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Biochem J. Sep 1970; 119(2): 171–174.
PMCID: PMC1179339

Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture


1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ashworth JM, Watts DJ. Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture. Biochem J. 1970 Sep;119(2):175–182. [PMC free article] [PubMed]
  • Barratt RW, Johnson GB, Ogata WN. Wild-type and mutant stocks of Aspergillus nidulans. Genetics. 1965 Jul;52(1):233–246. [PMC free article] [PubMed]
  • Parker ET, Bernsteinas JB, Green JH. Increased recovery of psychrophilic bacteria by the use of a new medium with lower solidifying temperature. Appl Microbiol. 1968 Nov;16(11):1794–1794. [PMC free article] [PubMed]
  • Reinhardt DJ. Silica gel as a preserving agent for the cellular slime mold Acrasis rosea. J Protozool. 1966 May;13(2):225–226. [PubMed]
  • Wright JA, Maeba P, Sanwal BD. Allosteric regulation of the activity of citrate snythetase of Escherichia coli by alpha-ketoglutarate. Biochem Biophys Res Commun. 1967 Oct 11;29(1):34–38. [PubMed]

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