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Journal List > BMC Plant Biol > v.5; 2005

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BMC Plant Biol. 2005; 5: 10.
Published online 2005 June 14. doi: 10.1186/1471-2229-5-10.
PMCID: PMC1177970
Copyright © 2005 Wang et al; licensee BioMed Central Ltd.
Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: a new resource for plant comparative genomics
Wenming Wang,1,9 Milos Tanurdzic,2,8 Meizhong Luo,1 Nicholas Sisneros,1 Hye Ran Kim,1 Jing-Ke Weng,3 Dave Kudrna,1 Christopher Mueller,1 K Arumuganathan,4 John Carlson,5 Clint Chapple,3 Claude de Pamphilis,5 Dina Mandoli,6 Jeff Tomkins,7 Rod A Wing,1 and Jo Ann Bankscorresponding author2
1Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
2Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA
3Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA
4Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USA
5Department of Biology and Huck Institutes of Life Sciences, Penn State University, University Park, PA 16802, USA
6Department of Biology and Center for Developmental Biology, University of Washington, Seattle, WA 98195, USA
7Department of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, SC 29634, USA
8Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
9Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
corresponding authorCorresponding author.
Wenming Wang: wange/at/umbi.umd.edu; Milos Tanurdzic: milos/at/cshl.edu; Meizhong Luo: mluo/at/genome.arizona.edu; Nicholas Sisneros: nsisneros/at/genome.arizona.edu; Hye Ran Kim: hkim/at/genome.arizona.edu; Jing-Ke Weng: wengj/at/purdue.edu; Dave Kudrna: dkudrna/at/genome.arizona.edu; Christopher Mueller: cmueller/at/genome.arizona.edu; K Arumuganathan: karu/at/benaroyaresearch.org; John Carlson: jec16/at/psu.edu; Clint Chapple: chapple/at/purdue.edu; Claude de Pamphilis: cwd3/at/psu.edu; Dina Mandoli: mandoli/at/u.washington.edu; Jeff Tomkins: jtmkns/at/clemson.edu; Rod A Wing: rwing/at/Ag.arizona.edu; Jo Ann Banks: banksj/at/purdue.edu
Received January 6, 2005; Accepted June 14, 2005.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Abstract
Background
The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants.
Results
Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.
Conclusion
The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.
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