• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Jun 1983; 104(2): 257–270.
PMCID: PMC1202075

Nullisomic Tetrahymena. II. a Set of Nullisomics Define the Germinal Chromosomes

Abstract

Crosses of a diploid Tetrahymena thermophila to a strain with a haploid germinal nucleus result in chromosome loss during meiosis in the haploid. The resulting monosomics can be made nullisomic by a special cross that induces homozygosis of a meiotic product of the germinal nucleus, but retention of the parental somatic nucleus. The creation and testing of single nullisomics for three of the five chromosome pairs and a triple nullisomic missing another pair is presented. Taken together, these strains make possible a series of crosses in which all but one of the chromosomes is missing in one parent. This set of nullisomics can, therefore, be used to map any mutation in Tetrahymena to a specific chromosome.

Full Text

The Full Text of this article is available as a PDF (1.4M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Frankel J, Jenkins LM, Doerder FP, Nelsen EM. Mutations affecting cell division in Tetrahymena pyriformis. I. Selection and genetic analysis. Genetics. 1976 Jul;83(3 PT2):489–506. [PMC free article] [PubMed]
  • Gorovsky MA. Genome organization and reorganization in Tetrahymena. Annu Rev Genet. 1980;14:203–239. [PubMed]
  • Martindale DW, Allis CD, Bruns PJ. Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages. Exp Cell Res. 1982 Jul;140(1):227–236. [PubMed]
  • Mayo KA, Orias E. Further evidence for lack of gene expression in the Tetrahymena micronucleus. Genetics. 1981 Aug;98(4):747–762. [PMC free article] [PubMed]
  • Nanney DL, McCoy JW. Characterization of the species of the Tetrahymena pyriformis complex. Trans Am Microsc Soc. 1976 Oct;95(4):664–682. [PubMed]
  • Orias E, Flacks M. Macronuclear genetics of Tetrahymena. I. Random distribution of macronuclear genecopies in T. pyriformis, syngen 1. Genetics. 1975 Feb;79(2):187–206. [PMC free article] [PubMed]
  • Wellnitz WR, Bruns PJ. The pre-pairing events in Tetrahymena thermophila. Analysis of blocks imposed by high concentrations of Tris-Cl. Exp Cell Res. 1979 Mar 1;119(1):175–180. [PubMed]
  • Yao MC, Gorovsky MA. Comparison of the sequences of macro- and micronuclear DNA of Tetrahymena pyriformis. Chromosoma. 1974;48(1):1–18. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...