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Journal List > J Physiol > v.493(Pt 1); May 15, 1996

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J Physiol. 1996 May 15; 493(Pt 1): 1–8.
PMCID: PMC1158946
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Asynchronous transmitter release: control of exocytosis and endocytosis at the salamander rod synapse.
F Rieke and E A Schwartz
Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637, USA. eas@hearts.bsd.uchicago.edu
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Abstract
1. We have studied exocytosis and endocytosis in the synaptic terminal of salamander rods using a combination of Ca2+ imaging, capacitance measurement and the photolysis of Ca2+ buffers. 2. The average cytoplasmic Ca2+ concentration at the dark resting potential was 2-4 microM. 3. An average cytoplasmic Ca2+ concentration of 2-4 microM maintained a high rate of continuous exocytosis and endocytosis. 4. Changes in the rate of exocytosis were followed in less than 0.7 s by compensatory changes in the rate of endocytosis. 5. Vesicle cycling in the rod synapse is specialized for graded transmission and differs from that previously described for synapses that release synchronized bursts of transmitter.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Augustine GJ, Charlton MP, Smith SJ. Calcium action in synaptic transmitter release. Annu Rev Neurosci. 1987;10:633–693. [PubMed]
  • Barrett EF, Stevens CF. The kinetics of transmitter release at the frog neuromuscular junction. J Physiol. 1972 Dec;227(3):691–708. [PubMed]
  • Capovilla M, Hare WA, Owen WG. Voltage gain of signal transfer from retinal rods to bipolar cells in the tiger salamander. J Physiol. 1987 Oct;391:125–140. [PubMed]
  • Goda Y, Stevens CF. Two components of transmitter release at a central synapse. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12942–12946. [PubMed]
  • Harkins AB, Kurebayashi N, Baylor SM. Resting myoplasmic free calcium in frog skeletal muscle fibers estimated with fluo-3. Biophys J. 1993 Aug;65(2):865–881. [PubMed]
  • Heidelberger R, Heinemann C, Neher E, Matthews G. Calcium dependence of the rate of exocytosis in a synaptic terminal. Nature. 1994 Oct 6;371(6497):513–515. [PubMed]
  • Joshi C, Fernandez JM. Capacitance measurements. An analysis of the phase detector technique used to study exocytosis and endocytosis. Biophys J. 1988 Jun;53(6):885–892. [PubMed]
  • Kado RT. Membrane area and electrical capacitance. Methods Enzymol. 1993;221:273–299. [PubMed]
  • Kaplan JH, Ellis-Davies GC. Photolabile chelators for the rapid photorelease of divalent cations. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6571–6575. [PubMed]
  • Llinás R, Steinberg IZ, Walton K. Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse. Biophys J. 1981 Mar;33(3):323–351. [PubMed]
  • Llinás R, Sugimori M, Silver RB. Microdomains of high calcium concentration in a presynaptic terminal. Science. 1992 May 1;256(5057):677–679. [PubMed]
  • Neher E, Marty A. Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6712–6716. [PubMed]
  • Rieke F, Schwartz EA. A cGMP-gated current can control exocytosis at cone synapses. Neuron. 1994 Oct;13(4):863–873. [PubMed]
  • von Gersdorff H, Matthews G. Inhibition of endocytosis by elevated internal calcium in a synaptic terminal. Nature. 1994 Aug 25;370(6491):652–655. [PubMed]
  • Zucker RS. Effects of photolabile calcium chelators on fluorescent calcium indicators. Cell Calcium. 1992 Jan;13(1):29–40. [PubMed]
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