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Biochem J. 1983 Aug 1; 213(2): 513–518.
PMCID: PMC1152156

Characterization of dolichol and dolichyl phosphate phosphatase from soya beans (Glycine max).


A series of polyprenols, ranging in length from 15 to 22 isoprene units, has been isolated from soya beans (Glycine max) and purified by high-pressure liquid chromatography. N.m.r., i.r. and mass spectra of the compounds indicated that they are alpha-saturated polyprenols of the dolichol type. The amount present in dry seeds was about 9 mg/100 g, whereas dolichyl phosphate (Dol-P) was present only in trace amounts. Dol-P phosphatase activity was detected in the microsomal fraction of 5-day-old germinating soya-bean cotyledons. The Dol-P phosphatase activity was linear with respect to time and protein concentration and exhibited a broad pH optimum (pH 7-9). Triton X-100 was necessary for significant enzyme activity. Enzyme activity was slightly enhanced by EDTA, whereas dithiothreitol was without effect. An apparent Km of 5 microM was determined for Dol-P. Bivalent metal ions were not required for enzyme activity. A number of phosphorylated compounds tested as enzyme substrates (including a number of nucleoside phosphates, glucose 6-phosphate, sodium beta-glycerophosphate and Na4P2O7) did not compete with [1-3H]Dol-P as substrate. A number of phospholipids were also tested for their ability to act as Dol-P phosphatase substrates. At 1 mM concentration, phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and lysophosphatidic acid each inhibited enzymic activity. However, at 0.1 mM concentration, phosphatidylcholine and phosphatidylethanolamine were slightly stimulatory, whereas phosphatidic acid and lysophosphatidic acid were still inhibitory. Phosphatidic acid showed competitive inhibition.

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Selected References

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  • Adrian GS, Keenan RW. Dolichyl phosphate phosphatase from Tetrahymena pyriformis. Biochem J. 1981 Jul 1;197(1):233–238. [PMC free article] [PubMed]
  • Belocopitow E, Boscoboinik D. Dolichyl-phosphate phosphatase and dolichyl-diphosphate phosphatase in rat-liver microsomes. Eur J Biochem. 1982 Jun 15;125(1):167–173. [PubMed]
  • Brett CT, Leloir LF. Dolichyl monophosphate and its sugar derivatives in plants. Biochem J. 1977 Jan 1;161(1):93–101. [PMC free article] [PubMed]
  • Burton WA, Scher MG, Waechter CJ. Enzymatic dephosphorylation of endogenous and exogenous dolichyl monophosphate by calf brain membranes. Arch Biochem Biophys. 1981 May;208(2):409–417. [PubMed]
  • Carroll KK, Vilim A, Woods MC. Polyprenols of beef and human pituitary glands. Lipids. 1973 May;8(5):246–248. [PubMed]
  • Carson DD, Earles BJ, Lennarz WJ. Enhancement of protein glycosylation in tissue slices by dolichylphosphate. J Biol Chem. 1981 Nov 25;256(22):11552–11557. [PubMed]
  • Chadwick CM, Northcote DH. Glucosylation of phosphorylpolyisoprenol and sterol at the plasma membrane of soya-bean (Glycine max) protoplasts. Biochem J. 1980 Feb 15;186(2):411–421. [PMC free article] [PubMed]
  • Chaudhary N, Freeman DJ, Rip JW, Carroll KK. Direct estimation of dolichyl phosphate in rat liver by high pressure liquid chromatography. Lipids. 1982 Aug;17(8):558–560. [PubMed]
  • Cherry JH. Isolation of microsomes, ribosomes, and polysomes from plant tissues. Methods Enzymol. 1974;31:583–589. [PubMed]
  • Delmer DP, Kulow C, Ericson MC. Glycoprotein Synthesis in Plants: II. Structure of the Mannolipid Intermediate. Plant Physiol. 1978 Jan;61(1):25–29. [PMC free article] [PubMed]
  • Hemming FW. Dolichol phosphate, a coenzyme in the glycosylation of animal membrane-bound glycoproteins. Biochem Soc Trans. 1977;5(4):1223–1231. [PubMed]
  • Idoyaga-Vargas V, Belocopitow E, Mentaberry A, Carminatti H. A phosphatase acting on dolichyl phosphate in membranes from neuronal perikarya. FEBS Lett. 1980 Mar 24;112(1):63–66. [PubMed]
  • Lehle L, Fartaczek F, Tanner W, Kauss H. Formation of polyprenol-linked mono- and oligosaccharides in Phaseolus aureus. Arch Biochem Biophys. 1976 Aug;175(2):419–426. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Parodi AJ, Leloir LF. The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell. Biochim Biophys Acta. 1979 Apr 23;559(1):1–37. [PubMed]
  • Lezica RP, Brett CT, Martinez PR, Dankert MA. A glucose acceptor in plants with the properties of an alpha-saturated polyprenyl-monophosphate. Biochem Biophys Res Commun. 1975 Oct 6;66(3):980–987. [PubMed]
  • Rip JW, Rupar CA, Chaudhary N, Carroll KK. Localization of a dolichyl phosphate phosphatase in plasma membranes of rat liver. J Biol Chem. 1981 Feb 25;256(4):1929–1934. [PubMed]
  • Rupar CA, Carroll KK. Occurrence of dolichol in human tissues. Lipids. 1978 Apr;13(4):291–293. [PubMed]
  • Rupar CA, Rip JW, Chaudhary N, Carroll KK. The subcellular localization of enzymes of dolichol metabolism in rat liver. J Biol Chem. 1982 Mar 25;257(6):3090–3094. [PubMed]
  • Sharon N, Lis H. Comparative biochemistry of plant glycoproteins. Biochem Soc Trans. 1979 Aug;7(4):783–799. [PubMed]
  • Staneloni RJ, Tolmasky ME, Petriella C, Ugalde RA, Leloir LF. Presence in a plant of a compound similar to the dolichyl diphosphate oligosaccharide of animal tissue. Biochem J. 1980 Oct 1;191(1):257–260. [PMC free article] [PubMed]
  • Wedgwood JF, Strominger JL. Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate. J Biol Chem. 1980 Feb 10;255(3):1120–1123. [PubMed]

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