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EMBO J. 1999 Feb 1; 18(3): 586–594.
PMCID: PMC1171151

Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow.


The fact that substrate-anchored Dictyostelium cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin-bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild-type and, more prominently, in myosin II-null cells. No other F-actin bundling or cross-linking protein tested is co-localized. Green fluorescent protein fusions show that the N-terminal actin-binding domain of cortexillin I is dispensable and the C-terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II-independent system that directs transport of membrane proteins to the cleavage furrow.

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Selected References

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  • Aguado-Velasco C, Bretscher MS. Dictyostelium myosin II null mutant can still cap Con A receptors. Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9684–9686. [PMC free article] [PubMed]
  • Burton K, Taylor DL. Traction forces of cytokinesis measured with optically modified elastic substrata. Nature. 1997 Jan 30;385(6615):450–454. [PubMed]
  • De Lozanne A, Spudich JA. Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science. 1987 May 29;236(4805):1086–1091. [PubMed]
  • Dvorak JA, Nagao E. Kinetic analysis of the mitotic cycle of living vertebrate cells by atomic force microscopy. Exp Cell Res. 1998 Jul 10;242(1):69–74. [PubMed]
  • Faix J, Dittrich W. DGAP1, a homologue of rasGTPase activating proteins that controls growth, cytokinesis, and development in Dictyostelium discoideum. FEBS Lett. 1996 Oct 7;394(3):251–257. [PubMed]
  • Faix J, Steinmetz M, Boves H, Kammerer RA, Lottspeich F, Mintert U, Murphy J, Stock A, Aebi U, Gerisch G. Cortexillins, major determinants of cell shape and size, are actin-bundling proteins with a parallel coiled-coil tail. Cell. 1996 Aug 23;86(4):631–642. [PubMed]
  • Faix J, Clougherty C, Konzok A, Mintert U, Murphy J, Albrecht R, Mühlbauer B, Kuhlmann J. The IQGAP-related protein DGAP1 interacts with Rac and is involved in the modulation of the F-actin cytoskeleton and control of cell motility. J Cell Sci. 1998 Oct;111(Pt 20):3059–3071. [PubMed]
  • Fechheimer M, Murdock D, Carney M, Glover CV. Isolation and sequencing of cDNA clones encoding the Dictyostelium discoideum 30,000-dalton actin-bundling protein. J Biol Chem. 1991 Feb 15;266(5):2883–2889. [PubMed]
  • Fishkind DJ, Wang YL. New horizons for cytokinesis. Curr Opin Cell Biol. 1995 Feb;7(1):23–31. [PubMed]
  • Gerald N, Dai J, Ting-Beall HP, De Lozanne A. A role for Dictyostelium racE in cortical tension and cleavage furrow progression. J Cell Biol. 1998 Apr 20;141(2):483–492. [PMC free article] [PubMed]
  • Glenney JR, Jr, Kaulfus P, Matsudaira P, Weber K. F-actin binding and bundling properties of fimbrin, a major cytoskeletal protein of microvillus core filaments. J Biol Chem. 1981 Sep 10;256(17):9283–9288. [PubMed]
  • Glotzer M. The mechanism and control of cytokinesis. Curr Opin Cell Biol. 1997 Dec;9(6):815–823. [PubMed]
  • Harris AK. Testing cleavage mechanisms by comparing computer simulations to actual experimental results. Ann N Y Acad Sci. 1990;582:60–77. [PubMed]
  • He X, Dembo M. On the mechanics of the first cleavage division of the sea urchin egg. Exp Cell Res. 1997 Jun 15;233(2):252–273. [PubMed]
  • Heim R, Tsien RY. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol. 1996 Feb 1;6(2):178–182. [PubMed]
  • Hiramoto Y. Mechanical properties of the cortex before and during cleavage. Ann N Y Acad Sci. 1990;582:22–30. [PubMed]
  • Jay PY, Elson EL. Surface particle transport mechanism independent of myosin II in Dictyostelium. Nature. 1992 Apr 2;356(6368):438–440. [PubMed]
  • Knecht DA, Loomis WF. Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science. 1987 May 29;236(4805):1081–1086. [PubMed]
