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Biochem J. 1980 Jun 1; 187(3): 909–912.
PMCID: PMC1162479

Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates.


Benzyloxycarbonyl-phenylalanyl-arginine 4-methyl-7-coumarylamide was found to be an excellent substrate for the fluorimetric assay of cathepsin B, and arginine 4-methyl-7-coumarylamide for cathepsin H. Procedures were developed that are very convenient, and avoid the hazards associated with the use of naphthylamides.

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Selected References

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  • Singh H, Kalnitsky G. Separation of a new alpha-N-benzoylarginine-beta-naphthylamide hydrolase from cathepsin B1. Purification, characterization, and properties of both enzymes from rabbit lung. J Biol Chem. 1978 Jun 25;253(12):4319–4326. [PubMed]
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