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Genetics. Mar 1988; 118(3): 461–470.
PMCID: PMC1203300

A Stable Genomic Source of P Element Transposase in Drosophila Melanogaster


A single P element insert in Drosophila melanogaster, called P[ry(+)Δ2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for ``helper'' plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bingham PM, Kidwell MG, Rubin GM. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. [PubMed]
  • Daniels SB, McCarron M, Love C, Chovnick A. Dysgenesis-induced instability of rosy locus transformation in Drosophila melanogaster: analysis of excision events and the selective recovery of control element deletions. Genetics. 1985 Jan;109(1):95–117. [PMC free article] [PubMed]
  • Daniels SB, McCarron M, Love C, Clark SH, Chovnick A. The underlying bases of gene expression differences in stable transformants of the rosy locus in Drosophila melanogaster. Genetics. 1986 Jun;113(2):265–285. [PMC free article] [PubMed]
  • Engels WR. Extrachromosomal control of mutability in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4011–4015. [PMC free article] [PubMed]
  • Engels WR. The estimation of mutation rates when premeiotic events are involved. Environ Mutagen. 1979;1(1):37–43. [PubMed]
  • Engels WR. The P family of transposable elements in Drosophila. Annu Rev Genet. 1983;17:315–344. [PubMed]
  • Engels WR. A trans-acting product needed for P factor transposition in Drosophila. Science. 1984 Dec 7;226(4679):1194–1196. [PubMed]
  • Engels WR, Benz WK, Preston CR, Graham PL, Phillis RW, Robertson HM. Somatic effects of P element activity in Drosophila melanogaster: pupal lethality. Genetics. 1987 Dec;117(4):745–757. [PMC free article] [PubMed]
  • Green MM. Genetic instability in Drosophila melanogaster: De novo induction of putative insertion mutations. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3490–3493. [PMC free article] [PubMed]
  • Hazelrigg T, Levis R, Rubin GM. Transformation of white locus DNA in drosophila: dosage compensation, zeste interaction, and position effects. Cell. 1984 Feb;36(2):469–481. [PubMed]
  • Karess RE, Rubin GM. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. [PubMed]
  • Laski FA, Rio DC, Rubin GM. Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing. Cell. 1986 Jan 17;44(1):7–19. [PubMed]
  • Laurie-Ahlberg CC, Stam LF. Use of P-element-mediated transformation to identify the molecular basis of naturally occurring variants affecting Adh expression in Drosophila melanogaster. Genetics. 1987 Jan;115(1):129–140. [PMC free article] [PubMed]
  • Levis R, Hazelrigg T, Rubin GM. Effects of genomic position on the expression of transduced copies of the white gene of Drosophila. Science. 1985 Aug 9;229(4713):558–561. [PubMed]
  • O'Hare K, Rubin GM. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. [PubMed]
  • Rio DC, Laski FA, Rubin GM. Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell. 1986 Jan 17;44(1):21–32. [PubMed]
  • Salz HK, Cline TW, Schedl P. Functional changes associated with structural alterations induced by mobilization of a P element inserted in the Sex-lethal gene of Drosophila. Genetics. 1987 Oct;117(2):221–231. [PMC free article] [PubMed]
  • Searles LL, Jokerst RS, Bingham PM, Voelker RA, Greenleaf AL. Molecular cloning of sequences from a Drosophila RNA polymerase II locus by P element transposon tagging. Cell. 1982 Dec;31(3 Pt 2):585–592. [PubMed]
  • Simmons MJ, Raymond JD, Johnson NA, Fahey TM. A comparison of mutation rates for specific loci and chromosome regions in dysgenic hybrid males of Drosophila melanogaster. Genetics. 1984 Jan;106(1):85–94. [PMC free article] [PubMed]
  • Spradling AC, Rubin GM. The effect of chromosomal position on the expression of the Drosophila xanthine dehydrogenase gene. Cell. 1983 Aug;34(1):47–57. [PubMed]

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