• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Nov 16, 1998; 17(22): 6449–6464.
PMCID: PMC1170993

A novel complex of membrane proteins required for formation of a spherical nucleus.


Two membrane proteins were identified through their genetic interaction with the nucleoporin Nup84p and shown to participate in nuclear envelope morphogenesis in yeast. One component is a known sporulation factor Spo7p, and the other, Nem1p, a novel protein whose C-terminal domain is conserved during eukaryotic evolution. Spo7p and Nem1p localize to the nuclear/ER membrane and behave biochemically as integral membrane proteins. Nem1p binds to Spo7p via its conserved C-terminal domain. Although cells without Spo7p or Nem1p are viable, they exhibit a drastically altered nuclear morphology with long, pore-containing double nuclear membrane extensions. These protrusions emanate from a core nucleus which contains the DNA, and penetrate deeply into the cytoplasm. Interestingly, not only Spo7(-) and Nem1(-), but also several nucleoporin mutants are defective in sporulation. Thus, Spo7p and Nem1p, which exhibit a strong genetic link to nucleoporins of the Nup84p complex, fulfil an essential role in formation of a spherical nucleus and meiotic division.

Full Text

The Full Text of this article is available as a PDF (970K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aitchison JD, Rout MP, Marelli M, Blobel G, Wozniak RW. Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p. J Cell Biol. 1995 Dec;131(5):1133–1148. [PMC free article] [PubMed]
  • Belgareh N, Doye V. Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells. J Cell Biol. 1997 Feb 24;136(4):747–759. [PMC free article] [PubMed]
  • Bergès T, Petfalski E, Tollervey D, Hurt EC. Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification. EMBO J. 1994 Jul 1;13(13):3136–3148. [PMC free article] [PubMed]
  • Bogerd AM, Hoffman JA, Amberg DC, Fink GR, Davis LI. nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. J Cell Biol. 1994 Oct;127(2):319–332. [PMC free article] [PubMed]
  • Bucci M, Wente SR. In vivo dynamics of nuclear pore complexes in yeast. J Cell Biol. 1997 Mar 24;136(6):1185–1199. [PMC free article] [PubMed]
  • Doye V, Hurt E. From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol. 1997 Jun;9(3):401–411. [PubMed]
  • Doye V, Wepf R, Hurt EC. A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO J. 1994 Dec 15;13(24):6062–6075. [PMC free article] [PubMed]
  • Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene. 1988 Oct 30;70(2):303–312. [PubMed]
  • Esposito MS, Esposito RE. The genetic control of sporulation in Saccharomyces. I. The isolation of temperature-sensitive sporulation-deficient mutants. Genetics. 1969 Jan;61(1):79–89. [PMC free article] [PubMed]
  • Esposito MS, Esposito RE. Genes controlling meiosis and spore formation in yeast. Genetics. 1974 Sep;78(1):215–225. [PMC free article] [PubMed]
  • Gant TM, Wilson KL. Nuclear assembly. Annu Rev Cell Dev Biol. 1997;13:669–695. [PubMed]
  • Gietz RD, Sugino A. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. 1988 Dec 30;74(2):527–534. [PubMed]
  • Goldberg MW, Allen TD. Structural and functional organization of the nuclear envelope. Curr Opin Cell Biol. 1995 Jun;7(3):301–309. [PubMed]
  • Goldberg MW, Wiese C, Allen TD, Wilson KL. Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly. J Cell Sci. 1997 Feb;110(Pt 4):409–420. [PubMed]
  • Goldstein AL, Snay CA, Heath CV, Cole CN. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol Biol Cell. 1996 Jun;7(6):917–934. [PMC free article] [PubMed]
  • Grandi P, Doye V, Hurt EC. Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J. 1993 Aug;12(8):3061–3071. [PMC free article] [PubMed]
  • Hellmuth K, Lau DM, Bischoff FR, Künzler M, Hurt E, Simos G. Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol Cell Biol. 1998 Nov;18(11):6374–6386. [PMC free article] [PubMed]
  • Ho AK, Raczniak GA, Ives EB, Wente SR. The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function. Mol Biol Cell. 1998 Feb;9(2):355–373. [PMC free article] [PubMed]
  • Hurt EC. A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J. 1988 Dec 20;7(13):4323–4334. [PMC free article] [PubMed]
  • Ishikawa S, Kai M, Tamari M, Takei Y, Takeuchi K, Bandou H, Yamane Y, Ogawa M, Nakamura Y. Sequence analysis of a 685-kb genomic region on chromosome 3p22-p21.3 that is homozygously deleted in a lung carcinoma cell line. DNA Res. 1997 Feb 28;4(1):35–43. [PubMed]
  • Jordan EG, Severs NJ, Williamson DH. Nuclear pore formation and the cell cycle in Saccharomyces cerevisiae. Exp Cell Res. 1977 Feb;104(2):446–449. [PubMed]
  • Kranz JE, Holm C. Cloning by function: an alternative approach for identifying yeast homologs of genes from other organisms. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6629–6633. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Moens PB, Esposito RE, Esposito MS. Aberrant nuclear behavior at meiosis and anucleate spore formation by sporulation-deficient (SPO) mutants of Saccharomyces cerevisiae. Exp Cell Res. 1974 Jan;83(1):166–174. [PubMed]
  • Monier S, Parton RG, Vogel F, Behlke J, Henske A, Kurzchalia TV. VIP21-caveolin, a membrane protein constituent of the caveolar coat, oligomerizes in vivo and in vitro. Mol Biol Cell. 1995 Jul;6(7):911–927. [PMC free article] [PubMed]
