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Am J Hum Genet. Jun 2003; 72(6): 1586–1590.
PMCID: PMC1180321

South Asia, the Andamanese, and the Genetic Evidence for an “Early” Human Dispersal out of Africa

To the Editor:

The out-of-Africa model of anatomically modern human evolution posits an African origin 100,000–200,000 years ago, followed by subsequent dispersal(s) to Eurasia and other continents within the last 100,000 years (Stringer and Andrews 1988). Although alternative models have been proposed, the out-of-Africa scenario receives the most support both from archeological and genetic evidence (Lahr and Foley 1994). However, the route(s) followed by the African migrants remain poorly understood. One proposed route was through northern Africa toward the Levant, which finds support in the archeological and fossil records (Lahr and Foley 1994). This exit of modern humans out of Africa would have taken place during the Upper Paleolithic era (~45,000 years ago), which considerably postdates the earliest evidence of modern human presence in the Sahul. Indeed, luminescence dating, paleovegetation changes, and skeletal remains suggest that Australia was inhabited by modern humans by 60,000 years ago (Roberts and Jones 1994; Johnson et al. 1999; Miller et al. 1999; Thorne et al. 1999), implying a substantially earlier migration from Africa to Australia. To take this evidence into account, as well as morphological and archeological features of many Australian fossils, a second migration of modern humans, known as the “southern route” hypothesis, was suggested to have occurred during Middle Paleolithic times (60,000–100,000 years ago) from eastern Africa to Sahul via South Asia (Cavalli-Sforza et al. 1994; Lahr and Foley 1994).

In the January 2003 issue of the Journal, Endicott et al. (2003) investigated the genetic affinities of 11 Andaman islanders, a group of people in the Indian Ocean with phenotypic similarities to some African populations (i.e., “Negrito” features) and reputed to be possible descendants of early migrants out of Africa to Sahul, following the southern route. The authors claim that the results of their investigation “support the growing evidence of an early movement of humans through southern Asia.” In our opinion, Endicott and colleagues’ results do not support any relationship between the present Andamanese population and the hypothesized early southern migration. The authors identified three different mtDNA haplotypes in 11 Andaman islanders, two belonging to haplogroup M2 and one belonging to M4. These haplogroups had previously been reported only in the Indian subcontinent (Kivisild et al. 1999b; Bamshad et al. 2001). The Andaman M4 haplotype has been found previously in mainland India (Kivisild et al. 1999b), whereas the two Andaman M2 haplotypes are (so far) unique to the Andamanese. Given that (1) the latter two types occupy a basal position in the M2 network, which has an estimated coalescence time of 63,000±6,000 years (Kivisild et al. 1999b), and (2) they are not found in mainland India, Endicott et al. (2003) conclude they represent an “early” settlement of the Andaman Islands. These two points need discussion.

Regarding point 1, the age of a haplogroup cannot be automatically equated to the age of subsets of this haplogroup. The founding type of haplogroup M2, characterized by 16223T and 16319A relative to the Cambridge reference sequence (CRS) (Anderson et al. 1981) (fig. 1), is dated to 63,000 years but is still present in mainland India. This does not mean that any cluster branching off of this node is 63,000 years old, but rather that it is, at most, 63,000 years old. In principle, the Andaman M2 cluster could be dated to any time between 63,000 years and today.

Figure  1
Median joining network (after Endicott et al. 2003) of mtDNA haplogroup M2. Substitution positions relative to the CRS are shown. The number of assigned individuals from sampling to date is indicated in the relevant nodes.

Regarding point 2, we do not question the fact that the Andaman M2 haplotypes have not been found in mainland India. However, related sequences are found in mainland India, one of which is cited by Endicott et al. (2003) as a candidate ancestral haplotype of the Andaman M2 sequences, since it differs by only one substitution at 16274 from one Andaman M2 haplotype. From figure 2 of Endicott et al. (2003), we identify in mainland India another candidate ancestral haplotype for the Andaman M2 types, namely, the founder type of the entire M2 haplogroup (characterized by 16223T, 16319A). This haplotype differs from one Andaman haplotype by a single substitution (at 16357) and has already been reported in mainland India (Kivisild et al. 1999b). Therefore, although identical haplotypes are not found elsewhere, the Andaman M2 haplotypes have closely related counterparts in present-day mainland India.

