The sweetness-inducing effect of miraculin; behavioural and neurophysiological experiments in the rhesus monkey Macaca mulatta

J Physiol. 1983 April; 337: 221–240.

PMCID: PMC1199104

The sweetness-inducing effect of miraculin; behavioural and neurophysiological experiments in the rhesus monkey Macaca mulatta

J. N. Brouwer,^* D. Glaser,^† C. Hard af Segerstad, G. Hellekant, Y. Ninomiya,^§ and H. van der Wel^*

Department of Veterinary Science, University of Wisconsin, Madison, WI 53706 U.S.A.

Wisconsin Regional Primate Center, Madison, WI 53706 U.S.A.

Unilever Research Laboratory, Vlaardingen, The Netherlands

Anthropologisches Institut der Universitat Zurich, Switzerland

^§On leave from the Department of Oral Physiology, Gifu College of Dentistry, Gifu, Japan.

This article has been cited by other articles in PMC.

Abstract

1. The gustatory effects of miraculin, the sweetness-inducing protein from the miracle fruit Synsepalum dulcificum, was studied in the rhesus monkey, Macaca mulatta.

2. The intake of five acids was recorded in two-bottle preference tests, one bottle containing acid and the other tap water, before and after miraculin treatment. All the acids tasted more pleasant after miraculin.

3. The electrical activity of the chorda tympani nerve to stimulation of the tongue with a variety of sweeteners, acids, sodium chloride and quinine hydrochloride was recorded in anaesthetized animals.

4. Pre-treatment of the tongue with 0·3-5 mg miraculin doubled the summated nerve response to the acids and diminished the response to sucrose by about 10%. The enhancement lasted for at least an hour and the diminution up to 20 min.

5. After miraculin treatment the Spearman's rank correlation coefficient between the order of increased intake of acids and the order of enhancement of the summated nerve response was 0·99.

6. A solution of 0·1 mg miraculin per ml. elicited a weak nerve response. No preference over water for this concentration of miraculin was recorded in the two-bottle tests.

7. The activity of twenty-nine single taste fibres, selected for their responsiveness to sweetness or acids or both, was recorded after miraculin treatment. Effects were obtained in nine fibres which were similar but more pronounced than those observed in the summated recordings. Before miraculin, these fibres responded better and to a larger variety of sweeteners (81%) than the other fibres (40%). After miraculin, acids elicited on the average 2·3 times more activity than before, while the response to sweeteners was depressed. In twenty fibres no effect of miraculin was observed. These fibres responded to fewer of the sweeteners and were more stimulated by the non-sweet stimuli than the first group.

8. The results suggest that miraculin acts on those structures in the taste cell membrane that are involved in perception of the sweet taste, making them sensitive to acids. The new quality of sweetness after miraculin treatment is signalled by taste fibres which normally respond to sweet substances but which, under the influence of miraculin, are responding to acids. It is likely that the quality of a taste stimulus is conveyed by the identity of the taste fibres.

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Selected References

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Bartoshuk LM, Gentile RL, Molkowitz HR, Meiselman HL. Sweet taste induced by miracle fruit (Synsepalum dulcificum). Physiol Behav. 1974 Mar;12(3):449–456. [PubMed]
Brouwer JN, van der Wel H, Francke A, Henning GJ. Mieraculin, the sweetness-inducing protein from miracle fruit. Nature. 1968 Oct 26;220(5165):373–374. [PubMed]
FISHMAN IY. Single fiber gustatory impulses in rat and hamster. J Cell Physiol. 1957 Apr;49(2):319–334. [PubMed]
Hellekant G. Electrophysiological investigation of the gustatory effect of ethyl alcohol. II. A single fibre analysis in the cat. Acta Physiol Scand. 1965 Aug;64(4):398–406. [PubMed]
Hellekant G. Inhibitory processes in gustation. Acta Physiol Scand. 1969 Jan–Feb;75(1):39–48. [PubMed]
Hellekant G. Reference for sweet taste and the taste of sweeteners in animals. Fortschr Tierphysiol Tierernahr. 1980;(11):43–52. [PubMed]
Hellekant G, Aronsson T, Karlbom U. Neural (electro-physiological) methods in chemoreception research. Fortschr Tierphysiol Tierernahr. 1980;(11):21–26. [PubMed]
Hellekant G, Glaser D, Brouwer JN, van der Wel H. Gustatory effects of miraculin, monellin and thaumatin in the Saguinus midas tamarin monkey studied with electrophysiological and behavioural techniques. Acta Physiol Scand. 1976 Jun;97(2):241–250. [PubMed]
Nagai T, Ueda K. Stochastic properties of gustatory impulse discharges in rat chorda tympani fibers. J Neurophysiol. 1981 Mar;45(3):574–592. [PubMed]
Ogawa H, Sato M, Yamashita S. Variability in impulse discharges in rat chorda tympani fibers in response to repeated gustatory stimulations. Physiol Behav. 1973 Oct;11(4):469–479. [PubMed]
Ogawa H, Yamashita S, Sato M. Variation in gustatory nerve fiber discharge pattern with change in stimulus concentration and quality. J Neurophysiol. 1974 May;37(3):443–457. [PubMed]
Perkel DH, Gerstein GL, Moore GP. Neuronal spike trains and stochastic point processes. I. The single spike train. Biophys J. 1967 Jul;7(4):391–418. [PubMed]
Pietra P, Miraldi A, Taglietti V. Pulse coding in the gustative afferent fibres of the frog gustatory system. Pflugers Arch. 1972;331(4):307–314. [PubMed]
PFAFFMANN C. Gustatory nerve impulses in rat, cat and rabbit. J Neurophysiol. 1955 Sep;18(5):429–440. [PubMed]
Zotterman Y. The neural mechanism of taste. Prog Brain Res. 1967;23:139–154. [PubMed]

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