• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Feb 1972; 126(4): 781–790.
PMCID: PMC1178489

The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid

Abstract

A method is given for small-scale preparation of DNA from 1.0–1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • CHARGAFF E, LIPSHITZ R, GREEN C, HODES ME. The composition of the deoxyribonucleic acid of salmon sperm. J Biol Chem. 1951 Sep;192(1):223–230. [PubMed]
  • Du Buy HG, Mattern CF, Riley FL. Comparison of the DNA's obtained from brain nuclei and mitochondria of mice and from the nuclei and kinetoplasts of Leishmania enriettii. Biochim Biophys Acta. 1966 Aug 17;123(2):298–305. [PubMed]
  • EMANUEL CF, CHAIKOFF IL. Deoxyribonucleic acids of central nervous system, kidney, and spleen: a comparison of some chemical and physical properties. J Neurochem. 1960 May;5:236–244. [PubMed]
  • HERSHEY AD, DIXON J, CHASE M. Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition. J Gen Physiol. 1953 Jul;36(6):777–789. [PMC free article] [PubMed]
  • Jermyn MA, Isherwood FA. Improved separation of sugars on the paper partition chromatogram. Biochem J. 1949;44(4):402–407. [PMC free article] [PubMed]
  • KIRBY KS. Separation of deoxyribonucleic acid from ribonucleic acid without the use of enzymes. Biochim Biophys Acta. 1961 Feb 12;47:18–26. [PubMed]
  • LOEB MR, COHEN SS. The origin of purine and pyrimidine deoxyribose in T6r+ bacteriophage. J Biol Chem. 1959 Feb;234(2):364–369. [PubMed]
  • MARKHAM R, SMITH JD. Chromatographic studies of nucleic acids; a technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances. Biochem J. 1949;45(3):294–298. [PMC free article] [PubMed]
  • Norton WT, Autilio LA. The lipid composition of purified bovine brain myelin. J Neurochem. 1966 Apr;13(4):213–222. [PubMed]
  • Penn NW, Suwalski R. Quantitative determination of deoxyribonucleic acid in rat brain. Biochem J. 1969 Nov;115(3):563–568. [PMC free article] [PubMed]
  • Price PA, Stein WH, Moore S. Effect of divalent cations on the reduction and re-formation of the disulfide bonds of deoxyribonuclease. J Biol Chem. 1969 Feb 10;244(3):929–932. [PubMed]
  • Robinson N. Composition of deoxyribonucleoprotein in human brain. Clin Chim Acta. 1966 Oct;14(4):429–434. [PubMed]
  • SHUGAR D, FOX JJ. Spectrophotometric studies of nucleic acid derivatives and related compounds as a function of pH. I. Pyrimidines. Biochim Biophys Acta. 1952;9(2):199–218. [PubMed]
  • TREVELYAN WE, PROCTER DP, HARRISON JS. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. [PubMed]
  • WYATT GR. The purine and pyrimidine composition of deoxypentose nucleic acids. Biochem J. 1951 May;48(5):584–590. [PMC free article] [PubMed]
  • WYATT GR, COHEN SS. The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine. Biochem J. 1953 Dec;55(5):774–782. [PMC free article] [PubMed]
  • ZAMENHOF S, BURSZTYN H, RICH K, ZAMENHOF PJ. THE DETERMINATION OF DEOXYRIBONUCLEIC ACID AND OF CELL NUMBER IN BRAIN. J Neurochem. 1964 Jul;11:505–509. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...