The extent of isolation experienced by Andaman islanders (and, thus, an approximate upper time limit for the colonization of the archipelago) can be estimated by dating the time needed to accumulate the observed variation of the Andaman-specific mtDNA lineages. In this respect, we analyzed 42 unambiguously determined mtDNA sequences from Onge, Jarawa, and Great Andamanese individuals (Thangaraj et al. 2003) and six sequences from Aka-Bea individuals (Endicott et al. 2003) belonging to haplogroup M2 (on the basis of the motif 16223T/16319A). Altogether, the 48 Andaman M2 sequences defined five haplotypes unified by 16357C (fig. 1). First, we investigated the pattern of demographic history for the data set of 48 Andaman M2 sequences, by calculating Fu’s Fs (Fu 1997) and Tajima’s D (Tajima 1989) (P values are based on 1,000 simulated samples), as well as Harpending’s r (Harpending et al. 1993), as implemented in ARLEQUIN 2.0. Both Fu’s Fs and Tajima’s D were not significantly different from zero (Fs=-0.66, P=.35; D=-0.19, P=.50), suggesting no demographic expansion of the Andamanese population. In addition, Harpending’s r value was 0.146, consistent with a scenario of constant population size.

Assuming a constant population size, the Kimura two-parameter model of substitution (as implemented in MEGA 2.1) and a mutation rate of 1.65×10-7 per site per year (corresponding to 33% divergence per million years [Ward et al. 1991]), divergence times of the Andaman M2 haplotypes relative to two different founders were estimated. When the most ancestral Andaman-specific M2 type (bearing the motif 16223T, 16319A, 16357C) (fig. 1) was used as a founder, the average time needed to accumulate the observed variation in Andaman Islands was 25,300 years. When the Indian candidate as ancestral type of the Andaman M2 sequences (bearing the motif 16223T, 16319A) (fig. 1) was used as a founder, the average time back to the Indian common ancestor was 42,300 years. These results indicate that Andaman islanders may have remained isolated from mainland India for 25,000–42,000 years. However, this time is almost certainly overestimated. The average substitution rate of the five substitutions found in the Andaman M2 cluster (16213, 16234, 16311, 16344, 16357) (fig. 1) is ~2.5 times that of the control region as a whole (Meyer et al. 1999); thus, the real time to accumulate the observed variation may be up to 2.5-fold less, or 10,100–16,900 years. Moreover, the time estimates are based on the assumption that all the Andaman M2 types are derived from a single founding lineage; more recent times would result if there was more than one founding lineage. In summary, the presence of the unique M2 lineages in the Andaman Islands (as well as the Andaman M4 lineage that is identical to present-day Indian M4 lineages) suggests a colonization event from the Indian subcontinent occurring during the Upper Paleolithic (or perhaps even more recently), at most 40,000 years ago, which is still at least 20,000 years after the early southern-route migration to Sahul.

Thangaraj et al. (2003) also presented Y-chromosome evidence for Andaman islanders. They suggested that haplogroup D, an Asian-specific YAP lineage bearing the derived state at M174 (Underhill et al. 2000), is an original paternal lineage of Andaman islanders. To estimate the age of M174, we calculated that the average number of mutations from M174 to contemporary haplotypes was 4.89, on the basis of the data in Underhill et al. (2000). Underhill et al. (2000) estimated that the average time for a new mutation was 5,600–6,900 years, which puts the age of M174 at 27,000–34,000 years. Since the age of M174 is an upper estimate for the time when this Y haplogroup reached the Andaman Islands, the Y-chromosome evidence suggests an upper figure of ~30,000 years for the colonization of Andaman Islands, in good agreement with the mtDNA evidence. Hence, both Y-chromosome and mtDNA data support an Upper Paleolithic rather than Middle Paleolithic settlement of the archipelago.

