Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Dec 1982; 102(4): 725–736.
PMCID: PMC1201969

Essential Genes and Deficiencies in the UNC-22 IV Region of CAENORHABDITIS ELEGANS

Abstract

Five formaldehyde-induced deficiencies that uncover unc-22 IV, a gene affecting muscle structure in the nematode Caenorhabditis elegans were isolated and positioned. The largest deficiency, sDf2, extends in both directions from unc-22 and is approximately 1.0–2.0 map units in length. The other four deficiencies, sDf7, sDf8, sDf9 and sDf10, are all smaller than sDf2 and are located within the region uncovered by this deficiency. Thirty-seven ethyl methanesulfonate-induced lethal and sterile mutations linked to unc-22 were isolated and tested for complementation with sDf2. Nineteen lethal mutations failed to complement sDf2. Sixteen of these were further positioned by recombination mapping and also by deficiency mapping with sDf7, sDf8, sDf9 and sDf10. These sixteen mutations define 11 new essential genes in this region. Eight of the genes lie in a 0.9-map unit interval to the left of unc-22, whereas the three remaining genes lie in a region of about 0.2 map units to the right of unc-22. We believe that two of the essential genes identified in this study, let-56 and let-52, are the adjacent genes on either side of unc-22. The lethal mutations exhibit a wide range of terminal phenotypes: from first stage larva to sterile adult.

Full Text

The Full Text of this article is available as a PDF (703K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Hilliker AJ, Clark SH, Chovnick A, Gelbart WM. Cytogenetic analysis of the chromosomal region immediately adjacent to the rosy locus in Drosophila melanogaster. Genetics. 1980 May;95(1):95–110. [PMC free article] [PubMed]
  • Hochman B. Analysis of chromosome 4 in Drosophila melanogaster. II. Ethyl methanesulfonate induced lethals. Genetics. 1971 Feb;67(2):235–252. [PMC free article] [PubMed]
  • Horvitz HR, Brenner S, Hodgkin J, Herman RK. A uniform genetic nomenclature for the nematode Caenorhabditis elegans. Mol Gen Genet. 1979 Sep;175(2):129–133. [PubMed]
  • Meneely PM, Herman RK. Lethals, steriles and deficiencies in a region of the X chromosome of Caenorhabditis elegans. Genetics. 1979 May;92(1):99–115. [PMC free article] [PubMed]
  • Meneely PM, Herman RK. Suppression and function of X-linked lethal and sterile mutations in Caenorhabditis elegans. Genetics. 1981 Jan;97(1):65–84. [PMC free article] [PubMed]
  • Moerman DG, Baillie DL. Formaldehyde mutagenesis in the nematode Caenorhabditis elegans. Mutat Res. 1981 Feb;80(2):273–279. [PubMed]
  • Waterston RH, Thomson JN, Brenner S. Mutants with altered muscle structure of Caenorhabditis elegans. Dev Biol. 1980 Jun 15;77(2):271–302. [PubMed]
  • Woodruff RC, Ashburner M. The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for alcohol dehydrogenase. II. Lethal mutations in the region. Genetics. 1979 May;92(1):133–149. [PMC free article] [PubMed]
  • Zengel JM, Epstein HF. Identification of genetic elements associated with muscle structure in the nematode Caenorhabditis elegans. Cell Motil. 1980;1(1):73–97. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...