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Genetics. Dec 1987; 117(4): 633–643.
PMCID: PMC1203237

Intrachromosomal Recombination in Saccharomyces cerevisiae : Reciprocal Exchange in an Inverted Repeat and Associated Gene Conversion

Abstract

Intrachromosomal gene conversion has not shown a strong association with reciprocal exchanges. However, reciprocal exchanges do occur between intrachromosomal repeats. To understand the relationship between reciprocal exchange and gene conversion in repeated sequences the recombination behavior of an inverted repeat was studied. We have found that in one orientation a single copy of the kanr gene of the bacterial transposon Tn903 flanked by part of the inverted repeats IS903 does not give G418 resistance in Saccharomyces cerevisiae. A reciprocal exchange in the IS903 repeats inverts the kan r gene, which then gives G418 resistance in a single copy. Using this as a selection for intrachromosomal reciprocal exchange we have introduced multiple restriction site heterologies into the IS903 repeats and examined the crossover products for associated gene conversions. Approximately 50% of crossovers, both in mitosis and meiosis, were associated with a gene conversion. This suggests that these crossovers result from an intermediate that gives a gene conversion in 50% of the events, that is, both reciprocal exchange and gene conversion between repeated sequences have a common origin. The data are most consistent with a heteroduplex mismatch repair mechanism.

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Selected References

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  • Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. [PubMed]
  • Fogel S, Mortimer R, Lusnak K, Tavares F. Meiotic gene conversion: a signal of the basic recombination event in yeast. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1325–1341. [PubMed]
  • Game JC, Zamb TJ, Braun RJ, Resnick M, Roth RM. The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast. Genetics. 1980 Jan;94(1):51–68. [PMC free article] [PubMed]
  • Jackson JA, Fink GR. Gene conversion between duplicated genetic elements in yeast. Nature. 1981 Jul 23;292(5821):306–311. [PubMed]
  • Klar AJ, Strathern JN. Resolution of recombination intermediates generated during yeast mating type switching. Nature. 310(5980):744–748. [PubMed]
  • Liskay RM, Stachelek JL, Letsou A. Homologous recombination between repeated chromosomal sequences in mouse cells. Cold Spring Harb Symp Quant Biol. 1984;49:183–189. [PubMed]
  • Nomura N, Yamagishi H, Oka A. Isolation and characterization of transducing coliphage fd carrying a kanamycin resistance gene. Gene. 1978 Feb;3(1):39–51. [PubMed]
  • Hurst DD, Fogel S, Mortimer RK. Conversion-associated recombination in yeast (hybrids-meiosis-tetrads-marker loci-models). Proc Natl Acad Sci U S A. 1972 Jan;69(1):101–105. [PMC free article] [PubMed]
  • Kunkel TA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. [PMC free article] [PubMed]
  • Nicolas A, Rossignol JL. Gene conversion: point-mutation heterozygosities lower heteroduplex formation. EMBO J. 1983;2(12):2265–2270. [PMC free article] [PubMed]
  • Perozzi G, Prakash S. RAD7 gene of Saccharomyces cerevisiae: transcripts, nucleotide sequence analysis, and functional relationship between the RAD7 and RAD23 gene products. Mol Cell Biol. 1986 May;6(5):1497–1507. [PMC free article] [PubMed]
  • Rossignol JL, Nicolas A, Hamza H, Langin T. Origins of gene conversion and reciprocal exchange in Ascobolus. Cold Spring Harb Symp Quant Biol. 1984;49:13–21. [PubMed]
  • Russell DW, Jensen R, Zoller MJ, Burke J, Errede B, Smith M, Herskowitz I. Structure of the Saccharomyces cerevisiae HO gene and analysis of its upstream regulatory region. Mol Cell Biol. 1986 Dec;6(12):4281–4294. [PMC free article] [PubMed]
  • Roman H, Fabre F. Gene conversion and associated reciprocal recombination are separable events in vegetative cells of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6912–6916. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Shen P, Huang HV. Homologous recombination in Escherichia coli: dependence on substrate length and homology. Genetics. 1986 Mar;112(3):441–457. [PMC free article] [PubMed]
  • Stadler DR. The mechanism of intragenic recombination. Annu Rev Genet. 1973;7:113–127. [PubMed]
  • Struhl K, Davis RW. Position effects in Saccharomyces cerevisiae. J Mol Biol. 1981 Nov 5;152(3):569–575. [PubMed]
  • Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW. The double-strand-break repair model for recombination. Cell. 1983 May;33(1):25–35. [PubMed]
  • Wagstaff JE, Klapholz S, Waddell CS, Jensen L, Esposito RE. Meiotic exchange within and between chromosomes requires a common Rec function in Saccharomyces cerevisiae. Mol Cell Biol. 1985 Dec;5(12):3532–3544. [PMC free article] [PubMed]

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