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EMBO J. Jul 1986; 5(7): 1595–1600.
PMCID: PMC1166984

Granules of cytolytic T-lymphocytes contain two serine esterases.

Abstract

Cytoplasmic granules from cytolytic T-lymphocytes (CTL) contain two proteins which react with the serine esterase-specific affinity label diisopropylfluorophosphate (DFP). One of these is a trypsin-like esterase which consists of two disulfide-linked 35-kd subunits. The other consists of a single 29-kd chain. Both molecules are induced concomitantly with cytolytic activity and perforin activity in a CTL-derived T-cell hybrid.

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  • Borregaard N, Heiple JM, Simons ER, Clark RA. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation. J Cell Biol. 1983 Jul;97(1):52–61. [PMC free article] [PubMed]
  • Chang TW, Eisen HN. Effects of N alpha-tosyl-L-lysyl-chloromethylketone on the activity of cytotoxic T lymphocytes. J Immunol. 1980 Mar;124(3):1028–1033. [PubMed]
  • Conzelmann A, Corthésy P, Cianfriglia M, Silva A, Nabholz M. Hybrids between rat lymphoma and mouse T cells with inducible cytolytic activity. Nature. 1982 Jul 8;298(5870):170–172. [PubMed]
  • Coombs GH. Proteinases of Leishmania mexicana and other flagellate protozoa. Parasitology. 1982 Feb;84(1):149–155. [PubMed]
  • Erard F, Corthesy P, Smith KA, Fiers W, Conzelmann A, Nabholz M. Characterization of soluble factors that induce the cytolytic activity and the expression of T cell growth factor receptors of a T cell hybrid. J Exp Med. 1984 Aug 1;160(2):584–599. [PMC free article] [PubMed]
  • Henkart PA. Mechanism of lymphocyte-mediated cytotoxicity. Annu Rev Immunol. 1985;3:31–58. [PubMed]
  • Henkart PA, Millard PJ, Reynolds CW, Henkart MP. Cytolytic activity of purified cytoplasmic granules from cytotoxic rat large granular lymphocyte tumors. J Exp Med. 1984 Jul 1;160(1):75–93. [PMC free article] [PubMed]
  • Hudig D, Redelman D, Minning LL. The requirement for proteinase activity for human lymphocyte-mediated natural cytotoxicity (NK): evidence that the proteinase is serine dependent and has aromatic amino acid specificity of cleavage. J Immunol. 1984 Nov;133(5):2647–2654. [PubMed]
  • Lavie G, Leib Z, Servadio C. The mechanism of human NK cell-mediated cytotoxicity. Mode of action of surface-associated proteases in the early stages of the lytic reaction. J Immunol. 1985 Aug;135(2):1470–1476. [PubMed]
  • Marx JL. How killer cells kill their targets. Science. 1986 Mar 21;231(4744):1367–1369. [PubMed]
  • Masson D, Tschopp J. Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes. J Biol Chem. 1985 Aug 5;260(16):9069–9072. [PubMed]
  • Masson D, Corthésy P, Nabholz M, Tschopp J. Appearance of cytolytic granules upon induction of cytolytic activity in CTL-hybrids. EMBO J. 1985 Oct;4(10):2533–2538. [PMC free article] [PubMed]
  • Millard PJ, Henkart MP, Reynolds CW, Henkart PA. Purification and properties of cytoplasmic granules from cytotoxic rat LGL tumors. J Immunol. 1984 Jun;132(6):3197–3204. [PubMed]
  • Pasternack MS, Eisen HN. A novel serine esterase expressed by cytotoxic T lymphocytes. Nature. 314(6013):743–745. [PubMed]
  • Petty HR, Hermann W, Dereski W, Frey T, McConnell HM. Activatable esterase activity of murine natural killer cell--YAC tumour cell conjugates. J Cell Sci. 1984 Dec;72:1–13. [PubMed]
  • Pierzchala PA, Dorn CP, Zimmerman M. A new fluorogenic substrate for plasmin. Biochem J. 1979 Dec 1;183(3):555–559. [PMC free article] [PubMed]
  • Podack ER, Konigsberg PJ. Cytolytic T cell granules. Isolation, structural, biochemical, and functional characterization. J Exp Med. 1984 Sep 1;160(3):695–710. [PMC free article] [PubMed]
  • Podack ER, Young JD, Cohn ZA. Isolation and biochemical and functional characterization of perforin 1 from cytolytic T-cell granules. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8629–8633. [PMC free article] [PubMed]
  • Redelman D, Hudig D. The mechanism of cell-mediated cytotoxicity. III. Protease-specific inhibitors preferentially block later events in cytotoxic T lymphocyte-mediated lysis than do inhibitors of methylation or thiol-reactive agents. Cell Immunol. 1983 Oct 1;81(1):9–21. [PubMed]
  • Scheiner CJ, Quigley JP. Detection, identification, and partial characterization of serine proteases and esterases in biological systems. Anal Biochem. 1982 May 1;122(1):58–69. [PubMed]
  • Sekaly RP, MacDonald HR, Zaech P, Nabholz M. Cell cycle regulation of cloned cytolytic T cells by T cell growth factor: analysis by flow microfluorometry. J Immunol. 1982 Oct;129(4):1407–1414. [PubMed]

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