Other studies have shown that mtDNA haplogroups M and U are common in the Indian subcontinent, and both have coalescence times of 50,000–70,000 years (Quintana-Murci et al. 1999; Kivisild et al. 1999a, 1999b, 2003). This has been taken as a genetic signature for an “early” (i.e., Middle Paleolithic) colonization of South Asia by modern humans and, consequently, as a confirmation of the “southern route” hypothesis. However, in South Asia, the earliest evidence of modern human industries and remains is dated to ~30,000 years (Kennedy and Deraniyagala 1989; Misra 2001). Because earlier records from Neanderthal-like technologies are found in South Asia (Misra 2001), the absence of modern human traces does not seem to be due to a gap in the archeological record. How to explain a gap of at least 20,000 years long between the archeological and genetic evidence? The coalescence time of haplogroup U in Europe is also dated to 50,000–60,000 years (Richards et al. 2000), but the accepted time scale of the peopling of Europe by modern humans is 35,000–40,000 years ago (Bocquet-Appel and Demars 2000). If so, why should South Asia, but not Europe, be considered to have been colonized 50,000–70,000 years ago? The observed timing discrepancies between genetic and archeological estimates are simply explained by the fact that the divergence of genes predates that of populations (Barbujani et al. 1998). Consequently, the evidence for a Middle Paleolithic colonization of the Indian subcontinent provided by mtDNA studies and the genetic confirmation of the “southern route” hypothesis are all the more debatable.

What conclusive genetic evidence remains for the “southern route” hypothesis? If the Indian subcontinent had received migrants from this putative migration from Africa to the Sahul, it would be reasonable to expect at least some remote affinities between African, Indian, Australian, and/or Papua-New-Guinean (PNG) gene pools. Unfortunately, to date, most studies aimed at detecting traces of an ancient peopling of South Asia through the southern route have not compared the Indian data in a wider context, in particular with people (except Africans) from regions believed to have been involved in the “southern route” migration. Alu insertions data are interpreted as supporting an ancient African-PNG relationship, but India is not a part of this relationship (Stoneking et al. 1997). Y-chromosome and mtDNA data suggest a connection between the Indian subcontinent and Australia, which is, however, dated to <5,000 years (Redd and Stoneking 1999; Redd et al. 2002; Cordaux et al. 2003). Thangaraj et al. (2003) suggest a possible common origin for the inhabitants of the Andaman Islands and PNG, on the basis of high frequencies of mtDNA 16357C in these two areas only. However, we note that 16357C in Andamanese mtDNAs occurs on the background of 16223T and 16319A, corresponding to haplogroup M (Endicott et al. 2003; Thangaraj et al. 2003). In contrast, 16357C in PNG is associated with 16223C and 16319G (Hagelberg et al. 1999; Redd and Stoneking 1999), corresponding to haplogroup N. Consequently, the presence of 16357C in the Andaman Islands and PNG is most likely the result of parallel mutations and not a signature of shared ancestry. In addition, the putative original paternal lineage of Andaman islanders (i.e., Y haplogroup D) (Thangaraj et al. 2003) is not found in Melanesia or Australia (Kayser et al. 2003). Otherwise, mtDNA haplotypes in South Asian ethnic groups are most closely related to east Eurasians and do not show any particular ties to African or PNG populations (Kivisild et al. 2003; Cordaux et al. 2003). In addition, an mtDNA control region motif proposed by Forster et al. (2001) to represent a signature of an early migration from Africa to Sahul through the southern route is not found in South Asia (Cordaux et al. 2003). In summary, there is no convincing support to date for a Middle Paleolithic genetic contribution to South Asia by migrants from Africa to Sahul along the southern route.

If so, and in light of the genetic and archeological evidence, the most reasonable scenario for the peopling of South Asia is an Upper Paleolithic event (i.e., the major expansion of modern humans out of Africa through the Levant [Lahr and Foley 1994]), from which the current Indian gene pool is derived. Proto-Eurasians subsequently evolved to their present distinct South Asian, East Asian, and European gene pools and expanded ~30,000 years ago (Forster et al. 2001). Without requiring a Middle Paleolithic migration of modern humans into South Asia, this scenario explains why (i) most South Asian mtDNA clusters coalesce and show signs of demographic expansions ~30,000 years ago (Kivisild et al. 1999b), (ii) the South Asian mtDNA gene pool is related to (but distinct from) other Eurasian mtDNA pools, (iii) the South Asian mtDNA gene pool does not show close affinities to either Africa or PNG, and (iv) the archeological record does not show evidence for the presence of modern humans in South Asia before ~30,000 years ago. Hypothesizing a Middle Paleolithic migration to South Asia would create more problems than it would solve: it would, in particular, hardly explain the above crucial points iii and iv.

