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Biochem J. Aug 15, 1980; 190(2): 461–464.
PMCID: PMC1162113

Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings.

Abstract

An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.

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Selected References

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  • Hamana K, Matsuzaki S. Occurrence of sym-homospermidine in the Japanese newt, Cynops pyrrhogaster pyrrhogaster. FEBS Lett. 1979 Mar 15;99(2):325–328. [PubMed]
  • HERBST EJ, KEISTER DL, WEAVER RH. The separation of aliphatic amines by paper chromatography or paper electrophoresis. Arch Biochem Biophys. 1958 May;75(1):178–185. [PubMed]
  • Inoue H, Mizutani A. A new method for isolation of polyamines from animal tissues. Anal Biochem. 1973 Dec;56(2):408–416. [PubMed]
  • Kupchan SM, Davies AP, Barboutis SJ, Schnoes HK, Burlingame AL. Tumor inhibitors. 43. Solapalmitine and solapalmitenine, two novel alkaloid tumor inhibitors from Solanum tripartitium. J Org Chem. 1969 Dec;34(12):3888–3893. [PubMed]
  • Kuttan R, Radhakrishnan AN. Studies on the biosynthesis of sym-homospermidine in sandal (Santalum album L.). Biochem J. 1972 Mar;127(1):61–67. [PMC free article] [PubMed]
  • Kuttan R, Radhakrishnan AN, Spande T, Witkop B. sym-Homospermidine, a naturally occurring polyamine. Biochemistry. 1971 Feb 2;10(3):361–365. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Oshima T. Thermine: a new polyamine from an extreme thermophile. Biochem Biophys Res Commun. 1975 Apr 21;63(4):1093–1098. [PubMed]
  • Ramakrishna S, Adiga PR. Paper chromatographic resolution of aliphatic guanidino amines, diamines and polyamines. J Chromatogr. 1973 Nov 7;86(1):214–218. [PubMed]
  • Ramakrishna S, Adiga PR. Isolation & characterization of aliphatic amines from Lathyrus sativus seedlings & the changes in their concentrations during development. Indian J Biochem Biophys. 1974 Jun;11(2):128–133. [PubMed]
  • Rolle I, Hobucher H-E, Kneifel H, Paschold B, Riepe W, Soeder CJ. Amines in unicellular green algae. 2. Amines in Scenedesmus acutus. Anal Biochem. 1977 Jan;77(1):103–109. [PubMed]
  • ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. [PubMed]
  • Stillway LW, Walle T. Identification of the unusual polyamines 3,3'-diaminodipropylamine and N,N'-bis(3-aminoproply)-1,3-propanediamine in the white shrimp Penaeus setiferus. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1103–1107. [PubMed]
  • Suresh MR, Adiga PR. Putrescine-sensitive (artifactual) and insensitive (biosynthetic) S-adenosyl-L-methionine decarboxylase activities of Lathyrus sativus seedlings. Eur J Biochem. 1977 Oct 3;79(2):511–518. [PubMed]
  • Tait GH. The formation of homospermidine by an enzyme from Rhodopseudomonas viridis [proceedings]. Biochem Soc Trans. 1979 Feb;7(1):199–201. [PubMed]
  • Yamamoto S, Shinoda S, Makita M. Occurrence of norspermidine in some species of genera Vibrio and Beneckea. Biochem Biophys Res Commun. 1979 Apr 27;87(4):1102–1108. [PubMed]
  • Zappia V, Porta R, Cartenì-Farina M, De Rosa M, Gambacorta A. Polyamine distribution in eukaryotes: occurrence of sym-norm-spermidine and sym-nor-spermine in arthropods. FEBS Lett. 1978 Oct 1;94(1):161–165. [PubMed]

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