  • Nehrbass U, Rout MP, Maguire S, Blobel G, Wozniak RW. The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex. J Cell Biol. 1996 Jun;133(6):1153–1162. [PMC free article] [PubMed]
  • Neiman AM. Prospore membrane formation defines a developmentally regulated branch of the secretory pathway in yeast. J Cell Biol. 1998 Jan 12;140(1):29–37. [PMC free article] [PubMed]
  • Panté N, Aebi U. Toward a molecular understanding of the structure and function of the nuclear pore complex. Int Rev Cytol. 1995;162B:225–255. [PubMed]
  • Parrish ML, Sengstag C, Rine JD, Wright RL. Identification of the sequences in HMG-CoA reductase required for karmellae assembly. Mol Biol Cell. 1995 Nov;6(11):1535–1547. [PMC free article] [PubMed]
  • Pemberton LF, Rout MP, Blobel G. Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes. Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1187–1191. [PMC free article] [PubMed]
  • Preuss D, Mulholland J, Kaiser CA, Orlean P, Albright C, Rose MD, Robbins PW, Botstein D. Structure of the yeast endoplasmic reticulum: localization of ER proteins using immunofluorescence and immunoelectron microscopy. Yeast. 1991 Dec;7(9):891–911. [PubMed]
  • Pryer NK, Salama NR, Schekman R, Kaiser CA. Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro. J Cell Biol. 1993 Feb;120(4):865–875. [PMC free article] [PubMed]
  • Rout MP, Blobel G. Isolation of the yeast nuclear pore complex. J Cell Biol. 1993 Nov;123(4):771–783. [PMC free article] [PubMed]
  • Schneiter R, Hitomi M, Ivessa AS, Fasch EV, Kohlwein SD, Tartakoff AM. A yeast acetyl coenzyme A carboxylase mutant links very-long-chain fatty acid synthesis to the structure and function of the nuclear membrane-pore complex. Mol Cell Biol. 1996 Dec;16(12):7161–7172. [PMC free article] [PubMed]
  • Segref A, Sharma K, Doye V, Hellwig A, Huber J, Lührmann R, Hurt E. Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J. 1997 Jun 2;16(11):3256–3271. [PMC free article] [PubMed]
  • Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem. 1996 Mar 1;68(5):850–858. [PubMed]
  • Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989 May;122(1):19–27. [PMC free article] [PubMed]
  • Siniossoglou S, Wimmer C, Rieger M, Doye V, Tekotte H, Weise C, Emig S, Segref A, Hurt EC. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell. 1996 Jan 26;84(2):265–275. [PubMed]
  • Smith S, Blobel G. Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10124–10128. [PMC free article] [PubMed]
  • Stotz A, Linder P. The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors. Gene. 1990 Oct 30;95(1):91–98. [PubMed]
  • Su YA, Lee MM, Hutter CM, Meltzer PS. Characterization of a highly conserved gene (OS4) amplified with CDK4 in human sarcomas. Oncogene. 1997 Sep;15(11):1289–1294. [PubMed]
  • Teixeira MT, Siniossoglou S, Podtelejnikov S, Bénichou JC, Mann M, Dujon B, Hurt E, Fabre E. Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. EMBO J. 1997 Aug 15;16(16):5086–5097. [PMC free article] [PubMed]
  • Vergères G, Yen TS, Aggeler J, Lausier J, Waskell L. A model system for studying membrane biogenesis. Overexpression of cytochrome b5 in yeast results in marked proliferation of the intracellular membrane. J Cell Sci. 1993 Sep;106(Pt 1):249–259. [PubMed]
  • Wente SR, Blobel G. A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic. J Cell Biol. 1993 Oct;123(2):275–284. [PMC free article] [PubMed]
  • Wente SR, Blobel G. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J Cell Biol. 1994 Jun;125(5):955–969. [PMC free article] [PubMed]
  • Wente SR, Rout MP, Blobel G. A new family of yeast nuclear pore complex proteins. J Cell Biol. 1992 Nov;119(4):705–723. [PMC free article] [PubMed]
  • Whyte W, Keopp LH, Lamb J, Crowley JC, Kaback DB. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene. Gene. 1990 Oct 30;95(1):65–72. [PubMed]
  • Wilm M, Mann M. Analytical properties of the nanoelectrospray ion source. Anal Chem. 1996 Jan 1;68(1):1–8. [PubMed]
  • Wimmer C, Doye V, Grandi P, Nehrbass U, Hurt EC. A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1. EMBO J. 1992 Dec;11(13):5051–5061. [PMC free article] [PubMed]
  • Winey M, Hoyt MA, Chan C, Goetsch L, Botstein D, Byers B. NDC1: a nuclear periphery component required for yeast spindle pole body duplication. J Cell Biol. 1993 Aug;122(4):743–751. [PMC free article] [PubMed]
  • Winey M, Yarar D, Giddings TH, Jr, Mastronarde DN. Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes. Mol Biol Cell. 1997 Nov;8(11):2119–2132. [PMC free article] [PubMed]
  • Wozniak RW, Blobel G, Rout MP. POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope. J Cell Biol. 1994 Apr;125(1):31–42. [PMC free article] [PubMed]
  • Wright R, Basson M, D'Ari L, Rine J. Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus. J Cell Biol. 1988 Jul;107(1):101–114. [PMC free article] [PubMed]
  • Yang Q, Rout MP, Akey CW. Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell. 1998 Jan;1(2):223–234. [PubMed]
  • Zabel U, Doye V, Tekotte H, Wepf R, Grandi P, Hurt EC. Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p. J Cell Biol. 1996 Jun;133(6):1141–1152. [PMC free article] [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Conserved Domains
    Conserved Domains
    Link to related CDD entry
  • Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene links
  • MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Published protein sequences
  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...