We conclude that there is currently no convincing genetic evidence that supports the postulated Middle Paleolithic migration of modern humans from Africa to the Sahul through South Asia. This does not necessarily mean that such a migration never occurred, since archaeological evidence does document modern humans in Sahul by ~60,000 years ago. However, it is possible that subsequent Upper Paleolithic migrations in Eurasia erased the genetic traces in contemporary populations of this early event in our history (Cavalli-Sforza et al. 1994; Cordaux et al. 2003); in any event, the “southern route” hypothesis still awaits genetic support.

Acknowledgments

We thank David Hughes, Manfred Kayser, Vano Nasidze, and Brigitte Pakendorf for critical reading of the manuscript.

Electronic-Database Information

URLs for data presented herein are as follows:

References

Anderson S, Bankier AT, Barrell BG, De Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich D, Roe B, Sanger F, Schreier P, Smith A, Staden R, Young I (1981) Sequence and organization of the human mitochondrial genome. Nature 290:457–465 [PubMed]
Bamshad M, Kivisild T, Watkins WS, Dixon ME, Ricker CE, Rao BB, Naidu JM, Prasad BVR, Reddy PG, Rasanayagam A, Papiha SS, Villems R, Redd AJ, Hammer MF, Nguyen SV, Carroll ML, Batzer MA, Jorde LB (2001) Genetic evidence on the origins of Indian caste populations. Genome Res 11:994–1004 [PMC free article] [PubMed]
Barbujani G, Bertorelle, G, Chikhi L (1998) Evidence for Paleolithic and Neolithic gene flow in Europe. Am J Hum Genet 62:488–491 [PMC free article] [PubMed]
Bocquet-Appel JP, Demars PY (2000) Neanderthal contraction and modern human colonization of Europe. Antiquity 74:544–552
Cavalli-Sforza LL, Menozzi P, Piazza A (1994) History and geography of human genes. Princeton University Press, Princeton, NJ
Cordaux R, Saha N, Bentley GR, Aunger R, Sirajuddin SM, Stoneking M (2003) Mitochondrial DNA analysis reveals diverse histories of tribal populations from India. Eur J Hum Genet 11:253-264 [PubMed]
Endicott P, Gilbert MTP, Stringer C, Lalueza-Fox C, Willerslev E, Hansen AJ, Cooper A (2003) The genetic origins of Andaman Islanders. Am J Hum Genet 72:178–184 [PMC free article] [PubMed]
Forster P, Torroni A, Renfrew C, Röhl A (2001) Phylogenetic star contraction applied to Asian and Papuan mtDNA evolution. Mol Biol Evol 18:1864–1881 [PubMed]
Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925 [PMC free article] [PubMed]
Hagelberg E, Goldman N, Lio P, Whelan S, Schiefenhövel W, Clegg JB, Bowden DK (1999) Evidence for mitochondrial DNA recombination in a human population of island Melanesia. Proc R Soc Lond Biol Sci 266:485–492 [PMC free article] [PubMed]
Harpending HC, Sherry ST, Rogers AR, Stoneking M (1993) The genetic structure of ancient human populations. Curr Anthropol 34:483–496
Johnson BJ, Miller GH, Fogel ML, Magee JW, Gagan MK, Chivas AR (1999) 65,000 years of vegetation change in central Australia summer monsoon. Science 284:1150–1152 [PubMed]
Kayser M, Brauer S, Weiss G, Schiefenhövel W, Underhill PA, Shen P, Oefner P, Tommaseo-Ponzetta M, Stoneking M (2003) Reduced Y-chromosome, but not mitochondrial DNA, diversity in human populations from West New Guinea. Am J Hum Genet 72:281–302 [PMC free article] [PubMed]
Kennedy KAR, Deraniyagala SU (1989) Fossil remains of 28,000-year-old hominids from Sri Lanka. Curr Anthropol 30:394–399
Kivisild T, Bamshad MJ, Kaldma K, Metspalu E, Reidla M, Laos S, Parik J, Watkins WS, Dixon ME, Papiha SS, Mastana SS, Mir MR, Ferak V, Villems R (1999a) Deep common ancestry of Indian and western-Eurasian mitochondrial DNA lineages. Curr Biol 9:1331–1334 [PubMed]
