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Genetics. Aug 1984; 107(4): 611–634.
PMCID: PMC1202380

Organization and Mapping of a Sequence on the DROSOPHILA MELANOGASTER X and Y Chromosomes That Is Transcribed during Spermatogenesis


The D. melanogaster DNA segment in the recombinant phage λDm2L1 contains at least eight copies of a tandemly repeated 1250-base pair (bp) sequence (henceforth called the 2L1 sequence). Testes from XO D. melanogaster males contain an abundant 800-base RNA species that is homologous to a 520-bp region of the 2L1 sequence. Blotting experiments show that the 2L1 sequence is repeated in the D. melanogaster genome and is present on both the X and Y chromosomes. With the use of X-Y translocations, the 2L1 sequence has been mapped to a region between kl-1 and kl-2 on the long arm of the Y chromosome. In Oregon-R wild type there are an estimated 200 copies of the 2L1 sequence on the X chromosome and probably at least 80 copies on the Y chromosome. In some other strains the repetition frequency on the Y chromosome is about the same, but the copy number on the X chromosome is much reduced. On the basis of the five strains investigated, there is a correlation between copy number of the 2L1 sequence on the X chromosome and the presence of a particular allele of the Stellate locus (Ste; 1-45.7). It seems that low copy number corresponds to Ste + and high copy number corresponds to Ste. The Ste locus determines whether single or star-shaped crystals are observed in the spermatocytes of XO males. Studies using D. simulans and D. mauritiana DNA show that the 2L1 sequence is homologous to restriction fragments in male DNA but not female DNA, indicating that this sequence is present only on the Y chromosome in these two species. In DNA derived from D. erecta, D. teissieri and D. yakuba, there is very little, if any, hybridization with the 2L1 sequence probe.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ashburner M, Bonner JJ. The induction of gene activity in drosophilia by heat shock. Cell. 1979 Jun;17(2):241–254. [PubMed]
  • Bingham PM, Levis R, Rubin GM. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. [PubMed]
  • Leigh Brown AJ, Ish-Horowicz D. Evolution of the 87A and 87C heat-shock loci in Drosophila. Nature. 1981 Apr 23;290(5808):677–682. [PubMed]
  • Gausz J, Bencze G, Gyurkovics H, Ashburner M, Ish-Horowicz D, Holden JJ. Genetic Characterization of the 87c Region of the Third Chromosome of DROSOPHILA MELANOGASTER. Genetics. 1979 Dec;93(4):917–934. [PMC free article] [PubMed]
  • Hardy RW, Lindsley DL, Livak KJ, Lewis B, Siversten AL, Joslyn GL, Edwards J, Bonaccorsi S. Cytogenetic analysis of a segment of the Y chromosome of Drosophila melanogaster. Genetics. 1984 Aug;107(4):591–610. [PMC free article] [PubMed]
  • MEYER GF, HESS O, BEERMANN W. [Phase specific function structure in spermatocyte nuclei of Drosophila melanogaster and their dependence of Y chromosomes]. Chromosoma. 1961;12:676–716. [PubMed]
  • Orgel LE, Crick FH. Selfish DNA: the ultimate parasite. Nature. 1980 Apr 17;284(5757):604–607. [PubMed]
  • Pierce DA, Lucchesi JC. Analysis of a dispersed repetitive DNA sequence in isogenic lines of Drosophila. Chromosoma. 1981;82(4):471–492. [PubMed]
  • Spradling AC, Rubin GM. Drosophila genome organization: conserved and dynamic aspects. Annu Rev Genet. 1981;15:219–264. [PubMed]
  • Tchurikov NA, Ilyin YV, Skryabin KG, Ananiev EV, Bayev AA, Jr, Krayev AS, Zelentsova ES, Kulguskin VV, Lyubomirskaya NV, Georgiev GP. General properties of mobile dispersed genetic elements in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):655–665. [PubMed]
  • Livak KJ, Freund R, Schweber M, Wensink PC, Meselson M. Sequence organization and transcription at two heat shock loci in Drosophila. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5613–5617. [PMC free article] [PubMed]
  • Young MW, Schwartz HE. Nomadic gene families in Drosophila. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):629–640. [PubMed]

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