mar;15(3):353–361. [PubMed]
  • Bijlsma R. Polymorphism at the G6pd and 6Pgd loci in Drosophila melanogaster. IV. Genetic factors modifying enzyme activity. Biochem Genet. 1980 Aug;18(7-8):699–715. [PubMed]
  • Bucolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem. 1973 May;19(5):476–482. [PubMed]
  • Burton RS, La Spada A. Trehalase polymorphism in Drosophila melanogaster. Biochem Genet. 1986 Oct;24(9-10):715–719. [PubMed]
  • Burton RS, Place AR. Evolution of selective neutrality: further considerations. Genetics. 1986 Nov;114(3):1033–1039. [PMC free article] [PubMed]
  • Cavener DR, Clegg MT. Evidence for biochemical and physiological differences between enzyme genotypes in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4444–4447. [PMC free article] [PubMed]
  • Clark AG. Natural selection with nuclear and cytoplasmic transmission. II. Tests with Drosophila from diverse populations. Genetics. 1985 Sep;111(1):97–112. [PMC free article] [PubMed]
  • Connors EM, Curtsinger JW. Relationship between alpha-glycerophosphate dehydrogenase activity and metabolic rate during flight in Drosophila melanogaster. Biochem Genet. 1986 Apr;24(3-4):245–257. [PubMed]
  • De Jong G, Scharloo W. Environmental determination of selective significance or neutrality of amylase variants in Drosophila melanogaster. Genetics. 1976 Sep;84(1):77–94. [PMC free article] [PubMed]
  • Dykhuizen DE, Dean AM, Hartl DL. Metabolic flux and fitness. Genetics. 1987 Jan;115(1):25–31. [PMC free article] [PubMed]
  • Eanes WF, Bingham B, Hey J, Houle D. Targeted selection experiments and enzyme polymorphism: negative evidence for octanoate selection at the G6PD locus in Drosophila melanogaster. Genetics. 1985 Feb;109(2):379–391. [PMC free article] [PubMed]
  • Geer BW, Laurie-Ahlberg CC. Genetic variation in the dietary sucrose modulation of enzyme activities in Drosophila melanogaster. Genet Res. 1984 Jun;43(3):307–321. [PubMed]
  • Geer BW, Bowman JT, Simmons JR. The pentose shunt in wild-type and glucose-6-phosphate dehydrogenase deficient Drosophila melanogaster. J Exp Zool. 1974 Jan;187(1):77–86. [PubMed]
  • Geer BW, Langevin ML, McKechnie SW. Dietary ethanol and lipid synthesis in Drosophila melanogaster. Biochem Genet. 1985 Aug;23(7-8):607–622. [PubMed]
  • Heinrich R, Rapoport SM. The utility of mathematical models for the understanding of metabolic systems. Biochem Soc Trans. 1983 Jan;11(1):31–35. [PubMed]
  • Kacser H, Burns JA. The control of flux. Symp Soc Exp Biol. 1973;27:65–104. [PubMed]
  • Kacser H, Burns JA. MOlecular democracy: who shares the controls? Biochem Soc Trans. 1979 Oct;7(5):1149–1160. [PubMed]
  • Kacser H, Burns JA. The molecular basis of dominance. Genetics. 1981 Mar-Apr;97(3-4):639–666. [PMC free article] [PubMed]
  • Lande R. The Genetic Covariance between Characters Maintained by Pleiotropic Mutations. Genetics. 1980 Jan;94(1):203–215. [PMC free article] [PubMed]
  • Laurie-Ahlberg CC, Maroni G, Bewley GC, Lucchesi JC, Weir BS. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. [PMC free article] [PubMed]
  • Laurie-Ahlberg CC, Wilton AN, Curtsinger JW, Emigh TH. Naturally occurring enzyme activity variation in Drosophila melanogaster. I. Sources of variation for 23 enzymes. Genetics. 1982 Oct;102(2):191–206. [PMC free article] [PubMed]
  • Laurie-Ahlberg CC, Barnes PT, Curtsinger JW, Emigh TH, Karlin B, Morris R, Norman RA, Wilton AN. Genetic variability of flight metabolism in Drosophila melanogaster. II. Relationship between power output and enzyme activity levels. Genetics. 1985 Dec;111(4):845–868. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Middleton RJ, Kacser H. Enzyme variation, metabolic flux and fitness: alcohol dehydrogenase in Drosophila melanogaster. Genetics. 1983 Nov;105(3):633–650. [PMC free article] [PubMed]
  • Miyashita N, Laurie-Ahlberg CC. Genetical analysis of chromosomal interaction effects on the activities of the glucose 6-phosphate and 6-phosphogluconate dehydrogenases in Drosophila melanogaster. Genetics. 1984 Apr;106(4):655–668. [PMC free article] [PubMed]
  • Miyashita N, Laurie-Ahlberg CC. Developmental variation in effects of the second and third chromosomes on the activities of the glucose 6-phosphate and 6-phosphogluconate dehydrogenases in Drosophila melanogaster. Biochem Genet. 1986 Jun;24(5-6):447–467. [PubMed]
  • Powers DA, DiMichele L, Place AR. The use of enzyme kinetics to predict differences in cellular metabolism, developmental rate, and swimming performance between LDH-B genotypes of the fish, fundulus heteroclitus. Isozymes Curr Top Biol Med Res. 1983;10:147–170. [PubMed]
  • RAABO E, TERKILDSEN TC. On the enzymatic determination of blood glucose. Scand J Clin Lab Invest. 1960;12(4):402–407. [PubMed]
  • Roe JH, Dailey RE. Determination of glycogen with the anthrone reagent. Anal Biochem. 1966 May;15(2):245–250. [PubMed]
  • Teague BD, Clark AG, Doane WW. Developmental analysis of lipids from wild-type and adipose60 mutants of Drosophila melanogaster. J Exp Zool. 1986 Oct;240(1):95–104. [PubMed]
  • Watt WB. Adaptation at Specific Loci. II. Demographic and Biochemical Elements in the Maintenance of the Colias Pgi Polymorphism. Genetics. 1983 Apr;103(4):691–724. [PMC free article] [PubMed]
  • Watt WB, Cassin RC, Swan MS. Adaptation at Specific Loci. III. Field Behavior and Survivorship Differences among Colias Pgi Genotypes Are Predictable from IN VITRO Biochemistry. Genetics. 1983 Apr;103(4):725–739. [PMC free article] [PubMed]
  • Watt WB, Carter PA, Blower SM. Adaptation at specific loci. IV. Differential mating success among glycolytic allozyme genotypes of Colias butterflies. Genetics. 1985 Jan;109(1):157–175. [PMC free article] [PubMed]
  • Westerhoff HV, Groen AK, Wanders RJ. Modern theories of metabolic control and their applications (review). Biosci Rep. 1984 Jan;4(1):1–22. [PubMed]
  • Wilton AN, Laurie-Ahlberg CC, Emigh TH, Curtsinger JW. Naturally occurring enzyme activity variation in Drosophila melanogaster. II. Relationships among enzymes. Genetics. 1982 Oct;102(2):207–221. [PMC free article] [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...