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J Anat. Feb 1996; 188(Pt 1): 87–95.
PMCID: PMC1167636

Normal and healing ligament vascularity: a quantitative histological assessment in the adult rabbit medial collateral ligament.


Normal and healing adult rabbit medial collateral ligaments (MCL) have been assessed for microvascular anatomy using a quantitative image analysis methodology. MCL preparation by ink-gelatin perfusion enabled acceptable visualisation of microvascular channels within the tissue. Fifteen adult rabbits were studied; 3 normal rabbits formed an external time-zero control group; 12 animals received a standardised gap injury to the right MCL. The ligament injury was permitted to heal for 3, 6, 17 or 40 wk (3 animals in each healing group). Results confirmed that the normal MCL is hypovascular (about 1.46% vascularity by area) and that microvascular channels are highly organised and oriented longitudinally deep within the tissue. Healing MCL scar becomes twice as vascular as normal ligament early on, but returns to near normal values by 40 wk. Microvascular channels appear less organised in scar than in contralateral controls, but remodel with time. The directional scatter and spatial extent of ligament microvascular channels is quantifiable in normal and healing tissues.

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  • Alm A, Strömberg B. Vascular anatomy of the patellar and cruciate ligaments. A microangiographic and histologic investigation in the dog. Acta Chir Scand Suppl. 1974;445:25–35. [PubMed]
  • Arnoczky SP. Blood supply to the anterior cruciate ligament and supporting structures. Orthop Clin North Am. 1985 Jan;16(1):15–28. [PubMed]
  • Arnoczky SP, Rubin RM, Marshall JL. Microvasculature of the cruciate ligaments and its response to injury. An experimental study in dogs. J Bone Joint Surg Am. 1979 Dec;61(8):1221–1229. [PubMed]
  • Arnoczky SP. Anatomy of the anterior cruciate ligament. Clin Orthop Relat Res. 1983 Jan-Feb;(172):19–25. [PubMed]
  • Arnoczky SP, Tarvin GB, Marshall JL. Anterior cruciate ligament replacement using patellar tendon. An evaluation of graft revascularization in the dog. J Bone Joint Surg Am. 1982 Feb;64(2):217–224. [PubMed]
  • Bray RC, Fisher AW, Frank CB. Fine vascular anatomy of adult rabbit knee ligaments. J Anat. 1990 Oct;172:69–79. [PMC free article] [PubMed]
  • BROOKES M, HARRISON RG. The vascularization of the rabbit femur and tibio-fibula. J Anat. 1957 Jan;91(1):61–72. [PMC free article] [PubMed]
  • Carr AJ, Norris SH. The blood supply of the calcaneal tendon. J Bone Joint Surg Br. 1989 Jan;71(1):100–101. [PubMed]
  • Chimich D, Frank C, Shrive N, Dougall H, Bray R. The effects of initial end contact on medial collateral ligament healing: a morphological and biomechanical study in a rabbit model. J Orthop Res. 1991 Jan;9(1):37–47. [PubMed]
  • Chowdhury P, Matyas JR, Frank CB. The "epiligament" of the rabbit medial collateral ligament: a quantitative morphological study. Connect Tissue Res. 1991;27(1):33–50. [PubMed]
  • Dunlap J, McCarthy JA, Joyce ME, Ogata K, Shively RA. Quantification of the perfusion of the anterior cruciate ligament and the effects of stress and injury to supporting structures. Am J Sports Med. 1989 Nov-Dec;17(6):808–810. [PubMed]
  • Eng K, Rangayyan RM, Bray RC, Frank CB, Anscomb L, Veale P. Quantitative analysis of the fine vascular anatomy of articular ligaments. IEEE Trans Biomed Eng. 1992 Mar;39(3):296–306. [PubMed]
  • Eriksdotter-Nilsson M, Björklund H, Olson L. Laminin immunohistochemistry: a simple method to visualize and quantitate vascular structures in the mammalian brain. J Neurosci Methods. 1986 Sep;17(4):275–286. [PubMed]
  • Frank CB, Loitz B, Bray R, Chimich D, King G, Shrive N. Abnormality of the contralateral ligament after injuries of the medial collateral ligament. An experimental study in rabbits. J Bone Joint Surg Am. 1994 Mar;76(3):403–412. [PubMed]
  • Knight AD, Levick JR. The density and distribution of capillaries around a synovial cavity. Q J Exp Physiol. 1983 Oct;68(4):629–644. [PubMed]
  • McFarland EG, Morrey BF, An KN, Wood MB. The relationship of vascularity and water content to tensile strength in a patellar tendon replacement of the anterior cruciate in dogs. Am J Sports Med. 1986 Nov-Dec;14(6):436–448. [PubMed]
  • Proia AD, Chandler DB, Haynes WL, Smith CF, Suvarnamani C, Erkel FH, Klintworth GK. Quantitation of corneal neovascularization using computerized image analysis. Lab Invest. 1988 Apr;58(4):473–479. [PubMed]
  • Scapinelli R. Studies on the vasculature of the human knee joint. Acta Anat (Basel) 1968;70(3):305–331. [PubMed]
  • Simkin PA, Huang A, Benedict RS. Effects of exercise on blood flow to canine articular tissues. J Orthop Res. 1990 Mar;8(2):297–303. [PubMed]
  • Weiss HR. Measurement of cerebral capillary perfusion with a fluorescent label. Microvasc Res. 1988 Sep;36(2):172–180. [PubMed]

Articles from Journal of Anatomy are provided here courtesy of Anatomical Society of Great Britain and Ireland


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