• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Dec 1973; 135(4): 631–637.
PMCID: PMC1165877

The molecular-weight distribution of glycosaminoglycans

Abstract

1. A rapid and sensitive method for the accurate estimation of the molecular-weight distribution of keratan sulphate and chondroitin sulphate isolated from adult bovine nasal septum and intervertebral disc is described. The method utilizes gel chromatography of reductively labelled glycosaminoglycan and end-group estimation of number-average molecular weight for each fraction across the peak of eluted glycosaminoglycan. 2. Chain-length distribution data obtained by this procedure are used to evaluate mechanisms of chondroitin sulphate biosynthesis.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1002K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anseth A, Antonopoulos CA, Bjelle A, Fransson LA. Fractionation and quantitative determination of keratan sulfate using cetylpyridinium chloride and ECTEOLA-cellulose. Biochim Biophys Acta. 1970 Sep 22;215(3):522–526. [PubMed]
  • ANTONOPOULOS CA, BORELIUS E, GARDELL S, HAMNSTROM B, SCOTT JE. The precipitation of polyanions by long-chain aliphatic ammonium compounds. IV. Elution in salt solutions of mucopolysaccharide-quaternary ammonium complexes adsorbed on a support. Biochim Biophys Acta. 1961 Dec 9;54:213–226. [PubMed]
  • BLUMBERG BS, OGSTON AG. The effects of proteolytic enzymes on the hyaluronic acid complex of ox synovial fluid. Biochem J. 1957 Jun;66(2):342–346. [PMC free article] [PubMed]
  • BLUMBERG BS, OGSTON AG. Further evidence on the protein complexes of some hyaluronic acids. Biochem J. 1958 Jan;68(1):183–188. [PMC free article] [PubMed]
  • Constantopoulos G, Dekaban AS, Carroll WR. Determination of molecular weight distribution of acid mucopolysaccharides by sephadex gel filtration. Anal Biochem. 1969 Oct 1;31(1):59–70. [PubMed]
  • Hjertquist SO, Lemperg R. Identification and concentration of the glycosaminoglycans of human articular cartilage in relation to age and osteoarthritis. Calcif Tissue Res. 1972;10(3):223–237. [PubMed]
  • Hjertquist SO, Wasteson A. The molecular weight of chondroitin sulphate from human articular cartilage. Effect of age and of osteoarthritis. Calcif Tissue Res. 1972;10(1):31–37. [PubMed]
  • Hoffman P, Mashburn TA, Jr, Meyer K. Proteinpolysaccharide of bovine cartilage. II. The relation of keratan sulfate and chondroitin sulfate. J Biol Chem. 1967 Sep 10;242(17):3805–3809. [PubMed]
  • Laurent TC, Granath KA. Fractionation of dextran and Ficoll by chromatography on Sephadex G-200. Biochim Biophys Acta. 1967 Mar 22;136(2):191–198. [PubMed]
  • Luscombe M, Phelps CF. Action of degradative enzymes on the light fraction of bovine septa protein polysaccharide. Biochem J. 1967 Apr;103(1):103–109. [PMC free article] [PubMed]
  • Marler E, Davidson EA. Structure of a polysaccharide protein complex. Proc Natl Acad Sci U S A. 1965 Aug;54(2):648–656. [PMC free article] [PubMed]
  • Mason RM, Wusteman FS. The glycosaminoglycans of human tracheobronchial cartilage. Biochem J. 1970 Dec;120(4):777–785. [PMC free article] [PubMed]
  • Mathews MB. Comparative biochemistry of chondroitin sulphate-proteins of cartilage and notochord. Biochem J. 1971 Nov;125(1):37–46. [PMC free article] [PubMed]
  • Robinson HC, Dorfman A. The sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses. J Biol Chem. 1969 Jan 25;244(2):348–352. [PubMed]
  • Robinson HC, Hopwood JJ. The alkaline cleavage and borohydride reduction of cartilage proteoglycan. Biochem J. 1973 Jul;133(3):457–470. [PMC free article] [PubMed]
  • Telser A, Robinson HC, Dorfman A. The biosynthesis of chondroitin-sulfate protein complex. Proc Natl Acad Sci U S A. 1965 Sep;54(3):912–919. [PMC free article] [PubMed]
  • TREVELYAN WE, HARRISON JS. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. [PMC free article] [PubMed]
  • Wasteson A. A method for the determination of molecular weight dispersion in chondroitin sulphate on a microgram level. Biochim Biophys Acta. 1969 Feb 18;177(1):152–154. [PubMed]
  • Wasteson A. A method for the determination of the molecular weight and molecular-weight distribution of chondroitin sulphate. J Chromatogr. 1971 Jul 8;59(1):87–97. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...