  • Koppel DE, Oliver JM, Berlin RD. Surface functions during mitosis. III. Quantitative analysis of ligand-receptor movement into the cleavage furrow: diffusion vs. flow. J Cell Biol. 1982 Jun;93(3):950–960. [PMC free article] [PubMed]
  • Larochelle DA, Vithalani KK, De Lozanne A. A novel member of the rho family of small GTP-binding proteins is specifically required for cytokinesis. J Cell Biol. 1996 Jun;133(6):1321–1329. [PMC free article] [PubMed]
  • Madaule P, Eda M, Watanabe N, Fujisawa K, Matsuoka T, Bito H, Ishizaki T, Narumiya S. Role of citron kinase as a target of the small GTPase Rho in cytokinesis. Nature. 1998 Jul 30;394(6692):491–494. [PubMed]
  • Neujahr R, Heizer C, Gerisch G. Myosin II-independent processes in mitotic cells of Dictyostelium discoideum: redistribution of the nuclei, re-arrangement of the actin system and formation of the cleavage furrow. J Cell Sci. 1997 Jan;110(Pt 2):123–137. [PubMed]
  • Neujahr R, Heizer C, Albrecht R, Ecke M, Schwartz JM, Weber I, Gerisch G. Three-dimensional patterns and redistribution of myosin II and actin in mitotic Dictyostelium cells. J Cell Biol. 1997 Dec 29;139(7):1793–1804. [PMC free article] [PubMed]
  • Neujahr R, Albrecht R, Köhler J, Matzner M, Schwartz JM, Westphal M, Gerisch G. Microtubule-mediated centrosome motility and the positioning of cleavage furrows in multinucleate myosin II-null cells. J Cell Sci. 1998 May;111(Pt 9):1227–1240. [PubMed]
  • Rivero F, Furukawa R, Noegel AA, Fechheimer M. Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy. J Cell Biol. 1996 Nov;135(4):965–980. [PMC free article] [PubMed]
  • Rivero F, Köppel B, Peracino B, Bozzaro S, Siegert F, Weijer CJ, Schleicher M, Albrecht R, Noegel AA. The role of the cortical cytoskeleton: F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development. J Cell Sci. 1996 Nov;109(Pt 11):2679–2691. [PubMed]
  • Satterwhite LL, Pollard TD. Cytokinesis. Curr Opin Cell Biol. 1992 Feb;4(1):43–52. [PubMed]
  • Simson R, Wallraff E, Faix J, Niewöhner J, Gerisch G, Sackmann E. Membrane bending modulus and adhesion energy of wild-type and mutant cells of Dictyostelium lacking talin or cortexillins. Biophys J. 1998 Jan;74(1):514–522. [PMC free article] [PubMed]
  • Steinmetz MO, Stock A, Schulthess T, Landwehr R, Lustig A, Faix J, Gerisch G, Aebi U, Kammerer RA. A distinct 14 residue site triggers coiled-coil formation in cortexillin I. EMBO J. 1998 Apr 1;17(7):1883–1891. [PMC free article] [PubMed]
  • Tuxworth RI, Cheetham JL, Machesky LM, Spiegelmann GB, Weeks G, Insall RH. Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. J Cell Biol. 1997 Aug 11;138(3):605–614. [PMC free article] [PubMed]
  • Watts DJ, Ashworth JM. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970 Sep;119(2):171–174. [PMC free article] [PubMed]
  • Weber I, Wallraff E, Albrecht R, Gerisch G. Motility and substratum adhesion of Dictyostelium wild-type and cytoskeletal mutant cells: a study by RICM/bright-field double-view image analysis. J Cell Sci. 1995 Apr;108(Pt 4):1519–1530. [PubMed]
  • White JG. Laterally mobile, cortical tension elements can self-assemble into a contractile ring. Ann N Y Acad Sci. 1990;582:50–59. [PubMed]
  • White JG, Borisy GG. On the mechanisms of cytokinesis in animal cells. J Theor Biol. 1983 Mar 21;101(2):289–316. [PubMed]
  • Nossal GJ. The molecular and cellular basis of affinity maturation in the antibody response. Cell. 1992 Jan 10;68(1):1–2. [PubMed]
  • Wolpert L. The mechanical properties of the membrane of the sea urchin egg during cleavage. Exp Cell Res. 1966 Feb;41(2):385–396. [PubMed]
  • Zang JH, Cavet G, Sabry JH, Wagner P, Moores SL, Spudich JA. On the role of myosin-II in cytokinesis: division of Dictyostelium cells under adhesive and nonadhesive conditions. Mol Biol Cell. 1997 Dec;8(12):2617–2629. [PMC free article] [PubMed]

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