Logo of biochemjBJ Latest papers and much more!
Biochem J. Mar 1970; 117(1): 49–55.
PMCID: PMC1178828

Separation of antigens by immunological specificity. Studies on the desorption of homologous antigen from disulphide-linked antibody immunosorbents


1. Glycine–hydrochloric acid buffer, pH2.2, desorbed 131I-labelled human serum albumin (100%), lysozyme (100%), ovalbumin (90%), fluorescent ovalbumin (50–60%) and fluorescent human γ-globulin (20%) from their respective homologous disulphide-linked antibody immunosorbents; reasons are suggested for the low recoveries of the fluorescently labelled proteins. 2. Approx. 40% of the recovered 131I-labelled human serum albumin and fluorescent ovalbumin was desorbed above pH6.0, but lysozyme was not eluted until the pH was 3.0 or below. 3. In all cases where high recoveries of antigen were obtained, the immunosorbents could be regenerated and recycled at least four times with full retention of specificity and minimal diminution of capacity. 4. The desorbed antigens were unchanged when compared with the original antigens by quantitative precipitin, specificradioactivity, fluorescent and enzymic analyses and by cellulose acetate electrophoresis. 5. Desorption of antigen with a variety of reagents was investigated. These reagents were less satisfactory than glycine–hydrochloric acid.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Avrameas S, Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies. J Biol Chem. 1967 Apr 10;242(7):1651–1659. [PubMed]
  • Chidlow JW, Stephen J, Smith H. Desorption of antigens from disulphidelinked immunosorbents: recovery of human serum albumin at near neutral pH. Nature. 1968 May 25;218(5143):773–774. [PubMed]
  • Gallop RG, Tozer BT, Stephen J, Smith H. Separation of antigens by immunological specificity: Use of cellulose-linked antibodies as immunosorbents. Biochem J. 1966 Dec;101(3):711–716. [PMC free article] [PubMed]
  • Galvez GE. Specific adsorption of plant viruses by antibodies coupled to a solid matrix. Virology. 1966 Feb;28(2):171–187. [PubMed]
  • GURVICH AE, KAPNER RB, NEZLIN RS. Vydelenie chistykh antitel pri pomoshchi fiksirovannykh na tselliuloze antigenov i izuchenie ikh svoistv. Biokhimiia. 1959 Jan-Feb;24(1):144–156. [PubMed]
  • SMITH H, TOZER BT, GALLOP RC, SCANES FS. Separation of antigens by immunological specificity. 1. Method for separating individual antigen-antibody complexes from mixed antigens and antibodies. Biochem J. 1962 Jul;84:74–80. [PMC free article] [PubMed]
  • Stephen J, Gallop RG, Smith H. Separation of antigens by immunological specificity: Use of disulphide-linked antibodies as immunosorbents. Biochem J. 1966 Dec;101(3):717–720. [PMC free article] [PubMed]
  • Wood KR, Stephen J, Smith H. Specific adsorption of Semliki forest virus on a disulphide-linked immunosorbent derived from rabbit antibody. J Gen Virol. 1968 Mar;2(2):313–316. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...