• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Nov 1969; 115(2): 231–240.
PMCID: PMC1185094

Yeast cell-wall synthesis

Abstract

1. A study of wall synthesis has been made by following the incorporation of radioactive glucose and threonine into the cytoplasm and wall of yeast. 2. Both glucose and threonine are incorporated into a mannan glycopeptide. The glucose is also synthesized into a structural glucan of the wall. 3. The mannan glycopeptide contains high-molecular-weight mannan and low-molecular-weight mannose and oligosaccharide units composed of mannose. Both types of carbohydrate are attached to the peptide. The extent of radioactive incorporation into these different carbohydrate constituents of the glycopeptide remained constant during a pulse-chase experiment. No evidence of a sequential synthesis of oligosaccharides and high-molecular-weight mannan was obtained. 4. Cycloheximide inhibits the incorporation of threonine into the wall but only partially inhibits the incorporation of glucose. Thus not all the polysaccharide deposited into the wall is dependent on a simultaneous peptide synthesis and incorporation. 5. Protoplasts grown in an iso-osmotic medium secreted a mannan polymer that was probably a glycopeptide.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ALGRANATI ID, CARMINATTI H, CABIB E. THE ENZYMIC SYNTHESIS OF YEAST MANNAN. Biochem Biophys Res Commun. 1963 Aug 20;12:504–509. [PubMed]
  • Behrens NH, Cabib E. The biosynthesis of mannan in Saccharomyces carlsbergensis. J Biol Chem. 1968 Feb 10;243(3):502–509. [PubMed]
  • CHUNG CW, NICKERSON WJ. Polysaccharide syntheses in growing yeasts. J Biol Chem. 1954 May;208(1):395–407. [PubMed]
  • DOUNCE AL, WITTER RF, MONTY KJ, PATE S, COTTONE MA. A method for isolating intact mitochondria and nuclei from the same homogenate, and the influence of mitochondrial destruction on the properties of cell nuclei. J Biophys Biochem Cytol. 1955 Mar;1(2):139–153. [PMC free article] [PubMed]
  • EDDY AA. [The structure of the yeast cell wall. II. Degradative studies with enzymes]. Proc R Soc Lond B Biol Sci. 1958 Dec 17;149(936):425–440. [PubMed]
  • FALCONE G, NICKERSON WJ. Cell-wall mannan-protein of baker's yeast. Science. 1956 Aug 10;124(3215):272–273. [PubMed]
  • FRIIS J, OTTOLENGHI P. Localization of melibiase in a strain of veast. C R Trav Lab Carlsberg. 1959;31:272–281. [PubMed]
  • LOUTIT JS, PEARCE LE. MATING IN PSEUDOMONAS AERUGINOSA. Nature. 1965 Feb 20;205:822–822. [PubMed]
  • GILVARG C. Utilization of glucose-1-C14 by yeast. J Biol Chem. 1952 Nov;199(1):57–64. [PubMed]
  • Gits JJ, Grenson M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. 3. Evidence for a specific methionine-transporting system. Biochim Biophys Acta. 1967 Jul 3;135(3):507–516. [PubMed]
  • Grenson M, Mousset M, Wiame JM, Bechet J. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system. Biochim Biophys Acta. 1966 Oct 31;127(2):325–338. [PubMed]
  • KESSLER G, NICKERSON WJ. Glucomannan-protein complexes from cell walls of yeasts. J Biol Chem. 1959 Sep;234:2281–2285. [PubMed]
  • KORN ED, NORTHCOTE DH. Physical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall. Biochem J. 1960 Apr;75:12–17. [PMC free article] [PubMed]
  • Lampen JO. External enzymes of yeast: their nature and formation. Antonie Van Leeuwenhoek. 1968;34(1):1–18. [PubMed]
  • MCLELLAN WL, Jr, LAMPEN JO. The acid phosphatase of yeast. Localization and secretion by protoplasts. Biochim Biophys Acta. 1963 Feb 12;67:324–326. [PubMed]
  • MAW GA. The uptake of some sulphur-containing amino acids by a brewer's yeast. J Gen Microbiol. 1963 May;31:247–259. [PubMed]
  • MYERS FL, NORTHCOTE DH. Partial purification and some properties of a cellulase from Helix pomatia. Biochem J. 1959 Apr;71(4):749–756. [PMC free article] [PubMed]
  • NORTHCOTE DH, HORNE RW. The chemical composition and structure of the yeast cell wall. Biochem J. 1952 May;51(2):232–236. [PMC free article] [PubMed]
  • Sentandreu R, Northcote DH. The structure of a glycopeptide isolated from the yeast cell wall. Biochem J. 1968 Sep;109(3):419–432. [PMC free article] [PubMed]
  • Stoddart RW, Northcote DH. Metabolic relationships of the isolated fractions of the pectic substances of actively growing sycamore cells. Biochem J. 1967 Oct;105(1):45–59. [PMC free article] [PubMed]
  • TREVELYAN WE, GAMMON JN, WIGGINS EH, HARRISON JS. Studies on yeast metabolism. II. Synthesis of cell carbohydrates during glucose fermentation and its inhibition by azide. Biochem J. 1952 Jan;50(3):303–310. [PMC free article] [PubMed]
  • Vazquez D, Monro RE. Effects of some inhibitors of protein synthesis on the binding of aminoacyl tRNA to ribosomal subunits. Biochim Biophys Acta. 1967 Jun 20;142(1):155–173. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...