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Genetics. 1985 Jun; 110(2): 173–185.
PMCID: PMC1202558

Physiological Characterization of Adaptive Clones in Evolving Populations of the Yeast, SACCHAROMYCES CEREVISIAE


Populations of a diploid strain of S. cerevisiae were grown in glucose-limited continuous culture for more than 260 generations. A series of seven sequential adaptive changes were identified by monitoring the frequency of cycloheximide resistance in these populations. Samples were taken from the continuous cultures following each adaptive shift and characterized physiologically to determine (1) the range of phenotypes that can be selected in a precisely defined constant environment and (2) the order and predictability of the occurrence of the adaptive mutations in evolving populations. The clones were characterized with respect to the growth parameters, maximum growth rate, saturation coefficient and yield, as well as for changes in cell size and geometry and rate of glucose uptake. The maximum growth rates of the seven adaptive clones were very similar, but in contrast the saturation coefficients differed substantially. Surprisingly, not all clones showed reductions in the saturation coefficients, in comparison to the immediately preceding clones, as would be predicted from classical continuous culture kinetics. In addition, yield estimates first increased and then decreased for later isolated adaptive clones. In general, the results suggest epistatic interactions between the adaptive clones, consistent with earlier published results. The rate of glucose uptake, as measured by 14Cxylose uptake, increased dramatically after the selection and fixation of seven adaptive clones. Progressive decreases in cell volume and changes in cell geometry, resulting in increased surface area to volume ratios, were also observed in the adaptive clones, but these changes were not always seen in other haploid and diploid yeast populations evolving under the same conditions. Such changes may be easily explainable in terms of the characteristics of the glucose-limited environment. The significance of the results to the evolution of microorganisms under nutrient-limiting conditions is discussed.

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Selected References

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  • Beck C, von Meyenburg HK. Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation. J Bacteriol. 1968 Aug;96(2):479–486. [PMC free article] [PubMed]
  • CIRILLO VP. Mechanism of glucose transport across the yeast cell membrane. J Bacteriol. 1962 Sep;84:485–491. [PMC free article] [PubMed]
  • Hennaut C, Hilger F, Grenson M. Space limitation for permease insertion in the cytoplasmic membrane of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1970 May 22;39(4):666–671. [PubMed]
  • Neijssel OM, Tempest DW. Bioenergetic aspects of aerobic growth of Klebsiella aerogenes NCTC 418 in carbon-limited and carbon-sufficient chemostat culture. Arch Microbiol. 1976 Mar 19;107(2):215–221. [PubMed]
  • POWELL EO. Criteria for the growth of contaminants and mutants in continuous culture. J Gen Microbiol. 1958 Feb;18(1):259–268. [PubMed]
  • RAABO E, TERKILDSEN TC. On the enzymatic determination of blood glucose. Scand J Clin Lab Invest. 1960;12(4):402–407. [PubMed]
  • van Uden N. Transport-limited fermentation and growth of saccharomyces cerevisiae and its competitive inhibition. Arch Mikrobiol. 1967;58(2):155–168. [PubMed]

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