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Journal List > Biochem J > v.126(4); Feb 1972

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Biochem J. 1972 February; 126(4): 781–790.
PMCID: PMC1178489
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The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid
N. W. Penn,* R. Suwalski, C. O'Riley, K. Bojanowski, and R. Yura†
Neuro-Endocrine Research Unit, Willowbrook State School, New York State Department of Mental Hygiene, Staten Island, N.Y. 10314, U.S.A.
*Present address: c/o Dr. M. M. Rapport, Psychiatric Institute, 722 W. 168th Street, New York, N.Y. 10032, U.S.A. This work was done at the Research Unit, which was closed on 3 June 1971.
†Present address: Hinaga Hospital, Yokkaicho-shi, Mie-ken, Japan.
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Abstract
A method is given for small-scale preparation of DNA from 1.0–1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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