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Biochem J. Dec 1971; 125(4): 1075–1080.
PMCID: PMC1178271

Control of leghaemoglobin synthesis in snake beans

Abstract

1. The finding that the plant is the genetic determinant of leghaemoglobin production in legume nodules was further tested by inoculating snake beans with two strains of Rhizobium selected to give large genetic differences. Carbohydrate requirement patterns, immunological techniques and DNA base ratio determinations were used to demonstrate genetic differences between the two rhizobial strains. 2. Partially purified preparations of the haemoglobins from the nodules produced by the two strains showed no differences when examined by electrophoresis, isoelectric focusing or ion-exchange chromatography. 3. Two different leghaemoglobins from each type of nodule were separated by chromatography on DEAE-cellulose. One of these was isolated in the Fe3+ form and accounted for two-thirds of the total leghaemoglobin. When it was examined in the analytical ultracentrifuge and by amino acid analysis, this major component did not vary with the inoculant rhizobial strain. The molecule had an s20,w of 1.88S, a diffusion coefficient of 10.7×10−7cm2·s−1 and a mol. wt. of 16700. 4. These results strongly support the hypothesis that the mRNA for leghaemoglobin is transcribed from plant DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Appleby CA. Properties of leghaemoglobin in vivo, and its isolation as ferrous oxyleghaemoglobin. Biochim Biophys Acta. 1969;188(2):222–229. [PubMed]
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  • GRAHAM PH. STUDIES ON THE UTILISATION OF CARBOHYDRATES AND KREBS CYCLE INTERMEDIATES BY RHIZOBIA, USING AN AGAR PLATE METHOD. Antonie Van Leeuwenhoek. 1964;30:68–72. [PubMed]
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