Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. 1988 Jul; 119(3): 549–559.
PMCID: PMC1203441

Allelic and Ectopic Recombination between Ty Elements in Yeast


Allelic and nonallelic (ectopic) recombination events were analyzed in a set of isogenic strains that carry marked Ty elements. We found that allelic recombination between Ty elements occurred at normal frequencies both in meiosis and mitosis. The marked Ty elements were involved in a large variety of different types of ectopic recombination and this variety was greater in mitosis than in meiosis. Allelic and ectopic recombination events occurred at similar frequencies in mitosis, but allelic recombination predominated in meiosis. Some of the types of ectopic mitotic recombination indicated the common occurrence of concerted recombination events. The length of homology represented by a delta element (330 bp) seemed to be sufficient for some types of mitotic and meiotic recombination.

Full Text

The Full Text of this article is available as a PDF (1.1M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Boeke JD, LaCroute F, Fink GR. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984;197(2):345–346. [PubMed]
  • Botstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene. 1979 Dec;8(1):17–24. [PubMed]
  • Breilmann D, Gafner J, Ciriacy M. Gene conversion and reciprocal exchange in a Ty-mediated translocation in yeast. Curr Genet. 1985;9(7):553–560. [PubMed]
  • Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol. 1983;100:293–308. [PubMed]
  • Chaleff DT, Fink GR. Genetic events associated with an insertion mutation in yeast. Cell. 1980 Aug;21(1):227–237. [PubMed]
  • Downs KM, Brennan G, Liebman SW. Deletions extending from a single Ty1 element in Saccharomyces cerevisiae. Mol Cell Biol. 1985 Dec;5(12):3451–3457. [PMC free article] [PubMed]
  • FOGEL S, HURST DD. Coincidence relations between gene conversion and mitotic recombination in Saccharomyces. Genetics. 1963 Mar;48:321–328. [PMC free article] [PubMed]
  • Jinks-Robertson S, Petes TD. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. [PMC free article] [PubMed]
  • Kingsman AJ, Gimlich RL, Clarke L, Chinault AC, Carbon J. Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae. J Mol Biol. 1981 Feb 5;145(4):619–632. [PubMed]
  • Klein HL, Petes TD. Genetic mapping of Ty elements in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Feb;4(2):329–339. [PMC free article] [PubMed]
  • Kohli J, Munz P, Aebi R, Amstutz H, Gysler C, Heyer WD, Lehmann L, Schuchert P, Szankasi P, Thuriaux P, et al. Interallelic and intergenic conversion in three serine tRNA genes of Schizosaccharomyces pombe. Cold Spring Harb Symp Quant Biol. 1984;49:31–40. [PubMed]
  • Lehrman MA, Goldstein JL, Russell DW, Brown MS. Duplication of seven exons in LDL receptor gene caused by Alu-Alu recombination in a subject with familial hypercholesterolemia. Cell. 1987 Mar 13;48(5):827–835. [PubMed]
  • Liebman S, Shalit P, Picologlou S. Ty elements are involved in the formation of deletions in DEL1 strains of Saccharomyces cerevisiae. Cell. 1981 Nov;26(3 Pt 1):401–409. [PubMed]
  • Liskay RM, Stachelek JL. Evidence for intrachromosomal gene conversion in cultured mouse cells. Cell. 1983 Nov;35(1):157–165. [PubMed]
  • Mikus MD, Petes TD. Recombination between genes located on nonhomologous chromosomes in Saccharomyces cerevisiae. Genetics. 1982 Jul-Aug;101(3-4):369–404. [PMC free article] [PubMed]
  • Nicholls RD, Fischel-Ghodsian N, Higgs DR. Recombination at the human alpha-globin gene cluster: sequence features and topological constraints. Cell. 1987 May 8;49(3):369–378. [PubMed]
  • Potier S, Winsor B, Lacroute F. Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae. Mol Cell Biol. 1982 Sep;2(9):1025–1032. [PMC free article] [PubMed]
  • Roeder GS, Fink GR. DNA rearrangements associated with a transposable element in yeast. Cell. 1980 Aug;21(1):239–249. [PubMed]
  • Roeder GS, Smith M, Lambie EJ. Intrachromosomal movement of genetically marked Saccharomyces cerevisiae transposons by gene conversion. Mol Cell Biol. 1984 Apr;4(4):703–711. [PMC free article] [PubMed]
  • Rothstein R. Deletions of a tyrosine tRNA gene in S. cerevisiae. Cell. 1979 May;17(1):185–190. [PubMed]
  • Rothstein R. Double-strand-break repair, gene conversion, and postdivision segregation. Cold Spring Harb Symp Quant Biol. 1984;49:629–637. [PubMed]
  • Rothstein R, Helms C, Rosenberg N. Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Mar;7(3):1198–1207. [PMC free article] [PubMed]
  • Rouyer F, Simmler MC, Page DC, Weissenbach J. A sex chromosome rearrangement in a human XX male caused by Alu-Alu recombination. Cell. 1987 Nov 6;51(3):417–425. [PubMed]
  • Scherer S, Davis RW. Recombination of dispersed repeated DNA sequences in yeast. Science. 1980 Sep 19;209(4463):1380–1384. [PubMed]
  • Warmington JR, Waring RB, Newlon CS, Indge KJ, Oliver SG. Nucleotide sequence characterization of Ty 1-17, a class II transposon from yeast. Nucleic Acids Res. 1985 Sep 25;13(18):6679–6693. [PMC free article] [PubMed]
  • Warmington JR, Anwar R, Newlon CS, Waring RB, Davies RW, Indge KJ, Oliver SG. A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III. Nucleic Acids Res. 1986 Apr 25;14(8):3475–3485. [PMC free article] [PubMed]
  • Williamson VM. Transposable elements in yeast. Int Rev Cytol. 1983;83:1–25. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    NCBI Bookshelf books that cite the current articles.
  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...