Kivisild T, Kaldma K, Metspalu M, Parik J, Papiha S, Villems R (1999b) The place of the Indian mitochondrial variants in the global network of maternal lineages and the peopling of the old world. In: Papiha SS, Deka R, Chakraborty R (eds) Genomic diversity: applications in human population genetics. Kluwer Academic/Plenum Publishers, New York, pp 135–152
Kivisild T, Rootsi S, Metspalu M, Mastana S, Kaldma K, Parik J, Metspalu E, Adojaan M, Tolk H-V, Stepanov V, Gölge M, Usanga E, Papiha SS, Cinnioğlu C, King R, Cavalli-Sforza L, Underhill PA, Villems R (2003) The genetic heritage of the earliest settlers persists both in Indian tribal and caste populations. Am J Hum Genet 72:313–332 [PMC free article] [PubMed]
Lahr MM, Foley R (1994) Multiple dispersals and modern humans origins. Evol Anthropol 3:48–60
Meyer S, Weiss G, von Haeseler A (1999) Pattern of nucleotide substitution and rate heterogeneity in the hypervariable regions I and II of human mtDNA. Genetics 152:1103–1110 [PMC free article] [PubMed]
Miller GH, Magee JW, Johnson BJ, Fogel ML, Spooner NA, McCulloch MT, Ayliffe LK (1999) Pleistocene extinction of Genyornis newtoni: human impact on Australian megafauna. Science 283:205–208 [PubMed]
Misra VN (2001) Prehistoric human colonization of India. J Biosci 26:491–531 [PubMed]
Quintana-Murci L, Semino O, Bandelt H-J, Passarino G, McElreavey K, Santachiara-Benerecetti AS (1999) Genetic evidence on an early exit of Homo sapiens sapiens from Africa through eastern Africa. Nat Genet 23:437–441 [PubMed]
Redd AJ, Roberts-Thomson J, Karafet T, Bamshad M, Jorde LB, Naidu JM, Walsh B and Hammer MF (2002) Gene flow from the Indian subcontinent to Australia: evidence from the Y chromosome. Curr Biol 12:673–677 [PubMed]
Redd AJ, Stoneking M (1999) Peopling of Sahul: mtDNA variation in Aboriginal Australian and Papua New Guinean populations. Am J Hum Genet 65:808–828 [PMC free article] [PubMed]
Richards M, Macaulay V, Hickey E, Vega E, Sykes B, Guida V, Rengo C, et al (2000) Tracing European founder lineages in the Near Eastern mtDNA pool. Am J Hum Genet 67:1251–1276 [PMC free article] [PubMed]
Roberts RG, Jones R (1994) Luminescence dating of sediments: new light on the human colonization of Australia. Australian Aboriginal Studies 2:2–17
Stoneking M, Fontius JJ, Clifford SL, Soodyall H, Arcot SS, Saha N, Jenkins T, Tahir MA, Deininger PL, Batzer MA (1997) Alu insertion polymorphisms and human evolution: evidence for a larger population size in Africa. Genome Res 7:1061–1071 [PMC free article] [PubMed]
Stringer CB, Andrews P (1988) Genetic and fossil evidence for the origin of modern humans. Science 239:1263–1268 [PubMed]
Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123:585–595 [PMC free article] [PubMed]
Thangaraj K, Singh L, Reddy AG, Rao VR, Sehgal SC, Underhill PA, Pierson M, Frame IG, Hagelberg E (2003) Genetic affinities of the Andaman Islanders, a vanishing human population. Curr Biol 13:86–93 [PubMed]
Thorne A, Grün R, Mortimer G, Spooner NA, Simpson JJ, McCulloch M, Taylor L, Curnoe D (1999) Australia’s oldest human remains: age of the Lake Mungo 3 skeleton. J Hum Evol 36:591–612 [PubMed]
Underhill PA, Shen P, Lin AA, Jin L, Passarino G, Yang WH, Kauffman E, Bonné-Tamir B, Bertranpetit J, Francalacci P, Ibrahim M, Jenkins T, Kidd JR, Mehdi SQ, Seielstad MT, Spencer Wells R, Piazza A, Davis RW, Feldman MW, Cavalli-Sforza LL, Oefner PJ (2000) Y chromosome sequence variation and the history of human populations. Nat Genet 26:358–361 [PubMed]
Ward RH, Frazier BL, Dew-Jager K, Pääbo S (1991) Extensive mitochondrial diversity within a single Amerindian tribe. Proc Natl Acad Sci USA 88:8720–8724 [PMC free article] [PubMed]

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