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Biochem J. Dec 1973; 135(4): 805–814.
PMCID: PMC1165898

The thermal denaturation of human oxyhaemoglobins A, A2, C and S


1. The time-courses of thermal denaturation of human oxyhaemoglobins A, A2, C and S at 45°C were studied by following the increase in protein fluorescence. Haemoglobins S and C were less stable than haemoglobin A, whereas haemoglobin A2 was considerably more stable. 2. The time-courses of denaturation did not follow first-order kinetics and could be fitted most simply to a co-operative scheme in which the partial denaturation of the α chain preceded that of the β chain. 3. The denaturation of these haemoglobins was studied as a function of temperature by using optical rotatory dispersion. Haemoglobin A2 was again more stable than the others. The addition of small quantities of haemoglobin A2 had a disproportionate effect on the stability of haemoglobin C. 4. The thermodynamic parameters of the denaturation process were calculated.

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Selected References

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  • Acampora G, Hermans J., Jr Reversible denaturation of sperm whale myoglobin. I. Dependence on temperature, pH, and composition. J Am Chem Soc. 1967 Mar 29;89(7):1543–1547. [PubMed]
  • Cassoly R, Bucci E, Iwatsubo M, Banerjee R. Functional studies on human semi-hemoglobin. Biochim Biophys Acta. 1967 Apr 11;133(3):557–567. [PubMed]
  • Chiancone E. Dissociation of hemoglobin into subunits. II. Human oxyhemoglobin: gel filtration studies. J Biol Chem. 1968 Mar 25;243(6):1212–1219. [PubMed]
  • CRADOCK-WATSON JE, FENTON JC, LEHMANN H. Tris buffer for the demonstration of haemoglobin A2 by paper electrophoresis. J Clin Pathol. 1959 Jul;12:372–373. [PMC free article] [PubMed]
  • Dixon HB, McIntosh R. Reduction of methaemoglobin in haemoglobin samples using gel filtration for continuous removal of reaction products. Nature. 1967 Jan 28;213(5074):399–400. [PubMed]
  • GRAHAM JL, GRUNBAUM BW. A rapid method for microelectrophoresis and quantitation of hemoglobins on cellulose acetate. Am J Clin Pathol. 1963 Jun;39:567–578. [PubMed]
  • Guidotti G. Studies on the chemistry of hemoglobin. II. The effect of salts on the dissociation of hemoglobin into subunits. J Biol Chem. 1967 Aug 25;242(16):3685–3693. [PubMed]
  • Lehmann H, Carrell RW. Differences between alpha- and beta-chain mutants of human haemoglobin and between alpha- and beta-thalassaemia. Possible duplication of the alpha-chain gene. Br Med J. 1968 Dec 21;4(5633):748–750. [PMC free article] [PubMed]
  • Perutz MF, Lehmann H. Molecular pathology of human haemoglobin. Nature. 1968 Aug 31;219(5157):902–909. [PubMed]
  • PERUTZ MF, MITCHISON JM. State of haemoglobin in sickle-cell anaemia. Nature. 1950 Oct 21;166(4225):677–679. [PubMed]
  • Perutz MF, Muirhead H, Cox JM, Goaman LC. Three-dimensional Fourier synthesis of horse oxyhaemoglobin at 2.8 A resolution: the atomic model. Nature. 1968 Jul 13;219(5150):131–139. [PubMed]
  • Steinhardt J, Polet H, Moezie F. Acid denaturation of horse carbonylhemoglobin in the absence of oxygen. J Biol Chem. 1966 Sep 10;241(17):3988–3996. [PubMed]
  • Tanford C. Protein denaturation. Adv Protein Chem. 1968;23:121–282. [PubMed]
  • TEALE FW. The ultraviolet fluorescence of proteins in neutral solution. Biochem J. 1960 Aug;76:381–388. [PMC free article] [PubMed]
  • Wrightstone RN, Huisman TH, van der Sar A. Qualitative and quantitative studies of sickle cell hemoglobin in homozygotes and heterozygotes. Clin Chim Acta. 1968 Dec;22(4):593–601. [PubMed]

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