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Biochem J. Jan 1974; 137(1): 143–144.
PMCID: PMC1166095

A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors (Short Communication)

Abstract

A new method is described for plotting kinetic results for inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K′i, of an uncompetitive, mixed or non-competitive inhibitor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. [PMC free article] [PubMed]
  • DOWD JE, RIGGS DS. A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS. J Biol Chem. 1965 Feb;240:863–869. [PubMed]
  • JOHANSEN G, LUMRY R. Statistical analysis of enzymic steady-state rate data. C R Trav Lab Carlsberg. 1961;32:185–214. [PubMed]
  • Schlamowitz M, Shaw A, Jackson WT. Limitations of he Dixon plot for ascertaining naure of enzyme inhibition. Tex Rep Biol Med. 1969 Summer;27(2):483–488. [PubMed]
  • WILKINSON GN. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. [PMC free article] [PubMed]

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